Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein, CF Best Seller
Catalog # 193-BC | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Accession #
Source
Structure / Form
Conjugate
Applications
Product Specifications
Source
Human BCMA (Met1-Ala54) Accession # Q6PE46 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
N-terminus | C-terminus |
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
The ED50 for this effect is 0.01-0.04 µg/mL in the presence of 100 ng/mL of rhAPRIL.
Scientific Data Images for Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein, CF
Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein SEC-MALS.
Recombinant Human BCMA/TNFRSF17 Fc Chimera (Catalog # 193-BC) has a molecular weight (MW) of 70.6 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).Bioactivity of Human BCMA / TNFRSF17 Protein
Recombinant human BCMA / TNFRSF17 (193-BC) inhibits APRIL-mediated proliferation of anti-IgM stimulated mouse B cells. The ED50 for this effect is 0.01-0.4 µg/mL in the presence of 100 ng/mL of recombinant human APRIL.Formulation, Preparation and Storage
193-BC
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
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Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: BCMA/TNFRSF17
BCMA, B cell maturation antigen, is a member of the TNF receptor superfamily. It has been designated TNFRSF17. BCMA is a type III membrane protein containing one extracellular cysteine rich domain. Within the TNFRSF, it shares the highest homology with TACI. BCMA and TACI have both been shown to bind to APRIL and BAFF, members of the TNF ligand superfamily. BCMA expression has been found in immune organs and mature B cell lines. Although some expression has been observed at the cell surface, BCMA appears to be localized to the Golgi compartment. The binding of BCMA to APRIL or BAFF has been shown to stimulate IgM production in peripheral blood B cells and increase the survival of cultured B cells. This data suggests that BCMA may play an important role in B cell development, function and regulation. Human BCMA is a 184 amino acid (aa) protein consisting of a 54 aa extracellular domain, a 23 aa transmembrane domain, and a 107 aa intracellular domain. Mouse and human BCMA share 62% amino acid identity.
References
- Madry, C. et al. (1998) Int. Immunol. 10:1693.
- Gras, M. et al. (1995) Int. Immunol. 7:1093.
- Kwon, B. et al. (1999) Curr. Opin. Immunol. 11:340.
- Marsters, S. et al. (2000) Curr. Biol. 10:785.
- Thompson, J. et al. (2000) J. Exp. Med. 192:129.
Long Name
Alternate Names
Gene Symbol
UniProt
Product Documents for Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein, CF
Product Specific Notices for Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein, CF
For research use only
Citations for Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein, CF (4)
Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein, CF include:
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Species: Mouse
Sample Types: Plasma
Applications: ELISA CaptureAR Nanna et al. (2020-06-04), Generation and validation of structurally defined antibody-siRNA conjugates Nucleic Acids Res., 2020-00(0).
PMID: 32347936 -
Species: Human,Rabbit
Sample Types: Serum,Protein
Applications: ELISA CaptureCL Tsai et al. (2020-04-14), Suppressed humoral immunity is associated with dengue nonstructural protein NS1-elicited anti-death receptor antibody fractions in mice Sci Rep, 2020-010(1):6294.
PMID: 32286343 -
Species: Human
Sample Types: Protein
Applications: CAR-T (SPR)DX Bu et al. (2018-05-25), Pre-clinical validation of B cell maturation antigen (BCMA) as a target for T cell immunotherapy of multiple myeloma Oncotarget, 2018-09(40):25764-25780.
PMID: 29899820 -
Species: Human
Sample Types: Protein
Applications: BioassayIsik G et al. (2014-09-23), Enhanced immunogenicity of HIV-1 envelope gp140 proteins fused to APRIL. PLoS ONE, 2014-09(9):e107683.
PMID: 25247707
There are no citations that match your criteria.
Customer Reviews for Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein, CF (2)
Customer Images
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Application: SECVerified Customer | Posted 12/13/2019
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Application: Binding assay/Protein-protein interactionVerified Customer | Posted 11/05/2018
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