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Human IFN-gamma ELISA Kit - Quantikine

Bio-Techne includes R&D Systems | Catalog # DIF50

This product has been discontinuation and replaced by catalog # DIF50C.
Discontinued Product
DIF50 has been discontinued and is replaced by DIF50C.

Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL)


8 pg/mL

Assay Range

15.6-1000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)

Product Summary for Human IFN-gamma Quantikine ELISA Kit

The Quantikine Human IFN-gamma Immunoassay is a 4.5 hour solid phase ELISA designed to measure IFN-gamma levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IFN-gamma and antibodies raised against the recombinant factor. Results obtained for naturally occurring human IFN-gamma samples showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IFN-gamma.

Product Specifications


Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)






Natural and recombinant human IFN-gamma



No significant interference observed with available related molecules.


Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, EDTA Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 79.2 214 466 78.7 206 457
Standard Deviation 3.7 5.5 13.0 6.1 13.1 17.0
CV% 4.7 2.6 2.8 7.8 6.4 3.7

Recovery for Human IFN-gamma Quantikine ELISA Kit

The recovery of IFN-gamma spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 102 93-109
EDTA Plasma (n=5) 98 88-111
Serum (n=5) 102 91-118


To assess the linearity of the assay, five samples were spiked with high concentrations of IFN-gamma in various matrices and diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human IFN-gamma ELISA Linearity
Human IFN-gamma  Linearity Curve Quantikine ELISA

Scientific Data Images for Human IFN-gamma Quantikine ELISA Kit

Human IFN-gamma ELISA Standard Curve

Human IFN-gamma ELISA Standard Curve

Human IFN-gamma  Standard Curve ELISA

Human IFN-gamma Standard Curve ELISA

Preparation and Storage


The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IFN-gamma

IFN-gamma (Interferon-gamma) is the prototype proinflammatory cytokine and is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. IFN-gamma dimers signal through a receptor complex of two IFN-gamma R1 and two IFN-gamma R2 subunits.

Long Name

Interferon gamma

Alternate Names


Entrez Gene IDs

3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)

Gene Symbol


Additional IFN-gamma Products

Product Documents for Human IFN-gamma Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human IFN-gamma Quantikine ELISA Kit

For research use only


⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to