Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein, CF Best Seller

Catalog # 193-BC | R&D Systems, Inc. a Bio-Techne Brand
Analyzed by SEC-MALS
Catalog #
Availability
Size / Price
Qty
193-BC-050
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Accession #

Q6PE46

Source

NS0

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human BCMA/TNFRSF17 protein
Human BCMA
(Met1-Ala54)
Accession # Q6PE46		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met1

Predicted Molecular Mass

32 kDa (monomer)

SDS-PAGE

35-45 kDa, reducing conditions

Activity

Measured by its ability to inhibit APRIL-mediated proliferation of anti-IgM stimulated mouse B cells. Moore, P.A. et al. (1999) Science 285:260; Gross, J.A. et al. (2000) Nature 404:995; Schneider, P. et al. (1999) J. Exp. Med. 189:1747.
The ED50 for this effect is 0.01-0.04 µg/mL in the presence of 100 ng/mL of rhAPRIL.

Scientific Data Images for Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein, CF

Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein SEC-MALS.

Recombinant Human BCMA/TNFRSF17 Fc Chimera (Catalog # 193-BC) has a molecular weight (MW) of 70.6 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).
Graph showing dose-dependent bioactivity of Human BCMA protein

Bioactivity of Human BCMA / TNFRSF17 Protein

Recombinant human BCMA / TNFRSF17 (193-BC) inhibits APRIL-mediated proliferation of anti-IgM stimulated mouse B cells. The ED50 for this effect is 0.01-0.4 µg/mL in the presence of 100 ng/mL of recombinant human APRIL.

Formulation, Preparation and Storage

193-BC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BCMA/TNFRSF17

BCMA, B cell maturation antigen, is a member of the TNF receptor superfamily. It has been designated TNFRSF17. BCMA is a type III membrane protein containing one extracellular cysteine rich domain. Within the TNFRSF, it shares the highest homology with TACI. BCMA and TACI have both been shown to bind to APRIL and BAFF, members of the TNF ligand superfamily. BCMA expression has been found in immune organs and mature B cell lines. Although some expression has been observed at the cell surface, BCMA appears to be localized to the Golgi compartment. The binding of BCMA to APRIL or BAFF has been shown to stimulate IgM production in peripheral blood B cells and increase the survival of cultured B cells. This data suggests that BCMA may play an important role in B cell development, function and regulation. Human BCMA is a 184 amino acid (aa) protein consisting of a 54 aa extracellular domain, a 23 aa transmembrane domain, and a 107 aa intracellular domain. Mouse and human BCMA share 62% amino acid identity.

References

  1. Madry, C. et al. (1998) Int. Immunol. 10:1693.
  2. Gras, M. et al. (1995) Int. Immunol. 7:1093.
  3. Kwon, B. et al. (1999) Curr. Opin. Immunol. 11:340.
  4. Marsters, S. et al. (2000) Curr. Biol. 10:785.
  5. Thompson, J. et al. (2000) J. Exp. Med. 192:129.

Long Name

B Cell Maturation Factor

Alternate Names

CD269, TNFRSF13A, TNFRSF17

Entrez Gene IDs

608 (Human); 21935 (Mouse); 287034 (Rat); 102145399 (Cynomolgus Monkey)

Gene Symbol

TNFRSF17

UniProt

Q6PE46

Product Documents for Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein, CF

For research use only

Citations for Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein, CF (4)

Showing  1 - 4 of 4 publications Showing All
Filter By:
Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein, CF include:

There are no citations that match your criteria.

Showing  1 - 4 of 4 publications Showing All

Customer Reviews for Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein, CF (2)

5 out of 5
2 customer ratings
5 stars
100%
4 stars
0%
3 stars
0%
2 stars
0%
1 star
0%

Customer Images

Showing  1 - 2 of 2 reviews Showing All
Filter By:
  • Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein, CF
    Name: Anonymous
    Application: SEC
    Verified Customer | Posted 12/13/2019
    Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein, CF 193-BC
  • Recombinant Human BCMA/TNFRSF17 Fc Chimera Protein, CF
    Name: Jenna Nguyen
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 11/05/2018

There are no reviews that match your criteria.

Showing  1 - 2 of 2 reviews Showing All

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

÷
=
Setting the standard in quality research reagents