Human sTransferrin Receptor/sTfR Quantikine ELISA, RUO
R&D Systems, part of Bio-Techne | Catalog # DTFRU0
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Serum (25 µL), EDTA Plasma (50 µL), Heparin Plasma (25 µL)
Sensitivity
0.064 nmol/L
Assay Range
1.25-40 nmol/L (Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human sTransferrin Receptor/sTfR Quantikine ELISA, RUO
The Quantikine Human sTfR Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human sTfR in serum and plasma. Results obtained using natural human sTfR showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human sTfR.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Specificity
This assay recognizes natural human sTfR.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (nmol/L) | 4.82 | 18.4 | 28.8 | 5.02 | 19.8 | 29.5 |
| Standard Deviation | 0.115 | 0.375 | 0.791 | 0.202 | 1.31 | 2.56 |
| CV% | 2.4 | 2.0 | 2.7 | 4.0 | 6.6 | 8.7 |
Recovery for Human sTransferrin Receptor/sTfR Quantikine ELISA, RUO
The recovery of human sTfR spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 107 | 98-114 |
| Heparin Plasma (n=4) | 107 | 96-119 |
| Serum (n=4) | 109 | 94-116 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of human sTfR in various matrices were diluted with the calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human sTransferrin Receptor/sTfR Quantikine ELISA, RUO
Human sTfR Quantikine ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: TfR (Transferrin R)
Transferrin Receptor (TfR, TfR-1, CD71) is a transmembrane glycoprotein expressed on erythroid progenitors, muscle cells and proliferating cells as a disulfide-linked homodimer. As the major mediator of cellular iron uptake, it binds and internalizes diferric transferrin, allowing iron release at the low pH of the endosome. Proteolytic shedding of TfR into the circulation is enhanced by iron deficiency. The hereditary hemochromatosis protein HFE competes with diferric transferrin for binding to TfR and targets TfR for degradation rather than recycling. Cancer cells can express up to 5-fold more TfR than quiescent cells in the surrounding tissue. TfR carries out ferritin-independent functions in T cell development, immunological synapse formation and Galectin-3-mediated cell death, and it serves as a cell entry receptor for New World hemorrhagic fever arenaviruses.
Long Name
Transferrin Receptor
Alternate Names
CD71, TfR (TransferrinR), TFR1, TFRC, TRFR
Gene Symbol
TFRC
Additional TfR (Transferrin R) Products
Product Documents for Human sTransferrin Receptor/sTfR Quantikine ELISA, RUO
Product Specific Notices for Human sTransferrin Receptor/sTfR Quantikine ELISA, RUO
For research use only
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