Mouse Neuregulin-3/NRG3 EGF-like Domain Antibody
R&D Systems, part of Bio-Techne | Catalog # AF753
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse NRG3
Glu284-Pro344
Accession # O35181
Glu284-Pro344
Accession # O35181
Specificity
Detects mouse NRG3 EGF-like domain in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse Neuregulin-3/NRG3 EGF-like Domain Antibody
Neuregulin‑3/NRG3 in Mouse Brain.
Neuregulin-3/NRG3 was detected in perfusion fixed frozen sections of mouse brain (hippocampus) using Goat Anti-Mouse Neuregulin-3/NRG3 EGF-like Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF753) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cell bodies and neuronal processes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Mouse Neuregulin-3/NRG3 EGF-like Domain Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse brain (hippocampus)
Sample: Perfusion fixed frozen sections of mouse brain (hippocampus)
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse Neuregulin-3/NRG3 EGF-like Domain
Sample: Recombinant Mouse Neuregulin-3/NRG3 EGF-like Domain
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Neuregulin-3/NRG3
The neuregulin (also known as heregulin) cytokine family is comprised of four genes that encode a large number of secreted or membrane-bound isoforms. All family members share an EGF-like domain that interacts with the ErbB family of tyrosine kinase receptors. The NRG1 gene is the most pleiotropic, encoding more than 14 soluble or transmembrane proteins. NRG1 isoforms can be classified into type I (neu differentiation factor, NDF; heregulin, HRG; acetylcholine receptor inducing activity, ARIA), type II (glial growth factor, GGF) and type III (sensory and motor neuron-derived factor, SMDF) isoforms. NRG proteins show distinct spatial and temporal expression patterns and play important roles during development of both the nervous system and the heart.
Alternate Names
Neuregulin3, NRG3
Gene Symbol
NRG3
UniProt
Additional Neuregulin-3/NRG3 Products
Product Documents for Mouse Neuregulin-3/NRG3 EGF-like Domain Antibody
Product Specific Notices for Mouse Neuregulin-3/NRG3 EGF-like Domain Antibody
For research use only
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