Mouse Oncostatin M/OSM Alexa Fluor™ Plus 647-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF-495-NAAFP647
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot, Neutralization
Label
Alexa Fluor Plus 647 (Excitation = 658 nm, Emission = 675 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse OSM
Specificity
Detects mouse OSM in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human OSM is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications
Application
Recommended Usage
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Neutralization
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Oncostatin M/OSM
Oncostatin M (OSM) is a member of a cytokine subfamily that includes IL-6, IL-11, LIF, CNTF, and cardiotrophin-1. These cytokines have overlapping biological functions and shared receptor components. Mouse OSM was cloned and identified as an immediate early gene induced in various myeloid and lymphoid cell lines by a subset of cytokines including IL-2, IL-3, GM-CSF, and EPO. The mouse OSM cDNA encodes a 263 amino acid residue precursor protein that shows 48% identity with human OSM. Similar to human OSM, the C-terminal region of mouse OSM contains a highly charged region. Deletion of this C-terminal region appears to be essential for the formation of biologically active mOSM.
The biological activity of human OSM has been shown to be mediated either by the LIF/OSM receptor complex composed of gp130 and LIF R alpha or by a human OSM specific receptor composed of gp130 and OSM R alpha. It remains to be determined if the biological activities of mouse OSM can also be mediated by both receptor complexes in mouse cells.
References
- Yoshimura, A. et al. (1996) The EMBO Journal 15:1055.
- Ray, P. et al. (1996) Endocrinology 137:1151.
- Rose, T.M. and A.G. Bruce (1994) in Guidebook to Cytokines and Their Receptors, N.A. Nicola, editor, Oxford University Press, New York, p. 127.
Alternate Names
MGC20461, oncostatin M, oncostatin-M
Gene Symbol
OSM
UniProt
Additional Oncostatin M/OSM Products
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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