Human monocyte-derived dendritic cells were stained with Vitamin D3 Monoclonal Antibody (Catalog # MAB6566, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Applications for Vitamin D3 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human monocyte-derived dendritic cells, fixed with paraformaldehyde and permeabilized with saponin

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Vitamin D3

25-hydroxy-vitamin D₃ (25(OH)D₃) is synthesized in the liver and is the primary circulating form of vitamin D. Its blood concentration, which reflects 25(OH)D₃ produced by exposure to ultraviolet B, as well as dietary and vitamin D supplementation, is felt to be the best indicator of vitamin D status. 25(OH)D₃ is metabolized to 1 alpha,25(OH)₂D₃ in the proximal tubular cells of the kidney by the enzyme 1 alpha-hydroxylase. The vitamin D structure is similar to that of classic steroid hormones, such as estradiol, cortisol, and aldosterone in that they have the same root cyclopentanoperhydrophenanthrene ring structure. It has been shown that the active steroid hormone 1 alpha,25(OH)₂D₃ is essential for life in higher animals. Besides playing important roles in calcium homeostasis and bone mineral metabolism, it is now known to play a role in cellular differentiation, inhibition of cell growth, immune regulation and the prevention of neoplastic transformation. The active form of vitamin D₃, 1 alpha,25(OH)₂D₃, acts both through its cellular receptor, the vitamin D receptor (VDR), and through other extrarenal targets in an autocrine and paracrine manner where 1 alpha-hydroxylase is present.

Alternate Names

Calcifediol, Calciol, Calcitriol, Cholecalciferol, VD3

Additional Vitamin D3 Products

Product Documents for Vitamin D3 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Vitamin D3 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Vitamin D3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6566

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Vitamin D3 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Vitamin D3 Antibody

Detection of Vitamin D3 in Human monocyte-derived dendritic cells by Flow Cytometry.