Detects mouse PRAT4A in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse PRAT4B is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse PRAT4A Antibody

Detection of Mouse PRAT4A by Western Blot.

Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line and RAW 264.7 mouse monocyte/macrophage cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse PRAT4A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4429) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PRAT4A at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

PRAT4A in Mouse Brain.

PRAT4A was detected in immersion fixed paraffin-embedded sections of mouse brain (thalamus) using Goat Anti-Mouse PRAT4A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4429) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Mouse PRAT4A Antibody

Application

Recommended Usage

Immunohistochemistry

1-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of mouse brain (thalamus)

Western Blot

1 µg/mL
Sample: Neuro‑2A mouse neuroblastoma cell line and RAW 264.7 mouse monocyte/macrophage cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PRAT4A

PRAT4A (Protein associated with TLR4-A) is a ubiquitously expressed, 40 kDa protein that participates in Toll receptor expression. Mouse PRAT4A is 276 amino acids (aa) in length. It contains a putative signal sequence of 37 aa, followed by a 239 aa mature region. Although the presence of a signal sequence suggests it is secreted, its best known activity involves an intracellular association with TLR4. PRAT4A appears to facilitate the maturation and expression of TLR4 on the cell surface. Studies suggest this involves the C-terminal 60 amino acids. Mature mouse PRAT4A shares 90% and 96% aa identity with human and rat PRAT4A, respectively.

Long Name

Protein Associated with Toll-like Receptor 4A

Alternate Names

CAG4A, CNPY3, ERDA5, TNRC5

Entrez Gene IDs

10695 (Human); 72029 (Mouse); 316225 (Rat)

Gene Symbol

CNPY3

UniProt

Q9DAU1

Additional PRAT4A Products

Product Documents for Mouse PRAT4A Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse PRAT4A Antibody

For research use only

Citations
Reviews

Mouse PRAT4A Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4429

Key Product Details

Species Reactivity

Validated:

Cited:

Applications