Recombinant Human B7-1/CD80 Fc Chimera His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 140-B1

Discontinued Product
140-B1 has been discontinued. An alternative/replacement product is available: 10133-B1, 9050-B1. View all B7-1/CD80 products.

Key Product Details

Source

NS0

Accession #

NP_005182

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all B7-1/CD80 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human B7-1/CD80 protein
Human B7-1
(Val35-Asn242)
Accession # NP_005182		 IEGRMD Human IgG1
(Pro100-Lys330)		 6-His tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val35

Predicted Molecular Mass

51.3 kDa (monomer)

SDS-PAGE

70-90 kDa, reducing conditions

Activity

Measured by its ability to induce IL-2 secretion by Jurkat human acute T cell leukemia cells. Freeman, G.J. et al. (1993) Science 262:909.
The ED50 for this effect is 0.025-0.15 µg/mL in the presence of PHA.

Reviewed Applications

Read 2 reviews rated 5 using 140-B1 in the following applications:

Formulation, Preparation and Storage

140-B1
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: B7-1/CD80

B7-1/CD80 and B7-2/CD86, together with their receptors CD28 and CTLA-4, constitute one of the dominant co-stimulatory pathways that regulate T- and B-cell responses (1). Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28 and is involved in the down-regulation of the immune response (2-6). Mature human B7-1 consists of a 208 aa extracellular domain (ECD) with two immunoglobulin-like domains, a 21 aa transmembrane domain, and a 25 aa cytoplasmic domain (7). Within the ECD, human B7-1 shares 50% aa sequence identity with mouse and rat
B7-1. Alternative splicing generates a 30 kDa soluble isoform that lacks the transmembrane segment and retains the ability to bind CD28 and CTLA-4 and an isoform that lacks the second Ig-like domain and the transmembrane segment (8). Both human and mouse B7-1 and B7-2 can bind to either human or mouse CD28 and
CTLA-4 (1). B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells (2).

References

  1. Ville, S. et al. (2015) Front. Immunol. 6:411.
  2. Azuma, M. et al. (1993) Nature 366:76.
  3. Freeman, G.J. et al. (1993) Science 262:909.
  4. Chen, C. et al. (1994) J. Immunol. 152:4929.
  5. Freeman, G.J. et al. (1993) J. Exp. Med. 178:2185.
  6. Lanier, L. et al. (1995) J. Immunol. 154:97.
  7. Freeman, G.J. et al. (1989) J. Immunol. 143:2714.
  8. Kakoulidou, M. et al. (2007) Scand. J. Immunol. 66:529.

Alternate Names

B71, CD80

Entrez Gene IDs

941 (Human); 12519 (Mouse); 25408 (Rat); 102140078 (Cynomolgus Monkey)

Gene Symbol

CD80

UniProt

NP_005182

Additional B7-1/CD80 Products

Product Documents for Recombinant Human B7-1/CD80 Fc Chimera His-tag Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human B7-1/CD80 Fc Chimera His-tag Protein, CF

For research use only

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