BDY FL-X, SE
Tocris Bioscience, part of Bio-Techne | Catalog # 5620
Green BDY (BODIPY® or boron-dipyrromethene) fluorescent dye for the labeling of amines; supplied as NHS ester
Discontinued Product
5620 has been discontinued.
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Key Product Details
Description
Green BDY (BODIPY® or boron-dipyrromethene) fluorescent dye for the labeling of amines; supplied as NHS ester
Product Description
BDY FL-X, SE is a fluorescent green BDY (BODIPY® or boron-dipyrromethene) dye for the labeling of amines. Exhibits similar excitation and emission spectra to FITC and TFAX 488 dyes. Displays high fluorescence quantum yield and high extinction coefficient and is relatively insensitive to pH change. This dye contains a C3 alkyl spacer between the fluorophore and the NHS ester group, reducing the potential for interactions between fluorophore and conjugated biomolecule. The hydrophobic nature of BDY FL makes it ideal for labeling lipids and cell membranes. BDY FL also exhibits narrow emission bandwidths and has a relatively long excited-state lifetime, making it ideal for fluorescence polarization assays and two-photon excitation (TPE) microscopy. Excitation maximum ~504 nM; emission maximum ~510 nM.
BODIPY® is a registered trademark of Molecular Probes.
Optical Data
| Emission Color | Green |
| λabs | 504 nm |
| λem | 510 nm |
| Extinction Coefficient (ε) | 85000 M-1cm-1 |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | No |
| Application | Confocal fluorescence p, fluorescence polarization assays and two-photon excitation (TPE) microscopy |
| Alternative For | FAM; Alexa Fluor 488; DyLight 488; Cy2 |
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Product Specifications
Molecular Weight
502.32
Formula
C24H29BF2N4O5
Storage
Store at -20°C
Purity
≥90% (HPLC)
Chemical Name
(T-4)-[5-[(3,5-Dimethyl-2H-pyrrol-2-ylidene-&N)methyl]-[N-[6-[(2,5-Dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-1H-pyrrole-2-propanamidato-κN1]difluoroboron
CAS Number
217190-09-5
PubChem ID
13007361
InChI Key
AWACOFBGFAKYPF-UHFFFAOYSA-N
SMILES
CC(C=C1C)=C2N1[B-](F)(F)[N+]3=C(CCC(NCCCCCC(ON4C(CCC4=O)=O)=O)=O)C=CC3=C2
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 50.23 | 100 |
Preparing Stock Solutions
for BDY FL-X, SE
The following data is based on the product molecular weight 502.32
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.99 mL | 9.95 mL | 19.91 mL |
| 5 mM | 0.40 mL | 1.99 mL | 3.98 mL |
| 10 mM | 0.20 mL | 1.00 mL | 1.99 mL |
| 50 mM | 0.04 mL | 0.20 mL | 0.40 mL |
Calculators
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Background References
References are publications that support the biological activity of the product.
- Baker Influence of fluorophore and linker composition on the pharmacology of fluorescent adenosine A1 receptor ligands. Br.J.Pharmacol. 2010 PMID: 20105183
Product Specific Notices for BDY FL-X, SE
For research use only
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