Phalloidin-FITC
Tocris Bioscience, part of Bio-Techne | Catalog # 5782
Green-fluorescent cytoskeleton stain. Binds and labels F-actin. Used for: F-actin staining. Application: fluorescent microscopy, immunofluorescence (IHC, ICC)
Key Product Details
Description
Green-fluorescent cytoskeleton stain. Binds and labels F-actin. Used for: F-actin staining. Application: fluorescent microscopy, immunofluorescence (IHC, ICC)
Product Description
Key information: Phalloidin-FITC is a green-fluorescent cytoskeleton stain. It binds and labels specifically F-actin.Used for: Phalloidin-FITC can be used to visualize and quantify F-actin in tissue sections, cell cultures or cell-free experiments with optimal results in formaldehyde-fixed and permeabilized cells.
Application: fluorescent microscopy, immunofluorescence (IHC, ICC).
Properties and Photophysical Data: Phalloidin-FITC is composed of the F-actin probe, Phalloidin (Cat. No. 4535), conjugated to Fluorescein isothiocyanate (FITC; Cat. No. 5440) dye. Phalloidin binds F-actin with high selectivity allowing Phalloidin-FITC to stain actin with high-contrast discrimination. Excitation and emission maxima (λ) are 496 nm and 516 nm, respectively.
We suggest making up a stock solution of this product by dissolving the vial contents in 1.5 mL methanol or DMSO.
For more information on how to use Phalloidin-FITC, please see our protocol.
Scientific Data Images for Phalloidin-FITC
Applications in HeLa Cells for Phalloidin-FITC.
Application of Phalloidin-FITC: HeLa cells were labeled with 150 nM Phalloidin-FITC for 20 minutes. Counterstained with DAPI (Catalog # 5748).Optical Data
| λabs | 496 nm |
| λem | 516 nm |
| Application | Fluorescent Microscopy, Immunofluorescence (IHC, ICC). |
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Product Specifications
Molecular Weight
1177.26
Formula
C56H60N10O15S2
Storage
Store at -20°C
Purity
≥90% (HPLC)
Chemical Name
7-[(4R)-N5-[[(3',6'-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl)amino]thioxomethyl]-4-hydroxy-4-methyl-L-ornithine]phalloidin
CAS Number
915026-99-2
PubChem ID
131676694
InChI Key
CJEWZWZNRFVJSM-PNHWZQMUSA-N
SMILES
C[C@H](O)[C@H]1NC(=O)C(C)NC(=O)[C@H](C[C@@](C)(O)CNC(=S)NC2=CC(C(O)=O)=C(C=C2)C2=C3C=CC(=O)C=C3OC3=C2C=CC(O)=C3)NC(=O)[C@@H]2CC3=C(NC4=C3C=CC=C4)SC[C@@H](NC1=O)C(=O)N1C[C@H](O)C[C@H]1C(=O)N[C@@H](C)C(=O)N2
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
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Product Specific Notices for Phalloidin-FITC
For research use only
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