POU3F3 Antibody (CL15308) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44292

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL15308

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all POU3F3 Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of P20264, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for POU3F3 Antibody (CL15308) - Azide and BSA Free

POU3F3 Antibody (CL15308) - Azide and BSA Free Immunohistochemistry-Paraffin: POU3F3 Antibody [NBP3-44292]

Immunohistochemistry-Paraffin: POU3F3 Antibody [NBP3-44292]

Analysis in human kidney and skeletal muscle tissues using NBP3-44292 antibody. Corresponding POU3F3 RNA-seq data are presented for the same tissues.
POU3F3 Antibody (CL15308) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: POU3F3 Antibody [NBP3-44292]

Immunocytochemistry/ Immunofluorescence: POU3F3 Antibody [NBP3-44292]

Staining in SH-SY5Y cell line with Anti-POU3F3 monoclonal antibody) .
POU3F3 Antibody (CL15308) - Azide and BSA Free Immunohistochemistry-Paraffin: POU3F3 Antibody [NBP3-44292]

Immunohistochemistry-Paraffin: POU3F3 Antibody [NBP3-44292]

Staining of human cerebellum shows strong nuclear positivity in granular layer and molecular layer cells.
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Applications for POU3F3 Antibody (CL15308) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:1000-1:2500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: POU3F3

POU3F3, also known as POU Class 3 Homeobox 3, is a transcription factor that is primarily expressed in the brain and CNS. POU3F3 interacts with SOX4 and SOX11, and is implicated in the development of neurons. Current research surrounding POU3F3 has shown potential linkages with neuronitis and cerebritis. POU3F3 has also been shown to interact with POU3F4, CAV1, SOX10, and GADD34.

Alternate Names

Brain-1, Brain-specific homeobox/POU domain protein 1, Brn-1, BRN1brn-1, Oct-8, Octamer-binding protein 8, Octamer-binding transcription factor 8, OTF-8, OTF8oct-8, POU class 3 homeobox 3, POU domain class 3, transcription factor 3, POU domain, class 3, transcription factor 3

Gene Symbol

POU3F3

Additional POU3F3 Products

Product Documents for POU3F3 Antibody (CL15308) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for POU3F3 Antibody (CL15308) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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