Human alpha-L-Iduronidase/IDUA ELISA Kit (Colorimetric)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-39933
Key Product Details
Sample Type & Volume Required Per Well
Tissue homogenates, cell lysates and other biological fluids (100 uL)
Sensitivity
0.63 ng/mL (example only; lot dependent)
Assay Range
1.56 - 100 ng/mL (example only; lot dependent)
Product Specifications
Assay Type
Sandwich ELISA
Kit Type
ELISA Kit (Colorimetric)
Reactivity
Human
Description
Assay Length: 4.5 hours
Precision
Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)
Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)
Scientific Data Images for Human alpha-L-Iduronidase/IDUA ELISA Kit (Colorimetric)
ELISA: Human alpha-L-Iduronidase/IDUA ELISA Kit (Colorimetric) [NBP3-39933]
ELISA: Human alpha-L-Iduronidase/IDUA ELISA Kit (Colorimetric) [NBP3-39933] - Standard Curve ReferenceKit Contents for Human alpha-L-Iduronidase/IDUA ELISA Kit (Colorimetric)
- Detection Reagent A
- Detection Reagent B
- Diluent Buffer
- Instruction manual
- Plate sealer for 96 wells
- Pre-coated 96T strip plate
- Standard
- Stop Solution
- TMB Substrate
- Wash Buffer (30 x concentrate)
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: alpha-L-Iduronidase/IDUA
alpha-L-Iduronidase, encoded by the IDUA gene, is an important enzyme required for the lysosomal degradation of glycosaminoglycans (GAGs). It hydrolyzes the non-reducing terminal a-L-iduronic acid residues in GAGs, including dermatan sulfate and heparan sulfate. Mutations in IDUA that result in enzymatic deficiency lead to the autosomal recessive disease mucopolysaccharidosis type I (MPS I). MPS I causes progressive cellular, tissue, and organ damage.
Alternate Names
alphaLIduronidase, IDA, IDUA
Gene Symbol
IDUA
Additional alpha-L-Iduronidase/IDUA Products
Product Documents for Human alpha-L-Iduronidase/IDUA ELISA Kit (Colorimetric)
Product Specific Notices for Human alpha-L-Iduronidase/IDUA ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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