Phalloidin-FITC

Name: Phalloidin-FITC
Brand: Tocris Bioscience
SKU: 5782

Tocris Bioscience, part of Bio-Techne | Catalog # 5782

COA / SDS

Green-fluorescent cytoskeleton stain. Binds and labels F-actin. Used for: F-actin staining. Application: fluorescent microscopy, immunofluorescence (IHC, ICC)

Key Product Details

Description:	Green-fluorescent cytoskeleton stain. Binds and labels F-actin. Used for: F-actin staining. Application: fluorescent microscopy, immunofluorescence (IHC, ICC)
Chemical Name:	7-[(4R)-N⁵-[[(3',6'-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl)amino]thioxomethyl]-4-hydroxy-4-methyl-L-ornithine]phalloidin
Purity:	≥90% (HPLC)
Molecular Weight:	1177.26

View Full Technical Details for Phalloidin-FITC

Technical Data
Citations

Biological Activity for Phalloidin-FITC

Key information: Phalloidin-FITC is a green-fluorescent cytoskeleton stain. It binds and labels specifically F-actin.

Used for: Phalloidin-FITC can be used to visualize and quantify F-actin in tissue sections, cell cultures or cell-free experiments with optimal results in formaldehyde-fixed and permeabilized cells.

Application: fluorescent microscopy, immunofluorescence (IHC, ICC).

Properties and Photophysical Data: Phalloidin-FITC is composed of the F-actin probe, Phalloidin (Cat. No. 4535), conjugated to Fluorescein isothiocyanate (FITC; Cat. No. 5440) dye. Phalloidin binds F-actin with high selectivity allowing Phalloidin-FITC to stain actin with high-contrast discrimination. Excitation and emission maxima (λ) are 496 nm and 516 nm, respectively.

We suggest making up a stock solution of this product by dissolving the vial contents in 1.5 mL methanol or DMSO.

For more information on how to use Phalloidin-FITC, please see our protocol.

Optical Data for Phalloidin-FITC

λ_abs	496 nm
λ_em	516 nm
Application	Fluorescent Microscopy, Immunofluorescence (IHC, ICC).

Scientific Data Examples for Phalloidin-FITC

	Applications in HeLa Cells for Phalloidin-FITC. Application of Phalloidin-FITC: HeLa cells were labeled with 150 nM Phalloidin-FITC for 20 minutes. Counterstained with DAPI (Catalog # 5748).

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Technical Data for Phalloidin-FITC

M. Wt	1177.26
Formula	C₅₆H₆₀N₁₀O₁₅S₂
Storage	Store at -20°C
Purity	≥90% (HPLC)
CAS Number	915026-99-2
PubChem ID	131676694
InChi Key	CJEWZWZNRFVJSM-PNHWZQMUSA-N
Smiles	C[C@H](O)[C@H]1NC(=O)C(C)NC(=O)[C@H](C[C@@](C)(O)CNC(=S)NC2=CC(C(O)=O)=C(C=C2)C2=C3C=CC(=O)C=C3OC3=C2C=CC(O)=C3)NC(=O)[C@@H]2CC3=C(NC4=C3C=CC=C4)SC[C@@H](NC1=O)C(=O)N1C[C@H](O)C[C@H]1C(=O)N[C@@H](C)C(=O)N2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

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Product Documents for Phalloidin-FITC

COA

Certificate of Analysis

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