Ras Antibody (HL10) - G12D Mutant - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13647
Recombinant Monoclonal Antibody
![Western Blot: Ras Antibody (HL10)G12D Mutant [NBP3-13647]](https://resources.bio-techne.com/images/products/Ras-Antibody-HL10-G12D-Mutant-Western-Blot-NBP3-13647-img0004.jpg "Western Blot: Ras Antibody (HL10)G12D Mutant [NBP3-13647]")
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL10 expressed in HEK293
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide surrounding mutant G12D of human K-Ras. The exact sequence is proprietary.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Centrifuge briefly prior to opening.
Scientific Data Images for Ras Antibody (HL10) - G12D Mutant - Azide and BSA Free
![Western Blot: Ras Antibody (HL10)G12D Mutant [NBP3-13647]](https://resources.bio-techne.com/images/products/Ras-Antibody-HL10-G12D-Mutant-Western-Blot-NBP3-13647-img0005.jpg "Western Blot: Ras Antibody (HL10)G12D Mutant [NBP3-13647]")
Western Blot: Ras Antibody (HL10)G12D Mutant [NBP3-13647]
Western Blot: Ras Antibody (HL10) - G12D Mutant [NBP3-13647] - Non-transfected (-) and transfected (+) Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ras (G12D Mutant) antibody [HL10] (NBP3-13647) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.![Immunohistochemistry-Paraffin: Ras Antibody (HL10) - G12D Mutant [NBP3-13647]](https://resources.bio-techne.com/images/products/Ras-Antibody-HL10-G12D-Mutant-Immunohistochemistry-Paraffin-NBP3-13647-img0003.jpg "Immunohistochemistry-Paraffin: Ras Antibody (HL10) - G12D Mutant [NBP3-13647]")
Immunohistochemistry-Paraffin: Ras Antibody (HL10) - G12D Mutant [NBP3-13647]
Immunohistochemistry-Paraffin: Ras Antibody (HL10) - G12D Mutant [NBP3-13647] - Ras (G12D Mutant) antibody [HL10] detects Ras (G12D Mutant) protein at by immunohistochemical analysis. Sample: Paraffin-embedded KPC mouse pancreatic tumor cells implanted in the pancreas of B6 mice (left) and normal mouse pancreas (right). Ras (G12D Mutant) stained by Ras (G12D Mutant) antibody [HL10] (NBP3-13647) diluted at 1:75. Antigen Retrieval: Citrate buffer, pH 6.0, 40 min![Western Blot: Ras Antibody (HL10)G12D Mutant [NBP3-13647]](https://resources.bio-techne.com/images/products/Ras-Antibody-HL10-G12D-Mutant-Western-Blot-NBP3-13647-img0002.jpg "Western Blot: Ras Antibody (HL10)G12D Mutant [NBP3-13647]")
Western Blot: Ras Antibody (HL10)G12D Mutant [NBP3-13647]
Western Blot: Ras Antibody (HL10) - G12D Mutant [NBP3-13647] - Various whole cell extracts were separated by SDS-PAGE, and the membrane was blotted with Ras (G12D Mutant) antibody [HL10] (NBP3-13647) diluted at 1:1000.![Immunohistochemistry-Paraffin: Ras Antibody (HL10) - G12D Mutant [NBP3-13647]](https://resources.bio-techne.com/images/products/Ras-Antibody-HL10-G12D-Mutant-Immunohistochemistry-Paraffin-NBP3-13647-img0001.jpg "Immunohistochemistry-Paraffin: Ras Antibody (HL10) - G12D Mutant [NBP3-13647]")
Applications for Ras Antibody (HL10) - G12D Mutant - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
1:25-1:100
Immunohistochemistry-Paraffin
1:25-1:100
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Ras
The Ras proteins (H-Ras, N-Ras, and two splice variants of K-Ras) define a closely related family of 21 kDa GTPases that cycle between GTP-bound active and GDP-bound inactive states at the cytoplasmic face of the plasma membrane. Receptor tyrosine kinases and G-protein coupled receptors activate Ras, and effectors include Raf and PI 3-kinase. Activating mutations of Ras, which impair GTPase activity and stabilize the active state, are found in nearly one-third of all human cancers.
Long Name
v-Ki-ras2 Kirsten Rat Sarcoma Viral Oncogene Jomolog
Alternate Names
c-K-ras, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, Ki-Ras, KRAS, KRAS1, KRAS2, Oncogene Ras, RASK2
Additional Ras Products
Product Documents for Ras Antibody (HL10) - G12D Mutant - Azide and BSA Free
Product Specific Notices for Ras Antibody (HL10) - G12D Mutant - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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