u-Plasminogen Activator/Urokinase Antibody (204212) [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # FAB1310CL1
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA Standard (Matched Pair), ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair), Immunoprecipitation
Label
CoraFluor 1
Antibody Source
Monoclonal Mouse IgG2A Clone # 204212
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human u-Plasminogen Activator (uPA)/Urokinase Catalytic Domain
Ser21-Leu431
Accession # P00749
Ser21-Leu431
Accession # P00749
Specificity
Detects human u-Plasminogen Activator (uPA)/Urokinase Catalytic Domain in direct ELISAs and Western blots. In Western blots under reducing conditions, it reacts with the catalytic domain (B chain) only. In Western blots under non-reducing conditions, it reacts with the B chain and disulfide bond-linked B and A chains. In direct ELISAs, no cross-reactivity with recombinant human (rh) Factor X, rhHGFA, rhKLK‑3, -4, -5, -6, -8, -10, -11, rhThrombin, or recombinant mouse WIF-1 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
CoraFluor(TM) 1, amine reactive
CoraFluor(TM) 1, thiol reactive
For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
CoraFluor(TM) 1, amine reactive
CoraFluor(TM) 1, thiol reactive
For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
Applications for u-Plasminogen Activator/Urokinase Antibody (204212) [CoraFluor™ 1]
Application
Recommended Usage
ELISA Capture (Matched Antibody Pair)
Optimal dilutions of this antibody should be experimentally determined.
ELISA Detection (Matched Antibody Pair)
Optimal dilutions of this antibody should be experimentally determined.
ELISA Standard (Matched Pair)
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: u-Plasminogen Activator (uPA)/Urokinase
Long Name
Urokinase-type Plasminogen Activator
Alternate Names
PLAU, uPA, uPlasminogen Activator, Urokinase
Gene Symbol
PLAU
Additional u-Plasminogen Activator (uPA)/Urokinase Products
- All Products for u-Plasminogen Activator (uPA)/Urokinase
- u-Plasminogen Activator (uPA)/Urokinase Activity Assays
- u-Plasminogen Activator (uPA)/Urokinase cDNA Clones
- u-Plasminogen Activator (uPA)/Urokinase ELISA Kits
- u-Plasminogen Activator (uPA)/Urokinase Lysates
- u-Plasminogen Activator (uPA)/Urokinase Primary Antibodies
- u-Plasminogen Activator (uPA)/Urokinase Proteins and Enzymes
- u-Plasminogen Activator (uPA)/Urokinase Simple Plex
Product Specific Notices for u-Plasminogen Activator/Urokinase Antibody (204212) [CoraFluor™ 1]
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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