POU3F3 Antibody (CL15308) [Unconjugated] - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43660
![POU3F3 Antibody (CL15308) [Unconjugated] Immunohistochemistry-Paraffin: POU3F3 Antibody (CL15308) [NBP3-43660]](https://resources.bio-techne.com/images/products/nbp3-43660_mouse-pou3f3-mab-cl15308-24120251545898.jpg "Immunohistochemistry-Paraffin: POU3F3 Antibody (CL15308) [NBP3-43660]")
![POU3F3 Antibody (CL15308) [Unconjugated] Immunohistochemistry-Paraffin: POU3F3 Antibody (CL15308) [NBP3-43660]](https://resources.bio-techne.com/images/products/nbp3-43660_mouse-pou3f3-mab-cl15308-24120251545893.jpg "Immunohistochemistry-Paraffin: POU3F3 Antibody (CL15308) [NBP3-43660]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human, Mouse
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # CL15308
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
This antibody was generated using a synthetic peptide of P20264, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for POU3F3 Antibody (CL15308) [Unconjugated] - BSA Free
Immunohistochemistry-Paraffin: POU3F3 Antibody (CL15308) [NBP3-43660]
Staining of human skeletal muscle shows no positivity in myocytes as expected.![POU3F3 Antibody (CL15308) [Unconjugated] Immunohistochemistry-Paraffin: POU3F3 Antibody (CL15308) [NBP3-43660]](https://resources.bio-techne.com/images/products/nbp3-43660_mouse-pou3f3-mab-cl15308-24120251545875.jpg "Immunohistochemistry-Paraffin: POU3F3 Antibody (CL15308) [NBP3-43660]")
Immunohistochemistry-Paraffin: POU3F3 Antibody (CL15308) [NBP3-43660]
Staining of human cerebellum shows strong nuclear positivity in granular layer and molecular layer cells.![POU3F3 Antibody (CL15308) [Unconjugated] Immunohistochemistry-Paraffin: POU3F3 Antibody (CL15308) [NBP3-43660]](https://resources.bio-techne.com/images/products/nbp3-43660_mouse-pou3f3-mab-cl15308-241202515454948.jpg "Immunohistochemistry-Paraffin: POU3F3 Antibody (CL15308) [NBP3-43660]")
Immunohistochemistry-Paraffin: POU3F3 Antibody (CL15308) [NBP3-43660]
Staining of human rectum shows no positivity in glandular cells as expected.![POU3F3 Antibody (CL15308) [Unconjugated] Western blot: POU3F3 Antibody (CL15308) [NBP3-43660]](https://resources.bio-techne.com/images/products/nbp3-43660_mouse-pou3f3-mab-cl15308-241202515454958.jpg "Western blot: POU3F3 Antibody (CL15308) [NBP3-43660]")
![POU3F3 Antibody (CL15308) [Unconjugated] Immunohistochemistry-Paraffin: POU3F3 Antibody (CL15308) [NBP3-43660]](https://resources.bio-techne.com/images/products/nbp3-43660_mouse-pou3f3-mab-cl15308-24120251545821.jpg "Immunohistochemistry-Paraffin: POU3F3 Antibody (CL15308) [NBP3-43660]")
![POU3F3 Antibody (CL15308) [Unconjugated] Immunohistochemistry-Paraffin: POU3F3 Antibody (CL15308) [NBP3-43660]](https://resources.bio-techne.com/images/products/nbp3-43660_mouse-pou3f3-mab-cl15308-241202515454993.jpg "Immunohistochemistry-Paraffin: POU3F3 Antibody (CL15308) [NBP3-43660]")
![POU3F3 Antibody (CL15308) [Unconjugated] Immunohistochemistry-Paraffin: POU3F3 Antibody (CL15308) [NBP3-43660]](https://resources.bio-techne.com/images/products/nbp3-43660_mouse-pou3f3-mab-cl15308-2412025154580.jpg "Immunohistochemistry-Paraffin: POU3F3 Antibody (CL15308) [NBP3-43660]")
![POU3F3 Antibody (CL15308) [Unconjugated] Immunocytochemistry/ Immunofluorescence : POU3F3 Antibody (CL15308) [NBP3-43660]](https://resources.bio-techne.com/images/products/nbp3-43660_mouse-pou3f3-mab-cl15308-24120251553258.jpg "Immunocytochemistry/ Immunofluorescence: POU3F3 Antibody (CL15308) [NBP3-43660]")
Applications for POU3F3 Antibody (CL15308) [Unconjugated] - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:1000-1:2500
Immunohistochemistry-Paraffin
1:1000-1:2500
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, pH 7.2, 40% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: POU3F3
Alternate Names
Brain-1, Brain-specific homeobox/POU domain protein 1, Brn-1, BRN1brn-1, Oct-8, Octamer-binding protein 8, Octamer-binding transcription factor 8, OTF-8, OTF8oct-8, POU class 3 homeobox 3, POU domain class 3, transcription factor 3, POU domain, class 3, transcription factor 3
Gene Symbol
POU3F3
Additional POU3F3 Products
Product Documents for POU3F3 Antibody (CL15308) [Unconjugated] - BSA Free
Product Specific Notices for POU3F3 Antibody (CL15308) [Unconjugated] - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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