Mouse/Rat Serpin F1/PEDF Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1149
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse, Rat
Cited:
Mouse, Rat
Applications
Validated:
Western Blot, Simple Western
Cited:
Western Blot, ELISA Development
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse Serpin F1/PEDF
Asp43-Thr417
Accession # BAA31978
Asp43-Thr417
Accession # BAA31978
Specificity
Detects mouse Serpin F1/PEDF in direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross-reactivity with recombinant human Serpin F1 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse/Rat Serpin F1/PEDF Antibody
Detection of Mouse and Rat Serpin F1/PEDF by Western Blot.
Western blot shows lysates of C2C12 mouse myoblast cell line, NIH-3T3 mouse embryonic fibroblast cell line, 3T3-L1 mouse embryonic fibroblast adipose-like cell line, NRK rat normal kidney cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse/Rat Serpin F1/PEDF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1149) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for Serpin F1/PEDF at approximately 46-48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse Serpin F1/PEDF by Simple WesternTM.
Simple Western lane view shows lysates of NIH-3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Serpin F1/PEDF at approximately 55 kDa (as indicated) using 20 µg/mL of Goat Anti-Mouse/Rat Serpin F1/PEDF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1149) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Serpin F1/PEDF by Western Blot
Western blot verification of selected muscle proteins commonly regulated by UND in both the PL and SOL muscles. (A,E) Representative western blot images and densitometric analysis of (B,F) beta-oxidation enzyme ACADM, (C,G) oxidative phosphorylation enzyme CYTC, and (D,H) glycoprotein SERPINF1 levels in PL and SOL muscles from UND vs. CTRL rats (n = 6/group). Results are expressed as means ± SEM. Unpaired student’s t-test was performed to test the effect of undernutrition (*, P < 0.05; **, P < 0.01). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35563153), licensed under a CC-BY license. Not internally tested by R&D Systems.
Applications for Mouse/Rat Serpin F1/PEDF Antibody
Application
Recommended Usage
Simple Western
20 µg/mL
Sample: NIH‑3T3 mouse embryonic fibroblast cell line
Sample: NIH‑3T3 mouse embryonic fibroblast cell line
Western Blot
0.2 µg/mL
Sample: C2C12 mouse myoblast cell line, NIH‑3T3 mouse embryonic fibroblast cell line, 3T3‑L1 mouse embryonic fibroblast adipose-like cell line, NRK rat normal kidney cell line, and Rat‑2 rat embryonic fibroblast cell line
Sample: C2C12 mouse myoblast cell line, NIH‑3T3 mouse embryonic fibroblast cell line, 3T3‑L1 mouse embryonic fibroblast adipose-like cell line, NRK rat normal kidney cell line, and Rat‑2 rat embryonic fibroblast cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Serpin F1/PEDF
Long Name
Serpine F1/Pigment Epithelium-derived Factor
Alternate Names
EPC-1, PEDF
Gene Symbol
SERPINF1
UniProt
Additional Serpin F1/PEDF Products
Product Documents for Mouse/Rat Serpin F1/PEDF Antibody
Product Specific Notices for Mouse/Rat Serpin F1/PEDF Antibody
For research use only
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