Assay Diluent RD1-63 contains visible precipitate. Is this acceptable to use?

Name: Human IFN-gamma Quantikine ELISA Kit
Brand: R&D Systems
SKU: DIF50C
Rating: 5 (10 reviews)

A small amount of precipitate is expected and acceptable in Assay Diluent RD1-63. Variability has been observed between vials and diluent lots. It may not fully resolubilize but this will not impact assay performance. It is not recommended to filter the precipitate.

Human IFN-gamma Quantikine ELISA Kit Best Seller

Catalog # DIF50C | R&D Systems, Inc. a Bio-Techne Brand

(10)

Citations (205)

Product Datasheet / COA / SDS

Setting the standard in quality research reagents

Key Product Details

Assay Length:	4.5 hours
Sample Type & Volume Required Per Well:	Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
Sensitivity:	5.69 pg/mL
Assay Range:	15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Human IFN-gamma Quantikine ELISA Kit Summary:	The Quantikine Human IFN-gamma Immunoassay is a 4.5 hour solid phase ELISA designed to measure human IFN-gamma levels in cell culture supernates, serum, and plasma. It contains HEK293-expressed recombinant human IFN-gamma and antibodies raised against the recombinant factor. Results obtained for naturally occurring human IFN-gamma samples showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IFN-gamma.

View Full Technical Details for Human IFN-gamma Quantikine ELISA Kit

Product Specifications for Human IFN-gamma Quantikine ELISA Kit

Measurement	Quantitative ELISA
Detection Method	Colorimetric - 450nm (TMB)
Conjugate	HRP
Reactivity	Human
Specificity	Natural and recombinant human IFN-gamma.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	153	366	720	154	366	723
Standard Deviation	3.47	9.16	19.4	9.9	20.6	54
CV%	2.3	2.5	2.7	6.4	5.6	7.5

Recovery

The recovery of human IFN-gamma spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	91	84-106
EDTA Plasma (n=4)	95	85-105
Heparin Plasma (n=4)	96	87-101
Serum (n=4)	95	90-109

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human IFN-gamma in various matrices were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Examples for Human IFN-gamma Quantikine ELISA Kit

	Human IFN-gamma ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IFN-gamma

Interferon-gamma (IFN-gamma ) is an important immunomodulatory cytokine, affecting both the innate and adaptive immune systems. It was discovered in 1965 as a soluble anti-viral factor and has since been shown to promote host defense against a wide variety of pathogens (1, 2). Additionally, it has been shown to promote autophagy and apoptosis, and to have anti-proliferative, anti-angiogenic, and anti-tumorigenic properties (1, 3, 4). IFN-gamma is primarily secreted by natural killer (NK) cells (5-7), activated CD8+ T cells (8), Th1 CD4+ T cells (9), NKT cells (10, 11), and macrophages (12-16), but it has also been shown to be produced by a number of other cell types including dendritic cells (17), gamma δ T cells (18), group 1 ILCs (19), keratinocytes (20), neutrophils (21), mast cells (22), and neurons (23).

The biologically active form of IFN-gamma is a non-covalently linked homodimer (24), which binds with high affinity to IFN-gamma R1/CD119 and subsequently recruits IFN-gamma R2 to form the functional heterotetrameric receptor complex. Formation of this complex leads to phosphorylation and activation of the Janus kinases, Jak1 and Jak2, which in turn phosphorylate and activate STAT1. STAT1 homodimerizes and translocates to the nucleus where it binds to IFN-gamma -activated sequence (GAS) elements in the promoters of target genes to regulate their transcription. Many of the IFN-gamma /STAT1 target genes are transcription factors that then drive the expression of secondary response genes. Additionally, IFN-gamma signaling has been shown to activate MAPK, PI 3-K/Akt, and the NF-kappa B signaling pathways, leading to the expression of multiple other genes. IFN-gamma signaling plays a key role in host defense by promoting macrophage activation, upregulating the expression of antigen processing and presentation molecules, driving the development and activation of Th1 cells, enhancing natural killer cell activity, regulating B cell functions, and inducing the production of chemokines that promote effector cell trafficking to sites of inflammation.

Due to its immunoregulatory activities, IFN-gamma has been used as a therapeutic agent for treating a range of bacterial, fungal, helminth, protozoan, and viral infections, immunodeficiency syndromes, multi-drug resistant tuberculosis (MDR-TB), and sepsis (1, 25-34). Additionally, it has been used as an anti-tumor agent to improve patient survival in a number of different types of cancer due to its pro-apoptotic and anti-angiogenic effects (3, 35). In contrast, IFN-gamma has been suggested to be involved in the progression of cardiac diseases as elevated levels of this cytokine have been detected in the serum of patients with chronic heart failure, as well as in atherosclerotic lesions and in myocardial tissues of patients with Chagas' cardiomyopathy (1, 36, 37). Similarly, high levels of IFN-gamma have been found in the serum and/or cerebrospinal fluid of patients with neurodegenerative diseases such as Amyotrophic lateral sclerosis and Parkinson's disease (38, 39), suggesting that IFN-gamma may also be involved in neurodegenerative disease progression and serve as a clinical biomarker. Additionally, there is recent evidence suggesting that IFN-gamma may also have context-dependent proliferative and pro-tumorigenic effects (3).

Item	Value
Long Name	Interferon gamma
Entrez Gene IDs	3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
Alternate Names	IFG, IFI, IFN-gamma, IFNG, IFNgamma, Immune interferon, interferon gamma, interferon, gamma

Citations for Human IFN-gamma Quantikine ELISA Kit (205)

Showing 1 - 10 of 205 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Human IFN-gamma Quantikine ELISA Kit include:

Species: Human
Sample Types: Serum

Abramiuk et al. (2023-04-24), Possible Correlation between Urocortin 1 (Ucn1) and Immune Parameters in Patients with Endometriosis International journal of molecular sciences
PMID: 37175494
Species: Human
Sample Types: Cell Culture Supernates

G Silva et al. (2023-03-02), Novel scFv against Notch Ligand JAG1 Suitable for Development of Cell Therapies toward JAG1-Positive Tumors Biomolecules, 2023-013(3).
PMID: 36979394
Species: Human
Sample Types: Plasma

AG Saleh et al. (2023-02-27), Key cytokines and hematological parameters in patients with uncomplicated falciparum malaria in Hodeidah, Yemen Cytokine, 2023-0164(0):156156.
PMID: 36857851
Species: Human
Sample Types: Cell Culture Supernates

F Parween et al. (2023-02-13), Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells bioRxiv : the preprint server for biology, 2023-00(0).
PMID: 36789428
Species: Human
Sample Types: Cell Culture Supernates

SY Cho et al. (2023-01-12), WT1 Pulsed Human CD141+ Dendritic Cell Vaccine Has High Potential in Solid Tumor-Targeted Immunotherapy International Journal of Molecular Sciences, 2023-024(2).
PMID: 36675017
Species: Human
Sample Types: Plasma

MC Giménez et al. (2022-12-29), Effects of Near-Infrared Light on Well-Being and Health in Human Subjects with Mild Sleep-Related Complaints: A Double-Blind, Randomized, Placebo-Controlled Study Biology, 2022-112(1).
PMID: 36671752
Species: Human
Sample Types: Cell Culture Supernates.

A Barilli et al. (2022-12-01), Growth Arrest of Alveolar Cells in Response to Cytokines from Spike S1-Activated Macrophages: Role of IFN-gamma Biomedicines, 2022-110(12).
PMID: 36551841
Species: Human
Sample Types: Cell Culture Supernates

P Yuti et al. (2022-11-23), Anti-CD19 chimeric antigen receptor T cells secreting anti-PD-L1 single-chain variable fragment attenuate PD-L1 mediated T cell inhibition International immunopharmacology, 2022-1113(0):109442.
PMID: 36435066
Species: Human
Sample Types: Cell Culture Supernates

R Yoshida et al. (2022-11-02), MET-induced CD73 restrains STING-mediated immunogenicity of EGFR-mutant lung cancer Cancer Research, 2022-10(0).
PMID: 36066413
Species: Human
Sample Types: Serum

CV Gonçalves et al. (2022-10-28), Inflammaging and body composition: New insights in diabetic and hypertensive elderly men Experimental gerontology, 2022-1170(0):112005.
PMID: 36341786
Species: Human
Sample Types: Serum

J Milosevic et al. (2022-09-13), Potential Protective Role of Galectin-3 in Patients with Gonarthrosis and Diabetes Mellitus: A Cross-Sectional Study International Journal of Environmental Research and Public Health, 2022-019(18).
PMID: 36141752
Species: Human
Sample Types: Cell Culture Supernates

Y Bian et al. (2022-07-30), The Generation of Dual-Targeting Fusion Protein PD-L1/CD47 for the Inhibition of Triple-Negative Breast Cancer Biomedicines, 2022-010(8).
PMID: 36009390
Species: Human
Sample Types: Serum

MPL Galantini et al. (2022-07-30), The sweet fuel of inflammation: New perspectives on the complex web that interconnects diabetes Experimental gerontology, 2022-0167(0):111905.
PMID: 35918042
Species: Human
Sample Types: Cell Culture Supernates

W Singhatana et al. (2022-07-27), IFNgamma-primed periodontal ligament cells regulate T-cell responses via IFNgamma-inducible mediators and ICAM-1-mediated direct cell contact Royal Society open science, 2022-09(7):220056.
PMID: 35911203
Species: Mouse
Sample Types: Cell Culture Supernates

S Tomar et al. (2022-07-05), Development of Highly Effective Anti-Mesothelin hYP218 Chimeric Antigen Receptor T Cells With Increased Tumor Infiltration and Persistence for Treating Solid Tumors Molecular Cancer Therapeutics, 2022-021(7):1195-1206.
PMID: 35499461
Species: Human
Sample Types: Cell Culture Supernates

J Saetang et al. (2022-07-05), Surface cysteine to serine substitutions in IL-18 reduce aggregation and enhance activity PeerJ, 2022-010(0):e13626.
PMID: 35811828
Species: Human
Sample Types: Cell Culture Supernates

N Mohan et al. (2022-06-29), Comparative Characterization of Different Molecular Formats of Bispecific Antibodies Targeting EGFR and PD-L1 Oncogene, 2022-014(7).
PMID: 35890277
Species: Human
Sample Types: Cell Culture Supernates

TA Karginov et al. (2022-06-20), Optimal CD8+ T cell effector function requires costimulation-induced RNA-binding proteins that reprogram the transcript isoform landscape Nature Communications, 2022-013(1):3540.
PMID: 35725727
Species: Human
Sample Types: Cell Culture Supernates

N Jirapongwa et al. (2022-05-26), Mesothelin?specific T cell cytotoxicity against triple negative breast cancer is enhanced by 40s ribosomal protein subunit 3?treated self?differentiated dendritic cells Oncology reports, 2022-048(1).
PMID: 35616135
Species: Human
Sample Types: Cell Culture Supernates

G Rodionov et al. (2022-05-10), Short treatment of peripheral blood cells product with Fas ligand using closed automated cell processing system significantly reduces immune cell reactivity of the graft in vitro and in vivo Bone marrow transplantation, 2022-00(0).
PMID: 35538142
Species: Human
Sample Types: Serum

S Luo et al. (2022-05-10), Epigenetic Regulation of IFI44L Expression in Monocytes Affects the Functions of Monocyte-Derived Dendritic Cells in Systemic Lupus Erythematosus Journal of Immunology Research, 2022-02022(0):4053038.
PMID: 35592687
Species: Human
Sample Types: Tissue Homogenates

C Zhong et al. (2022-02-18), HOXA-AS2 contributes to regulatory T cell proliferation and immune tolerance in glioma through the miR-302a/KDM2A/JAG1 axis Cell Death Disease, 2022-02-18;13(2):160.
PMID: 35181676
Species: Human
Sample Types: Serum

C Udjus et al. (2022-02-01), Extreme altitude induces divergent mass reduction of right and left ventricle in mountain climbers Physiological Reports, 2022-010(3):e15184.
PMID: 35146955
Species: Human
Sample Types: Serum

B Orts et al. (2022-01-03), Clinical and Immunological Factors Associated with Recommended Trough Levels of Adalimumab and Infliximab in Patients with Crohn's Disease Frontiers in Pharmacology, 2022-012(0):795272.
PMID: 35046819
Species: Human
Sample Types: Cell Culture Supernates

VR Ribeiro et al. (2021-11-20), Vitamin D modulates the transcription factors of T cell subsets to anti-inflammatory and regulatory profiles in preeclampsia International immunopharmacology, 2021-10(0):108366.
PMID: 34810124
Species: Human
Sample Types: Serum

WS Khalaf et al. (2021-11-16), Phenotypic characterization of NKT-like cells and evaluation of specifically related cytokines for the prediction of unexplained recurrent miscarriage Heliyon, 2021-17(11):e08409.
PMID: 34849421
Species: Human
Sample Types: Plasma

K Kurianowic et al. (2021-11-09), Impaired Innate Immunity in Pediatric Patients Type 1 Diabetes-Focus on Toll-like Receptors Expression International Journal of Molecular Sciences, 2021-122(22).
PMID: 34830017
Species: Human
Sample Types: Serum

MT Guagnano et al. (2021-10-09), Improvement of Inflammation and Pain after Three Months' Exclusion Diet in Rheumatoid Arthritis Patients Nutrients, 2021-113(10).
PMID: 34684536
Species: Human
Sample Types: Plasma

IS Ribeiro et al. (2021-09-20), Regular physical activity reduces the effects of inflammaging in diabetic and hypertensive men Experimental gerontology, 2021-0155(0):111558.
PMID: 34547405
Species: Human
Sample Types: Cell Culture Supernates

MS Hwang et al. (2021-09-06), Structural engineering of chimeric antigen receptors targeting HLA-restricted neoantigens Nature Communications, 2021-012(1):5271.
PMID: 34489470
Species: Human
Sample Types: Serum

KS Lee et al. (2021-09-04), Interferon-gamma-Inducible Chemokines as Prognostic Markers for Lung Cancer International Journal of Environmental Research and Public Health, 2021-018(17).
PMID: 34501934
Species: Human
Sample Types: Cell Culture Supernates

S Nakajima et al. (2021-08-19), The effects of T-DXd on the expression of HLA class I and chemokines CXCL9/10/11 in HER2-overexpressing gastric cancer cells Scientific Reports, 2021-011(1):16891.
PMID: 34413454
Species: Human
Sample Types: Cell Culture Supernates

J Petersson et al. (2021-08-06), Bone Marrow Neutrophils of Multiple Myeloma Patients Exhibit Myeloid-Derived Suppressor Cell Activity Journal of Immunology Research, 2021-02021(0):6344344.
PMID: 34414242
Species: Cynomolgus Monkey, Human
Sample Types: Cell Culture Supernates

C Geuijen et al. (2021-07-21), A human CD137ï¿½PD-L1 bispecific antibody promotes anti-tumor immunity via context-dependent T cell costimulation and checkpoint blockade Nature Communications, 2021-012(1):4445.
PMID: 34290245
Species: Human
Sample Types: Cell Culture Supernates

Y Chen et al. (2021-07-21), Engineered extracellular vesicles for concurrent Anti-PDL1 immunotherapy and chemotherapy Bioactive materials, 2021-09(0):251-265.
PMID: 34820569
Species: Human
Sample Types: Plasma

RL Relf et al. (2021-07-01), Thermoregulation is not impaired in breast cancer survivors during moderate-intensity exercise performed in warm and hot environments Physiological Reports, 2021-09(14):e14968.
PMID: 34291605
Species: Human
Sample Types: Cell Culture Supernates

CF Huang et al. (2021-06-15), Dose-Related Aberrant Inhibition of Intracellular Perforin Expression by S1 Subunit of Spike Glycoprotein That Contains Receptor-Binding Domain from SARS-CoV-2 Microorganisms, 2021-09(6).
PMID: 34203929
Species: Human
Sample Types: Plasma

M Tomori et al. (2021-06-15), Effects of Ingesting Fucoidan Derived from Cladosiphon okamuranus Tokida on Human NK Cells: A Randomized, Double-Blind, Parallel-Group, Placebo-Controlled Pilot Study Marine Drugs, 2021-019(6).
PMID: 34203925
Species: Human
Sample Types: Plasma

DPS Lopes et al. (2021-06-11), Regular physical activity reduces the proinflammatory response in older women with diabetes and hypertension in the postmenopausal phase Experimental gerontology, 2021-0152(0):111449.
PMID: 34126225
Species: Human
Sample Types: Cell Culture Supernates

JA Jarrell et al. (2021-06-03), Numerical optimization of microfluidic vortex shedding for genome editing T cells with Cas9 Scientific Reports, 2021-011(1):11818.
PMID: 34083685
Species: Human
Sample Types: Plasma

Y Jiang et al. (2021-05-17), Socioeconomic status, financial stress, and glucocorticoid resistance among youth with asthma: Testing the moderation effects of maternal involvement and warmth Brain, Behavior, and Immunity, 2021-00(0).
PMID: 34015429
Species: Human
Sample Types: Cell Culture Supernates

AM Aydin et al. (2021-02-23), Expansion of tumor-infiltrating lymphocytes (TIL) from penile cancer patients International immunopharmacology, 2021-094(0):107481.
PMID: 33636562
Species: Human
Sample Types: Plasma

BK Jeong et al. (2021-02-11), Cytokine Profiles of Non-Small Cell Lung Cancer Patients Treated with Concurrent Chemoradiotherapy with Regards to Radiation Pneumonitis Severity Journal of Clinical Medicine, 2021-010(4).
PMID: 33670117
Species: Human
Sample Types: Cell Culture Supernates

Y Cheng et al. (2021-01-15), The anti-tumor effects of cetuximab in combination with VTX-2337 are T cell dependent Scientific Reports, 2021-011(1):1535.
PMID: 33452311
Species: Human
Sample Types: Cell Culture Supernates

Y Wang et al. (2021-01-14), SHP2 blockade enhances anti-tumor immunity via tumor cell intrinsic and extrinsic mechanisms Scientific Reports, 2021-011(1):1399.
PMID: 33446805
Species: Human
Sample Types: Cell Culture Supernates

F Gaiani et al. (2020-11-20), The Diverse Potential of Gluten from Different Durum Wheat Varieties in Triggering Celiac Disease: A Multilevel In Vitro, Ex Vivo and In Vivo Approach Nutrients, 2020-112(11).
PMID: 33233787
Species: Human
Sample Types: Cell Culture Supernates

A Sa?kowska et al. (2020-11-12), SARS-CoV-2 Proteins Induce IFNG in Th1 Lymphocytes Generated from CD4+ Cells from Healthy, Unexposed Polish Donors Vaccines (Basel), 2020-18(4).
PMID: 33198287
Species: Human
Sample Types: Plasma

A Sacchi et al. (2020-10-27), Early expansion of myeloid-derived suppressor cells inhibits SARS-CoV-2 specific T-cell response and may predict fatal COVID-19 outcome Cell Death Dis, 2020-111(10):921.
PMID: 33110074
Species: Human
Sample Types: Cell Culture Supernates

K Yamashita et al. (2020-10-26), Trastuzumab upregulates programmed death ligand-1 expression through interaction with NK cells in gastric cancer Br J Cancer, 2020-10(0).
PMID: 33100329
Species: Human
Sample Types: Cell Culture Supernates

Gao Y et al. (2020-08-06), 'Psoriasis 1' reduces Tâ€‘lymphocyteâ€‘mediated inflammation in patients with psoriasis by inhibiting vitamin D receptorâ€‘mediated STAT4 inactivation. Int J Mol Med, 2020-046(4):1538-1550.
PMID: 32945358
Species: Human
Sample Types: Plasma

HH Park et al. (2020-07-31), Acetylated K676 TGFBIp as a severity diagnostic blood biomarker for SARS-CoV-2 pneumonia Sci Adv, 2020-06(31).
PMID: 32937590
Species: Human
Sample Types: BALF

R Li et al. (2020-07-28), Preconditioning mesenchymal stromal cells with flagellin enhances the anti?inflammatory ability of their secretome against lipopolysaccharide?induced acute lung injury Molecular Medicine Reports, 2020-00(0).
PMID: 32945411
Species: Human
Sample Types: cell culture supernatant

HJ Hurley et al. (2020-07-08), Frontline Science: AMPK regulates metabolic reprogramming necessary for interferon production in human plasmacytoid dendritic cells J. Leukoc. Biol., 2020-00(0).
PMID: 32640499
Species: Human
Sample Types: Cell Culture Supernates

P Innamarato et al. (2020-06-24), Reactive Myelopoiesis Triggered by Lymphodepleting Chemotherapy Limits the Efficacy of Adoptive T Cell Therapy Mol. Ther., 2020-00(0).
PMID: 32615068
Species: Human
Sample Types: Plasma

M Tomaszewsk et al. (2020-06-24), Overexpression of PD-1 on Peripheral Blood Lymphocytes in Patients with Idiopathic Pulmonary Arterial Hypertension and Its Association with High Viral Loads of Epstein-Barr Virus and Poor Clinical Parameters J Clin Med, 2020-09(6).
PMID: 32599687
Species: Human
Sample Types: Cell Culture Supernates

T Zhou et al. (2020-06-24), IL-18BP is a secreted immune checkpoint and barrier to IL-18 immunotherapy Nature, 2020-00(0).
PMID: 32581358
Species: Human
Sample Types: Plasma

B Pedraz-Pet et al. (2020-06-11), Pro-inflammatory markers and fatigue in patients with depression: A case-control study Sci Rep, 2020-010(1):9494.
PMID: 32528052
Species: Human
Sample Types: Plasma

M Herrera et al. (2020-04-21), Gaps in Study Design for Immune Parameter Research for Latent Tuberculosis Infection: A Systematic Review J Immunol Res, 2020-02020(0):8074183.
PMID: 32377537
Species: Human
Sample Types: Cell Culture Supernates

C Dextras et al. (2020-03-30), Identification of Small Molecule Enhancers of Immunotherapy for Melanoma Sci Rep, 2020-010(1):5688.
PMID: 32231230
Species: Mouse
Sample Types: Cell Culture Supernates

Z Hu et al. (2020-03-26), PCC0208025 (BMS202), a small molecule inhibitor of PD-L1, produces an antitumor effect in B16-F10 melanoma-bearing mice PLoS ONE, 2020-015(3):e0228339.
PMID: 32214351
Species: Human
Sample Types: Cell Culture Supernates

AE El-Kadiry et al. (2020-03-12), A Novel Sulfonyl-Based Small Molecule Exhibiting Anti-cancer Properties Front Pharmacol, 2020-011(0):237.
PMID: 32231565
Species: Human
Sample Types: Cell Culture Supernates

N Sumransub et al. (2020-01-23), Breast cancer stem cell RNA-pulsed dendritic cells enhance tumor cell killing by effector T cells Oncol Lett, 2020-019(3):2422-2430.
PMID: 32194742
Species: Human
Sample Types: Serum

AM Moro et al. (2019-12-19), Evaluation of immunological, inflammatory, and oxidative stress biomarkers in gasoline station attendants BMC Pharmacol Toxicol, 2019-120(0):75.
PMID: 31852532
Species: Human
Sample Types: Cell Culture Supernates

BB Hofmann et al. (2019-12-18), N-Octanoyl-Dopamine inhibits cytokine production in activated T-cells and diminishes MHC-class-II expression as well as adhesion molecules in IFNgamma-stimulated endothelial cells Sci Rep, 2019-19(1):19338.
PMID: 31853095
Species: Human
Sample Types: Plasma

G Prencipe et al. (2019-12-17), The interferon-gamma pathway is selectively up-regulated in the liver of patients with secondary hemophagocytic lymphohistiocytosis PLoS ONE, 2019-114(12):e0226043.
PMID: 31846457
Species: Human
Sample Types: Cell Culture Supernates

E Terzuoli et al. (2019-12-06), ALDH3A1 Overexpression in Melanoma and Lung Tumors Drives Cancer Stem Cell Expansion, Impairing Immune Surveillance through Enhanced PD-L1 Output Cancers (Basel), 2019-111(12).
PMID: 31817719
Species: Human
Sample Types: Cell Culture Supernates

Z Mei et al. (2019-12-04), MUC1 as a target for CAR-T therapy in head and neck squamous cell carinoma Cancer Med, 2019-10(0).
PMID: 31800160
Species: Human
Sample Types: Cell Culture Supernates

MJ Kim et al. (2019-10-22), Structure establishment of three-dimensional (3D) cell culture printing model for bladder cancer PLoS ONE, 2019-114(10):e0223689.
PMID: 31639124
Species: Human
Sample Types: Plasma

IS Ribeiro et al. (2019-09-10), Association between body composition and inflammation: A central role of IL-17 and IL-10 in diabetic and hypertensive elderly women Exp. Gerontol., 2019-0127(0):110734.
PMID: 31518664
Species: Human
Sample Types: Serum

J Doll et al. (2019-08-13), LIPUS vs. reaming in non-union treatment: Cytokine expression course as a tool for evaluation and differentiation of non-union therapy J Orthop, 2019-017(0):208-214.
PMID: 31889743
Species: Human
Sample Types: Cell Culture Supernates

K Tashiro et al. (2019-06-25), Hepcidin exerts a negative immunological effect in pulmonary tuberculosis without HIV co-infection, prolonging the time to culture negative Int. J. Infect. Dis., 2019-086(0):47-54.
PMID: 31252187
Species: Human
Sample Types: Cell Culture Supernates

H Yu et al. (2019-06-17), Mesangial Cells Exhibit Features of Antigen-Presenting Cells and Activate CD4+ T Cell Responses J Immunol Res, 2019-02019(0):2121849.
PMID: 31317046
Species: Human
Sample Types: Cell Culture Supernates

DP Muñoz et al. (2019-06-11), Targetable mechanisms driving immunoevasion of persistent senescent cells link chemotherapy-resistant cancer to aging JCI Insight, 2019-05(0).
PMID: 31184599
Species: Human
Sample Types: Plasma

DE Martínez-F et al. (2019-04-30), Analysis of Genetic Variation in CD40 and CD40L: Relationship with mRNA Relative Expression and Soluble Proteins in Acute Coronary Syndrome J Immunol Res, 2019-02019(0):8063983.
PMID: 31183392
Species: Human
Sample Types: Cell Lysates

X Deng et al. (2019-04-01), Low-Dose Carbon Monoxide Inhibits Rhinovirus Replication in Human Alveolar and Airway Epithelial Cells Tohoku J. Exp. Med., 2019-0247(4):215-222.
PMID: 30971638
Species: Human
Sample Types: Plasma

S Clayton et al. (2019-02-11), Plasma levels of TNF-?, IL-6, IFN-?, IL-12, IL-17, IL-22, and IL-23 in achalasia, eosinophilic esophagitis (EoE), and gastroesophageal reflux disease (GERD) BMC Gastroenterol, 2019-019(1):28.
PMID: 30744559
Species: Human
Sample Types: Plasma

FN Osuji et al. (2018-12-03), The effects of highly active antiretroviral therapy on the serum levels of pro-inflammatory and anti-inflammatory cytokines in HIV infected subjects J. Biomed. Sci., 2018-125(1):88.
PMID: 30501642
Species: Human
Sample Types: Whole Cells

JH Kim et al. (2018-11-08), Effects of pepsin and pepstatin on reflux tonsil hypertrophy in vitro PLoS ONE, 2018-113(11):e0207090.
PMID: 30408092
Species: Human
Sample Types: Cell Culture Supernates

Wang J et al. (2018-10-10), VSIG-3 as a ligand of VISTA inhibits human T-cell function. Immunology, 2018-1156(1):74-85.
PMID: 30220083
Species: Human
Sample Types: Cell Culture Supernates

J Park et al. (2018-06-27), A 3D human triculture system modeling neurodegeneration and neuroinflammation in Alzheimer's disease Nat. Neurosci., 2018-021(7):941-951.
PMID: 29950669
Species: Mouse
Sample Types: Serum

Y Kagoya et al. (2018-05-15), DOT1L inhibition attenuates graft-versus-host disease by allogeneic T cells in adoptive immunotherapy models Nat Commun, 2018-09(1):1915.
PMID: 29765028
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

ZM Bian et al. (2018-02-16), Distinct CD40L receptors mediate inflammasome activation and secretion of IL-1? and MCP-1 in cultured human retinal pigment epithelial cells Exp. Eye Res., 2018-0170(0):29-39.
PMID: 29454857
Species: Human
Sample Types: Serum
Applications: ELISA Capture

G Jordakieva et al. (2018-02-03), Occupational exposure to ultrafine particles in police officers: no evidence for adverse respiratory effects J Occup Med Toxicol, 2018-013(0):5.
PMID: 29434649
Species: Human
Sample Types: Cell Culture Supernates

A Stiff et al. (2018-01-23), Nitric Oxide Production by Myeloid Derived Suppressor Cells Plays a Role in Impairing Fc Receptor-Mediated Natural Killer Cell Function Clin. Cancer Res., 2018-00(0).
PMID: 29363526
Species: Human
Sample Types: Cell Culture Supernates

M Najar et al. (2018-01-19), Mesenchymal stromal cells of the bone marrow and natural killer cells: cell interactions and cross modulation J Cell Commun Signal, 2018-00(0).
PMID: 29350342
Species: Human
Sample Types: Cell Culture Supernates

X Liu et al. (2018-01-01), Construction, Expression, and Characterization of rSEA-EGF and In Vitro Evaluation of its Antitumor Activity Against Nasopharyngeal Cancer Technol. Cancer Res. Treat., 2018-017(0):1533033818762.
PMID: 29551087
Species: Human
Sample Types: Plasma

AS Sampaio et al. (2018-01-01), Social conditions and immune response in human immunodeficiency virus-seropositive pregnant women: a cross-sectional study in Brazil Rev. Soc. Bras. Med. Trop., 2018-051(1):21-29.
PMID: 29513838
Species: Human
Sample Types: Serum

G Schmalz et al. (2017-09-04), MMP-8 and TIMP-1 are associated to periodontal inflammation in patients with rheumatoid arthritis under methotrexate immunosuppression - First results of a cross-sectional study J Microbiol Immunol Infect, 2017-00(0).
PMID: 28919284
Species: Bovine, Human
Sample Types: Cell Culture Supernates

A Ch Stratak et al. (2017-08-31), The in vitro and ex vivo effect of Auranta 3001 in preventing Cryptosporidium hominis and Cryptosporidium parvum infection Gut Pathog, 2017-09(0):49.
PMID: 28883891
Species: Human
Sample Types: Cell Culture Supernates

U Bughani et al. (2017-07-03), T cell activation and differentiation is modulated by a CD6 domain 1 antibody Itolizumab PLoS ONE, 2017-012(7):e0180088.
PMID: 28672038
Species: Human
Sample Types: Plasma

VR Ribeiro et al. (2017-06-19), Association between cytokine profile and transcription factors produced by T cells subsets in early and late-onset preeclampsia Immunology, 2017-00(0).
PMID: 28502089
Species: Human
Sample Types: Cell Culture Supernates

K Gonda et al. (2017-06-02), Myeloid-derived suppressor cells are increased and correlated with type 2 immune responses, malnutrition, inflammation, and poor prognosis in patients with breast cancer Oncol Lett, 2017-014(2):1766-1774.
PMID: 28789407
Species: Human
Sample Types: Cell Culture Supernates

T Yoshioka et al. (2017-03-22), Frequency and role of NKp46 and NKG2A in hepatitis B virus infection PLoS ONE, 2017-012(3):e0174103.
PMID: 28328926
Species: Human
Sample Types: Cell Culture Supernates

KC Clark et al. (2017-03-20), Human and feline adipose-derived mesenchymal stem cells have comparable phenotype, immunomodulatory functions, and transcriptome Stem Cell Res Ther, 2017-08(1):69.
PMID: 28320483
Species: Human
Sample Types: Cell Culture Supernates

J Liu et al. (2017-02-21), Upregulated unique long 16 binding protein 1 detected in preeclamptic placenta affects human extravillous trophoblast cell line (HTR-8/SVneo) invasion by modulating the function of uterine natural killer cells Exp Ther Med, 2017-013(4):1447-1455.
PMID: 28413491
Species: Human
Sample Types: Cell Culture Supernates

Yadollah Omidi (2016-11-06), Development of screen-printed tryptophan-kynurenine immunosensor for in vitro assay of kynurenine-mediated immunosuppression effect of cancer cells on activated T-cells Biosens Bioelectron, 2016-10(0).
PMID: 27840038
Species: Human
Sample Types: Cell Culture Supernates

Farrukh T Awan (2016-09-09), Therapeutic CD94/NKG2A blockade improves natural killer cell dysfunction in chronic lymphocytic leukemia Oncoimmunology, 2016-05(10):e1226720.
PMID: 27853650
Species: Human
Sample Types: Serum

(2016-08-10), Involvement of NK Cells and NKp30 Pathway in Antisynthetase Syndrome J Immunol, 2016-0197(5):1621-30.
PMID: 27511738
Species: Human
Sample Types: Serum

(2016-08-01), Alterations in the Fecal Microbiota of Patients with HIV-1 Infection: An Observational Study in A Chinese Population Sci Rep, 2016-06(0):30673.
PMID: 27477587
Species: Human
Sample Types: Tissue Homogenates

Li W et al. (2016-01-01), CCL20-CCR6 Cytokine Network Facilitate Treg Activity in Advanced Grades and Metastatic Variants of Hepatocellular Carcinoma. Scand J Immunol, 2016-083(1):33-7.
PMID: 26331965
Species: Mouse
Sample Types: Cell Culture Supernates

Zhao D et al. (2015-12-10), WNT5A transforms intestinal CD8alphaalpha(+) IELs into an unconventional phenotype with pro-inflammatory features. BMC Gastroenterol, 2015-115(0):173.
PMID: 26652024
Species: Human
Sample Types: Cell Culture Supernates

Valton J et al. (2015-06-10), A Multidrug-resistant Engineered CAR T Cell for Allogeneic Combination Immunotherapy. Mol Ther, 2015-023(9):1507-18.
PMID: 26061646
Species: Human
Sample Types: Plasma

Mankad D et al. (2015-03-21), A randomized, placebo controlled trial of omega-3 fatty acids in the treatment of young children with autism. Mol Autism, 2015-06(0):18.
PMID: 25798215
Species: Human
Sample Types: Whole Cells

Hagen J et al. (2015-02-11), Comparative Multi-Donor Study of IFNgamma Secretion and Expression by Human PBMCs Using ELISPOT Side-by-Side with ELISA and Flow Cytometry Assays. Cells, 2015-04(1):84-95.
PMID: 25679284
Species: Human
Sample Types: Serum

Zhao R et al. (2014-12-10), Exogenous IFN-beta regulates the RANKL-c-Fos-IFN-beta signaling pathway in the collagen antibody-induced arthritis model. J Transl Med, 2014-112(1):330.
PMID: 25491303
Species: Human
Sample Types: Plasma

He Y et al. (2014-08-21), Psychological stress exerts effects on pathogenesis of hepatitis B via type-1/type-2 cytokines shift toward type-2 cytokine response. PLoS ONE, 2014-09(8):e105530.
PMID: 25144199
Species: Human
Sample Types: Serum

Han L et al. (2014-07-17), Role of programmed death ligands in effective T-cell interactions in extranodal natural killer/T-cell lymphoma. Oncol Lett, 2014-08(4):1461-1469.
PMID: 25202350
Species: Human
Sample Types: Cell Culture Supernates

Schiavoni I et al. (2014-06-26), Live attenuated B. pertussis BPZE1 rescues the immune functions of Respiratory Syncytial virus infected human dendritic cells by promoting Th1/Th17 responses. PLoS ONE, 2014-09(6):e100166.
PMID: 24967823
Species: Human
Sample Types: Cell Culture Supernates

Zhang Y et al. (2014-06-16), Expression and functional characterization of NOD2 in decidual stromal cells isolated during the first trimester of pregnancy. PLoS ONE, 2014-09(6):e99612.
PMID: 24932916
Species: Human
Sample Types: Cell Culture Supernates

Huang W et al. (2014-04-01), EBV-encoded miR-BART20-5p and miR-BART8 inhibit the IFN-gamma-STAT1 pathway associated with disease progression in nasal NK-cell lymphoma. Am J Pathol, 2014-0184(4):1185-97.
PMID: 24655378
Species: Human
Sample Types: Cell Culture Supernates

Biancheri P et al. (2014-02-01), Absence of a role for interleukin-13 in inflammatory bowel disease. Eur J Immunol, 2014-044(2):370-85.
PMID: 24338958
Species: Human
Sample Types: Cell Culture Supernates

Lutay N et al. (2014-01-28), Mycobacteria bypass mucosal NF-kB signalling to induce an epithelial anti-inflammatory IL-22 and IL-10 response. PLoS ONE, 2014-09(1):e86466.
PMID: 24489729
Species: Human
Sample Types: Cell Culture Supernates

Krishnan G et al. (2014-01-11), Endocannabinoids affect innate immunity of Muller glia during HIV-1 Tat cytotoxicity. Mol Cell Neurosci, 2014-059(0):10-23.
PMID: 24418364
Species: Human
Sample Types: Cell Culture Supernates

Pliyev B et al. (2013-12-16), Neutrophil microparticles modulate cytokine production by natural killer cells. Cytokine, 2013-165(2):126-9.
PMID: 24355430
Species: Human
Sample Types: Plasma

Weng T et al. (2013-11-13), Effects of interval and continuous exercise training on CD4 lymphocyte apoptotic and autophagic responses to hypoxic stress in sedentary men. PLoS ONE, 2013-18(11):e80248.
PMID: 24236174
Species: Human
Sample Types: Cell Culture Supernates

Ciaglia E et al. (2013-07-11), N6-isopentenyladenosine, an endogenous isoprenoid end product, directly affects cytotoxic and regulatory functions of human NK cells through FDPS modulation. J Leukoc Biol, 2013-094(6):1207-19.
PMID: 23847096
Species: Human
Sample Types: Cell Culture Supernates

Vincenzi F et al. (2013-06-03), Multiple sclerosis lymphocytes upregulate A2A adenosine receptors that are antiinflammatory when stimulated. Eur J Immunol, 2013-043(8):2206-16.
PMID: 23661562
Species: Human
Sample Types: Serum

Johnston A et al. (2013-01-31), Susceptibility-associated genetic variation at IL12B enhances Th1 polarization in psoriasis. Hum Mol Genet, 2013-022(9):1807-15.
PMID: 23376980
Species: Human
Sample Types: Cell Culture Supernates

Toscano F et al. (2012-12-19), Cleaved/associated TLR3 represents the primary form of the signaling receptor. J Immunol, 2012-1190(2):764-73.
PMID: 23255358
Species: Human
Sample Types: Serum

Garcia-Bermudez M et al. (2012-10-15), Analysis of the Interferon Gamma (rs2430561, +874T/A) Functional Gene Variant in Relation to the Presence of Cardiovascular Events in Rheumatoid Arthritis. PLoS ONE, 2012-17(10):e47166.
PMID: 23077565
Species: Human
Sample Types: Serum

Kuriyama T et al. (2012-09-18), Engulfment of hematopoietic stem cells caused by down-regulation of CD47 is critical in the pathogenesis of hemophagocytic lymphohistiocytosis. Blood, 2012-0120(19):4058-67.
PMID: 22990013
Species: Human
Sample Types: Serum

Churchman SM et al. (2012-08-29), Multiplexing immunoassays for cytokine detection in the serum of patients with rheumatoid arthritis: lack of sensitivity and interference by rheumatoid factor. Clin. Exp. Rheumatol., 2012-030(4):534-42.
PMID: 22704737
Species: Human
Sample Types: Cell Culture Supernates

Shukla N et al. (2012-08-28), Potent adjuvanticity of a pure TLR7-agonistic imidazoquinoline dendrimer. PLoS ONE, 2012-07(8):e43612.
PMID: 22952720
Species: Human
Sample Types: Cell Culture Supernates

Pandya AD et al. (2011-10-21), Identification of human NK17/NK1 cells. PLoS ONE, 2011-16(10):e26780.
PMID: 22039549
Species: Human
Sample Types: Plasma

Lee N et al. (2011-10-13), Cytokine Response Patterns in Severe Pandemic 2009 H1N1 and Seasonal Influenza among Hospitalized Adults. PLoS ONE, 2011-16(10):e26050.
PMID: 22022504
Species: Human
Sample Types: Cell Culture Supernates

Sun J et al. (2011-09-13), Origination of new immunological functions in the costimulatory molecule B7-H3: the role of exon duplication in evolution of the immune system. PLoS ONE, 2011-06(9):e24751.
PMID: 21931843
Species: Human
Sample Types: Serum

Takei S et al. (2011-03-24), Soluble interleukin-18 receptor complex is a novel biomarker in rheumatoid arthritis. Arthritis Res. Ther., 2011-013(2):R52.
PMID: 21435242
Species: Human
Sample Types: Whole Tissue

Mulder DJ et al. (2011-02-01), Antigen presentation and MHC class II expression by human esophageal epithelial cells: role in eosinophilic esophagitis. Am. J. Pathol., 2011-0178(2):744-53.
PMID: 21281807
Species: Human
Sample Types: Tears

Jun AS et al. (2011-01-27), Subnormal cytokine profile in the tear fluid of keratoconus patients. PLoS ONE, 2011-06(1):e16437.
PMID: 21298010
Species: Human
Sample Types: Cell Culture Supernates

Brandau S et al. (2010-11-24), Myeloid-derived suppressor cells in the peripheral blood of cancer patients contain a subset of immature neutrophils with impaired migratory properties. J. Leukoc. Biol., 2010-189(2):311-7.
PMID: 21106641
Species: Human
Sample Types: Cell Culture Supernates

Song W et al. (2010-11-19), HDAC inhibition by LBH589 affects the phenotype and function of human myeloid dendritic cells. Leukemia, 2010-125(1):161-8.
PMID: 21102427
Species: Human
Sample Types: Tissue Homogenates

Bachert C et al. (2010-11-01), Presence of IL-5 protein and IgE antibodies to staphylococcal enterotoxins in nasal polyps is associated with comorbid asthma. J. Allergy Clin. Immunol., 2010-1126(5):962.
PMID: 20810157
Species: Human
Sample Types: Serum

Antonelli A et al. (2010-10-07), Interleukin-1beta, C-x-C motif ligand 10, and interferon-gamma serum levels in mixed cryoglobulinemia with or without autoimmune thyroiditis. J. Interferon Cytokine Res., 2010-130(11):835-42.
PMID: 20929277
Species: Human
Sample Types: Cell Culture Supernates

Zahn S et al. (2010-08-19), Evidence for a pathophysiological role of keratinocyte-derived type III interferon (IFN-lambda) in cutaneous lupus erythematosus. J. Invest. Dermatol., 2010-0131(1):133-40.
PMID: 20720564
Species: Human
Sample Types: Plasma

Zhang G et al. (2010-08-09), B7-h3 augments the inflammatory response and is associated with human sepsis. J. Immunol., 2010-0185(6):3677-84.
PMID: 20696859
Species: Human
Sample Types: Cell Culture Supernates

Beziat V et al. (2010-02-13), Shaping of iNKT cell repertoire after unrelated cord blood transplantation. Clin. Immunol., 2010-0135(3):364-73.
PMID: 20153980
Species: Human
Sample Types: Plasma

Rutanen J et al. (2010-01-27), SIRT1 mRNA expression may be associated with energy expenditure and insulin sensitivity. Diabetes, 2010-059(4):829-35.
PMID: 20107110
Species: Human
Sample Types: Serum

Reeves EP et al. (2009-12-21), IL-8 dictates glycosaminoglycan binding and stability of IL-18 in cystic fibrosis. J. Immunol., 2009-1184(3):1642-52.
PMID: 20026745
Species: Human
Sample Types: Cell Culture Supernates

Ogbomo H et al. (2009-08-07), A novel immunomodulatory mechanism of ribavirin in suppressing natural killer cell function. Biochem. Pharmacol., 2009-079(2):188-97.
PMID: 19665449
Species: Human
Sample Types: Cell Culture Supernates

Oh JY et al. (2009-02-14), Cytokine secretion by human mesenchymal stem cells cocultured with damaged corneal epithelial cells. Cytokine, 2009-046(1):100-3.
PMID: 19223198
Species: Human
Sample Types: Plasma

Rodriguez PC et al. (2009-02-05), Arginase I-producing myeloid-derived suppressor cells in renal cell carcinoma are a subpopulation of activated granulocytes. Cancer Res., 2009-069(4):1553-60.
PMID: 19201693
Species: Human
Sample Types: Cell Culture Supernates

Mabilleau G et al. (2009-01-12), Interleukin-32 promotes osteoclast differentiation but not osteoclast activation. PLoS ONE, 2009-04(1):e4173.
PMID: 19137064
Species: Human
Sample Types: Cell Culture Supernates

Eyerich K et al. (2008-12-03), IL-17 in atopic eczema: linking allergen-specific adaptive and microbial-triggered innate immune response. J. Allergy Clin. Immunol., 2008-1123(1):59-66.e4.
PMID: 19056110
Species: Human
Sample Types: BALF

Hartl D et al. (2008-12-01), Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases. J. Immunol., 2008-1181(11):8053-67.
PMID: 19017998
Species: Human
Sample Types: Nasal Lavage Fluid

Niederfuhr A et al. (2008-10-01), Staphylococcus aureus in nasal lavage and biopsy of patients with chronic rhinosinusitis. Allergy, 2008-163(10):1359-67.
PMID: 18782116
Species: Human
Sample Types: Cell Culture Supernates

Yokoi T et al. (2008-08-13), Mycobacterium bovis Bacillus Calmette-Guerin suppresses inflammatory Th2 responses by inducing functional alteration of TSLP-activated dendritic cells. Int. Immunol., 2008-020(10):1321-9.
PMID: 18703465
Species: Human
Sample Types: Cell Culture Supernates

Djoba Siawaya JF et al. (2008-07-02), An evaluation of commercial fluorescent bead-based luminex cytokine assays. PLoS ONE, 2008-03(7):e2535.
PMID: 18596971
Species: Human
Sample Types: Cell Culture Supernates

Liu TY et al. (2008-04-01), Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells. Eur. J. Immunol., 2008-038(4):1012-23.
PMID: 18350544
Species: Human
Sample Types: Plasma

Stefanescu R et al. (2008-03-13), Synergistic interactions between interferon-gamma and TRAIL modulate c-FLIP in endothelial cells, mediating their lineage-specific sensitivity to thrombotic thrombocytopenic purpura plasma-associated apoptosis. Blood, 2008-0112(2):340-9.
PMID: 18339897
Species: Human
Sample Types: Cell Culture Supernates

Petermann KB et al. (2007-12-01), CD200 is induced by ERK and is a potential therapeutic target in melanoma. J. Clin. Invest., 2007-1117(12):3922-3929.
PMID: 18008004
Species: Human
Sample Types: Cell Culture Supernates

Mian MF et al. (2007-11-30), Impairment of human NK cell cytotoxic activity and cytokine release by cigarette smoke. J. Leukoc. Biol., 2007-183(3):774-84.
PMID: 18055568
Species: Human
Sample Types: Cell Culture Supernates

Vidyarani M et al. (2007-10-29), 1, 25 Dihydroxyvitamin D3 modulated cytokine response in pulmonary tuberculosis. Cytokine, 2007-140(2):128-34.
PMID: 17911026
Species: Human
Sample Types: Serum

Little RF et al. (2007-09-10), Phase 2 study of pegylated liposomal doxorubicin in combination with interleukin-12 for AIDS-related Kaposi sarcoma. Blood, 2007-0110(13):4165-71.
PMID: 17846226
Species: Human
Sample Types: Serum

Frances R et al. (2007-09-05), Translocation of bacterial DNA from Gram-positive microorganisms is associated with a species-specific inflammatory response in serum and ascitic fluid of patients with cirrhosis. Clin. Exp. Immunol., 2007-0150(2):230-7.
PMID: 17822441
Species: Human
Sample Types: Cell Culture Supernates

Lin YL et al. (2007-08-30), Placental growth factor down-regulates type 1 T helper immune response by modulating the function of dendritic cells. J. Leukoc. Biol., 2007-082(6):1473-80.
PMID: 17761954
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Plasma

Clay CC et al. (2007-08-22), Neuroinvasion of fluorescein-positive monocytes in acute simian immunodeficiency virus infection. J. Virol., 2007-081(21):12040-8.
PMID: 17715237
Species: Human
Sample Types: Serum

Seeber B et al. (2007-08-13), Panel of markers can accurately predict endometriosis in a subset of patients. Fertil. Steril., 2007-089(5):1073-81.
PMID: 17706208
Species: Human
Sample Types: Plasma

Irhimeh MR et al. (2007-06-01), Fucoidan ingestion increases the expression of CXCR4 on human CD34+ cells. Exp. Hematol., 2007-035(6):989-94.
PMID: 17533053
Species: Human
Sample Types: Cell Culture Supernates

Wolk K et al. (2007-05-01), IL-22 induces lipopolysaccharide-binding protein in hepatocytes: a potential systemic role of IL-22 in Crohn's disease. J. Immunol., 2007-0178(9):5973-81.
PMID: 17442982
Species: Human
Sample Types: Serum

Lee MT et al. (2007-01-01), Interferon-beta and adhesion molecules (E-selectin and s-intracellular adhesion molecule-1) are detected in sera from patients with retinal vasculitis and are induced in retinal vascular endothelial cells by Toll-like receptor 3 signalling. Clin. Exp. Immunol., 2007-0147(1):71-80.
PMID: 17177965
Species: Human
Sample Types: Cell Culture Supernates

Ebert EC et al. (2006-08-17), Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription. Cell. Immunol., 2006-0241(1):7-13.
PMID: 16914126
Species: Human
Sample Types: Cell Culture Supernates

Karrar A et al. (2006-07-13), Human liver sinusoidal endothelial cells induce apoptosis in activated T cells: a role in tolerance induction. Gut, 2006-056(2):243-52.
PMID: 16840505
Species: Human
Sample Types: Cell Culture Supernates

Perryman LA et al. (2006-05-11), Over-expression of p53 mutants in LNCaP cells alters tumor growth and angiogenesis in vivo. Biochem. Biophys. Res. Commun., 2006-0345(3):1207-14.
PMID: 16723121
Species: Human
Sample Types: Plasma

Wolk K et al. (2006-05-01), IL-22 regulates the expression of genes responsible for antimicrobial defense, cellular differentiation, and mobility in keratinocytes: a potential role in psoriasis. Eur. J. Immunol., 2006-036(5):1309-23.
PMID: 16619290
Species: Human
Sample Types: Cell Culture Supernates

Skoberne M et al. (2006-04-13), The apoptotic-cell receptor CR3, but not alphavbeta5, is a regulator of human dendritic-cell immunostimulatory function. Blood, 2006-0108(3):947-55.
PMID: 16614246
Species: Human
Sample Types: Plasma

Claessens YE et al. (2006-03-30), Erythropoiesis abnormalities contribute to early-onset anemia in patients with septic shock. Am. J. Respir. Crit. Care Med., 2006-0174(1):51-7.
PMID: 16574939
Species: Human
Sample Types: Serum

Yoshikawa H et al. (2006-03-29), Elevation of IL-12 p40 and its antibody in myasthenia gravis with thymoma. J. Neuroimmunol., 2006-0175(1):169-75.
PMID: 16574246
Species: Human
Sample Types: Serum

Hartl D et al. (2006-03-16), Chemokines indicate allergic bronchopulmonary aspergillosis in patients with cystic fibrosis. Am. J. Respir. Crit. Care Med., 2006-0173(12):1370-6.
PMID: 16543550
Species: Human
Sample Types: Serum

Kiryushko D et al. (2006-02-02), Th2 cytokine response in Major Depressive Disorder patients before treatment. J. Neuroimmunol., 2006-0172(1):156-65.
PMID: 16457895
Species: Human
Sample Types: Plasma

Yamanaka K et al. (2006-01-15), Expression of interleukin-18 and caspase-1 in cutaneous T-cell lymphoma. Clin. Cancer Res., 2006-012(2):376-82.
PMID: 16428475
Species: Human
Sample Types: Cell Culture Supernates

Hanabuchi S et al. (2006-01-05), Human plasmacytoid predendritic cells activate NK cells through glucocorticoid-induced tumor necrosis factor receptor-ligand (GITRL). Blood, 2006-0107(9):3617-23.
PMID: 16397134
Species: Human
Sample Types: Cell Culture Supernates

von Strandmann EP et al. (2005-10-06), A novel bispecific protein (ULBP2-BB4) targeting the NKG2D receptor on natural killer (NK) cells and CD138 activates NK cells and has potent antitumor activity against human multiple myeloma in vitro and in vivo. Blood, 2005-1107(5):1955-62.
PMID: 16210338
Species: Human
Sample Types: Tissue Secretion

Kristjansson S et al. (2005-10-01), Respiratory syncytial virus and other respiratory viruses during the first 3 months of life promote a local TH2-like response. J. Allergy Clin. Immunol., 2005-1116(4):805-11.
PMID: 16210054
Species: Human
Sample Types: Cell Culture Supernates

Takei M et al. (2005-05-01), Effect of piceatannol in human monocyte-derived dendritic cells in vitro. J Pharm Sci, 2005-094(5):974-82.
PMID: 15793803
Species: Human
Sample Types: Serum

Huang JL et al. (2005-02-24), Th2 predominance and CD8+ memory T cell depletion in patients with severe acute respiratory syndrome. Microbes Infect., 2005-07(3):427-36.
PMID: 15784184
Species: Human
Sample Types: CSF

Mistchenko AS et al. (2005-02-01), Detection of interleukin 10 in cerebrospinal fluid of patients with subacute sclerosing panencephalitis. J. Neurovirol., 2005-011(1):66-9.
PMID: 15804961
Species: Human
Sample Types: Plasma

Jensen J et al. (2005-01-07), Cytokines and adhesion molecules in multiple sclerosis patients treated with interferon-beta1b. Cytokine, 2005-029(1):24-30.
PMID: 15579375
Species: Human
Sample Types: Cell Culture Supernates

Kahlon KS et al. (2004-12-15), Specific recognition and killing of glioblastoma multiforme by interleukin 13-zetakine redirected cytolytic T cells. Cancer Res., 2004-164(24):9160-6.
PMID: 15604287
Species: Human
Sample Types: Cell Culture Supernates

Saeki S et al. (2004-09-01), Effects of antiasthmatic agents on the functions of peripheral blood monocyte-derived dendritic cells from atopic patients. J. Allergy Clin. Immunol., 2004-0114(3):538-44.
PMID: 15356554
Species: Human
Sample Types: Cell Culture Supernates

Wolk K et al. (2004-08-01), IL-22 increases the innate immunity of tissues. Immunity, 2004-021(2):241-54.
PMID: 15308104
Species: Human
Sample Types: Serum

Alter P et al. (2004-07-01), Escape from cardiomyocyte apoptosis by enterovirus persistence due to elevated soluble Fas-receptors. Z Kardiol, 2004-093(7):524-32.
PMID: 15243763
Species: Human
Sample Types: Cell Culture Supernates

Fieschi C et al. (2004-06-03), A novel form of complete IL-12/IL-23 receptor beta1 deficiency with cell surface-expressed nonfunctional receptors. Blood, 2004-0104(7):2095-101.
PMID: 15178580
Species: Human
Sample Types: Tissue Secretion

Garofalo RP et al. (2004-06-01), Production of interferon gamma in respiratory syncytial virus infection of humans is not associated with interleukins 12 and 18. J. Med. Virol., 2004-073(2):289-94.
PMID: 15122806
Species: Human
Sample Types: Nasal Lavage Fluid

Ciprandi G et al. (2004-04-01), Cytokines evaluation in nasal lavage of allergic children after Bacillus clausii administration: a pilot study. Pediatr Allergy Immunol, 2004-015(2):148-51.
PMID: 15059191
Species: Human
Sample Types: Seminal Plasma

Basu S et al. (2004-03-01), Inflammatory cytokine concentrations are elevated in seminal plasma of men with spinal cord injuries. J. Androl., 2004-025(2):250-4.
PMID: 14760011
Species: Human
Sample Types: Cell Culture Supernates

Durali D et al. (2003-07-31), In human B cells, IL-12 triggers a cascade of molecular events similar to Th1 commitment. Blood, 2003-0102(12):4084-9.
PMID: 12893768
Species: Human
Sample Types: Cell Culture Supernates

Wu C et al. (2003-06-01), IL-18 receptor beta-induced changes in the presentation of IL-18 binding sites affect ligand binding and signal transduction. J. Immunol., 2003-0170(11):5571-7.
PMID: 12759435
Species: Human
Sample Types: Cell Culture Supernates

Giunti D et al. (2003-05-01), Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS. J. Leukoc. Biol., 2003-073(5):584-90.
PMID: 12714572
Species: Human
Sample Types: Cell Culture Supernates

D&apos;Ambrosio D (2002-10-01), Skewing of cytotoxic activity and chemokine production, but not of chemokine receptor expression, in human type-1/-2 gamma delta T lymphocytes. Eur. J. Immunol., 2002-132(10):2934-43.
PMID: 12355447
Species: Human
Sample Types: Ascites Fluid

Marme A et al. (2002-09-10), Intraperitoneal bispecific antibody (HEA125xOKT3) therapy inhibits malignant ascites production in advanced ovarian carcinoma. Int. J. Cancer, 2002-0101(2):183-9.
PMID: 12209996
Species: Human
Sample Types: Cell Culture Supernates

Lazetic S et al. (2002-08-06), Chimeric co-stimulatory molecules that selectively act through CD28 or CTLA-4 on human T cells. J. Biol. Chem., 2002-0277(41):38660-8.
PMID: 12167647
Species: Human
Sample Types: Cell Culture Supernates

Parham C et al. (2002-06-01), A receptor for the heterodimeric cytokine IL-23 is composed of IL-12Rbeta1 and a novel cytokine receptor subunit, IL-23R. J. Immunol., 2002-0168(11):5699-708.
PMID: 12023369
Species: Human
Sample Types: Cell Culture Supernates

Briard D et al. (2002-05-01), Fibroblasts from human spleen regulate NK cell differentiation from blood CD34(+) progenitors via cell surface IL-15. J. Immunol., 2002-0168(9):4326-32.
PMID: 11970974
Species: Human
Sample Types: Blister Fluid

Baroni A et al. (2002-01-01), Cytokine pattern in blister fluid and sera of patients with pemphigus. Dermatology (Basel), 2002-0205(2):116-21.
PMID: 12218224
Species: Human
Sample Types: Hydrosalpinx Fluid

Chen CD et al. (2002-01-01), The significance of cytokines, chemical composition, and murine embryo development in hydrosalpinx fluid for predicting the IVF outcome in women with hydrosalpinx. Hum. Reprod., 2002-017(1):128-33.
PMID: 11756375
Species: Human
Sample Types: Serum

Hamzaoui K et al. (2002-01-01), Cytokine profile in Behcet's disease patients. Relationship with disease activity. Scand. J. Rheumatol., 2002-031(4):205-10.
PMID: 12369651
Species: Human
Sample Types: Serum

Cotler SJ et al. (2001-12-01), An analysis of acute changes in interleukin-6 levels after treatment of hepatitis C with consensus interferon. J. Interferon Cytokine Res., 2001-121(12):1011-9.
PMID: 11798458
Species: Human

Jotwani R et al. (2001-10-15), Mature dendritic cells infiltrate the T cell-rich region of oral mucosa in chronic periodontitis: in situ, in vivo, and in vitro studies. 2001-1167(8):4693-700.
PMID: 11591800
Species: Human
Sample Types: Cell Culture Supernates

Albanesi C et al. (2001-10-01), A cytokine-to-chemokine axis between T lymphocytes and keratinocytes can favor Th1 cell accumulation in chronic inflammatory skin diseases. J. Leukoc. Biol., 2001-170(4):617-23.
PMID: 11590199
Species: Human
Sample Types: Cell Culture Supernates

Rigano R et al. (2001-01-01), Modulation of human immune response by Echinococcus granulosus antigen B and its possible role in evading host defenses. Infect. Immun., 2001-069(1):288-96.
PMID: 11119517
Species: Human
Sample Types: Cell Culture Supernates

Kadowaki N et al. (2000-07-17), Natural interferon alpha/beta-producing cells link innate and adaptive immunity. J. Exp. Med., 2000-0192(2):219-26.
PMID: 10899908
Species: Human
Sample Types: Saliva

Black et al. (2000-04-01), Cytokine profiles in parotid saliva from HIV-1-infected individuals: changes associated with opportunistic infections in the oral cavity. Oral Microbiol Immunol, 2000-015(2):74-81.
PMID: 11155169
Species: Human
Sample Types: Nasal Lavage Fluid

Benson M et al. (2000-02-01), Low levels of interferon-gamma in nasal fluid accompany raised levels of T-helper 2 cytokines in children with ongoing allergic rhinitis. Pediatr Allergy Immunol, 2000-011(1):20-8.
PMID: 10768732
Species: Human
Sample Types: Pleural Effusion

Chen YM et al. (2000-01-01), Restoration of cytotoxic T lymphocyte function in malignant pleural effusion: interleukin-15 vs. interleukin-2. J. Interferon Cytokine Res., 2000-020(1):31-9.
PMID: 10670650
Species: Primate
Sample Types: Serum

Yang C et al. (1999-08-01), Cytokine production in rhesus monkeys infected with Plasmodium coatneyi. Am. J. Trop. Med. Hyg., 1999-061(2):226-9.
PMID: 10463671

There are no citations that match your criteria.

Showing 1 - 10 of 205 citations Showing All

Customer Reviews for Human IFN-gamma Quantikine ELISA Kit (10)

4.8 out of 5

10 customer ratings

5 stars

80%

4 stars

20%

3 stars

2 stars

1 star

Customer Images

Showing 1 - 5 of 10 reviews Showing All

Filter By:

Human IFN-gamma Quantikine ELISA Kit

Name: Anonymous

Sample Tested: Cell culture supernatant

Verified Customer | Posted 02/27/2023
Human IFN-gamma Quantikine ELISA Kit

Name: Anonymous

Sample Tested: Plasma

Verified Customer | Posted 11/16/2022

Plasma tested at 2-fold dilution. Strong matrix effect observed for S6 & S7.
Human IFN-gamma Quantikine ELISA Kit

Name: Lu Bai

Sample Tested: Cell culture supernatant,human PBMC

Verified Customer | Posted 12/22/2021
Human IFN-gamma Quantikine ELISA Kit

Name: Anonymous

Sample Tested: human primary NK cells,Peripheral blood mononuclear cells (PBMCs)

Verified Customer | Posted 09/15/2021

PBMCs from healthy donors or isolated primary human NK cells were stimulated and tested for IFNg secretion. Following protocol, it was easy to obtain clean and consistent results between indpendent experiments.
Human IFN-gamma Quantikine ELISA Kit

Name: Anonymous

Sample Tested: HepG2 human hepatocellular carcinoma cell line,MDA-MB-231 human breast cancer cell line

Verified Customer | Posted 03/11/2021

Easy to use, protocol detailed and easy to follow. Reproducible standard curve.
Human IFN-gamma Quantikine ELISA Kit

Name: Anonymous

Sample Tested: Cancer Cells,T cells

Verified Customer | Posted 02/24/2021
Human IFN-gamma Quantikine ELISA Kit

Name: Anonymous

Sample Tested: HepG2 human hepatocellular carcinoma cell line,MDA-MB-231 human breast cancer cell line

Verified Customer | Posted 01/22/2021

The assay was easy to perform according to the supplied insctructions. We were satisfied with the standard curve, for the samples the right dilution still needs to be figured out.
Human IFN-gamma Quantikine ELISA Kit

Name: Anonymous

Sample Tested: Serum

Verified Customer | Posted 11/13/2020

Standards in duplicate in all the wells in 1 and 2.R&D QC Low in well 3AR&D QC Med in well 12GR&D QC High in well 12HPipetting error or washing error didnt develop the best standard curve however the rest worked great.
Human IFN-gamma Quantikine ELISA Kit

Name: Lindsie Morin

Sample Tested: Serum

Verified Customer | Posted 11/12/2020

Great protocol that was easy to follow. The curve developed very well with expected results.
Human IFN-gamma Quantikine ELISA Kit

Name: Anonymous

Sample Tested: EDTA Plasma

Verified Customer | Posted 11/12/2020

Excellent background detail that was relevant for understanding the assay, and easy to follow protocol.

There are no reviews that match your criteria.

Showing 1 - 5 of 10 reviews Showing All

FAQs for Human IFN-gamma Quantikine ELISA Kit

Assay Diluent RD1-63 contains visible precipitate. Is this acceptable to use?

A small amount of precipitate is expected and acceptable in Assay Diluent RD1-63. Variability has been observed between vials and diluent lots. It may not fully resolubilize but this will not impact assay performance. It is not recommended to filter the precipitate.

Human IFN-gamma Quantikine ELISA Kit Best Seller

Catalog # DIF50C | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Customers also Purchased

Product Specifications for Human IFN-gamma Quantikine ELISA Kit

Precision

Recovery

Linearity

Scientific Data Examples for Human IFN-gamma Quantikine ELISA Kit

Preparation and Storage

Background: IFN-gamma

Citations for Human IFN-gamma Quantikine ELISA Kit (205)

Customer Reviews for Human IFN-gamma Quantikine ELISA Kit (10)

Customer Images

Human IFN-gamma Quantikine ELISA Kit

Human IFN-gamma Quantikine ELISA Kit

Human IFN-gamma Quantikine ELISA Kit

Human IFN-gamma Quantikine ELISA Kit

Human IFN-gamma Quantikine ELISA Kit

Human IFN-gamma Quantikine ELISA Kit

Human IFN-gamma Quantikine ELISA Kit

Human IFN-gamma Quantikine ELISA Kit

Human IFN-gamma Quantikine ELISA Kit

Human IFN-gamma Quantikine ELISA Kit

FAQs for Human IFN-gamma Quantikine ELISA Kit

Product Documents for Human IFN-gamma Quantikine ELISA Kit

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne 品牌

保持关注