RGM-B Antibody [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # AF3597CL1
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot, CyTOF-ready
Label
CoraFluor 1
Antibody Source
Polyclonal Sheep IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse RGM-B
Gly49-Ser414
Accession # Q7TQ33
Gly49-Ser414
Accession # Q7TQ33
Specificity
Detects mouse RGM-B in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse (rm) RGM-A and rmRGM-C is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
CoraFluor(TM) 1, amine reactive
CoraFluor(TM) 1, thiol reactive
For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
CoraFluor(TM) 1, amine reactive
CoraFluor(TM) 1, thiol reactive
For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
Applications for RGM-B Antibody [CoraFluor™ 1]
Application
Recommended Usage
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: RGM-B
The repulsive guidance molecule (RGM) family of molecules contains three uniquely structured GPI-linked glycoproteins that participate in a variety of activities. Termed RGM-A, -B and -C, they are 40-45 kDa in size, show generally nonoverlapping fetal expression patterns, and serve as BMP-4 receptors. RGM-A and -C are plasma membrane bound, while RGM-B is generally microsomal in location. RGM-A and -B are associated with neurons, while RGM-C regulates skeletal muscle and hepatocyte function.
Long Name
Repulsive Guidance Molecule B
Alternate Names
Dragon, RGMB
Gene Symbol
RGMB
Additional RGM-B Products
Product Documents for RGM-B Antibody [CoraFluor™ 1]
Product Specific Notices for RGM-B Antibody [CoraFluor™ 1]
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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