Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Chicken

Cited:

Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgM Clone # O4

View all Oligodendrocyte Marker O4 Antibodies »

Product Specifications

Immunogen

Bovine brain corpus callosum white matter

Specificity

Detects human, mouse, rat, and chicken Oligodendrocyte Marker O4.

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Scientific Data Images for  Oligodendrocyte Marker O4 APC-conjugated Antibody

Detection of Oligodendrocyte Marker O4 antibody in Differentiated Rat Cortical Stem Cells antibody by Flow Cytometry.

Detection of Oligodendrocyte Marker O4 in Differentiated Rat Cortical Stem Cells by Flow Cytometry.

Differentiated Rat Cortical Stem Cells (Catalog # NSC001) were stained with Mouse Anti-Human/Mouse/ Rat/Chicken Oligodendrocyte Marker O4 APC-conjugated Monoclonal Antibody (Catalog # FAB1326A, filled histogram) or isotype control antibody (Catalog # IC015A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for  Oligodendrocyte Marker O4 APC-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Differentiated Rat Cortical Stem Cells (Catalog # NSC001)

Reviewed Applications

Read 2 reviews rated 4.5 using FAB1326A in the following applications:

Formulation, Preparation, and Storage

Purification

IgM-specific Affinity-purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Oligodendrocyte Marker O4

Oligodendrocytes are myelinating cells in the central nervous system (CNS) and form the myelin sheath of axons to support rapid nerve conduction. Oligodendrocyte Marker O4 is an antigen on the surface of oligodendrocyte progenitors (1, 2). It has been commonly used as the earliest recognized marker specific for the oligodendroglial lineage (3-8).

References

  1. Schachner, M. et al. (1981) Dev. Biol. 83:328.
  2. Bansal, R. et al. (1989) J. Neurosci. Res. 24:548.
  3. Bansal, R. and Pfeiffer, S.E. (1989) Proc. Natl. Acad. Sci. USA 86:6181.
  4. Gard, A. et al. (1995) Dev. Biol. 167:596.
  5. Reynolds, R. and Hardy, R. (1997) J. Neurosci. Res. 47:455.
  6. Ono, K. et al. (1997) J. Neurosci. Res. 48:212.
  7. Pang, Y. et al. (2000) J. Neurosci. Res. 62:510.
  8. Cai, Z. et al. (2001) Brain Res. 898:126.

Additional Oligodendrocyte Marker O4 Products

Product Documents for  Oligodendrocyte Marker O4 APC-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for  Oligodendrocyte Marker O4 APC-conjugated Antibody

For research use only

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