Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all Lymphotoxin beta R/TNFRSF3 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0 derived recombinant mouse Lymphotoxin beta Receptor
Ser28-Pro218
Accession # P50284

Specificity

Detects mouse Lymphotoxin betaR/TNFRSF3 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse Lymphotoxin  betaR/TNFRSF3 Antibody

Detection of Mouse Lymphotoxin betaR/TNFRSF3 antibody by Western Blot.

Detection of Mouse Lymphotoxin betaR/TNFRSF3 by Western Blot.

Western blot shows lysates of C2C12 mouse myoblast cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Mouse Lymphotoxin betaR/TNFRSF3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF10081) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Lymphotoxin betaR/TNFRSF3 at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Lymphotoxin betaR/TNFRSF3 antibody in Mouse Liver by Immunohistochemistry (IHC-Fr).

Lymphotoxin betaR/TNFRSF3 in Mouse Liver.

Lymphotoxin betaR/TNFRSF3 was detected in perfusion fixed frozen sections of mouse liver using Sheep Anti-Mouse Lymphotoxin betaR/TNFRSF3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF10081) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces in hepatocytes. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Applications for Mouse Lymphotoxin  betaR/TNFRSF3 Antibody

Application
Recommended Usage

Immunohistochemistry

1-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse liver

Western Blot

2 µg/mL
Sample: C2C12 mouse myoblast cell line, Hepa 1‑6 mouse hepatoma cell line, and Mouse stomach tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lymphotoxin beta R/TNFRSF3

Lymphotoxin beta receptor (LT betaR), also known as TNF RIII and TNF R-related protein (TNF Rrp) is a member of the TNF receptor superfamily, designated TNFRSF3. Human LT betaR cDNA encodes a 435 amino acid (aa) residue type I membrane protein with a putative 30 aa residue signal peptide, a 193 aa residue extracellular domain and a 171 aa residue cytoplasmic domain. The extracellular domain of LT betaR contains four cysteine-rich motifs characteristic of the TNF receptor superfamily. The cytoplasmic region of LT betaR shares little sequence similarity with other TNF receptor family members, suggesting that different signaling mechanisms may be used. LT betaR is expressed in a variety of tissues including visceral and lymphoid tissues. LT betaR is also expressed by cell lines of monocytic, epithelial, and fibroblastic origins but not by T and B lymphocytes. Human and mouse LT betaR share 76% aa sequence homology. The TNF family ligands that have been shown to bind and activate LT betaR include LIGHT (also a ligand for HVEM) and the heterotrimeric lymphotoxin LT alpha1/ beta2 or LT alpha2/ beta1. Depending on the cell type, activation of LT betaR has been shown to induce NF kappaB activation, chemokine production, growth arrest, and apoptosis. In vivo, LT betaR has been shown to play a critical role in controlling cellular immune functions and lymphoid organogenesis.

References

  1. Zhai, Y. et al. (1998) J. Clin. Invest. 102:1142.
  2. Rennert, P.D. et al. (1998) Immunity 9:71..
  3. Degli-Esposti, M.A. et al. (1997) J. Immunol 158:1756. .
  4. Mackay, F. et al. (1996) J. Biol. Chem. 271:8618. .
  5. Crowe, P.D. et al. (1994) Science 264:707.

Long Name

Lymphotoxin beta Receptor

Alternate Names

LTBR, LymphotoxinbR, TNF RIII, TNF Rrp, TNFRSF3

Entrez Gene IDs

4055 (Human); 17000 (Mouse); 297604 (Rat); 102135920 (Cynomolgus Monkey); 712550 (Rhesus Macaque)

Gene Symbol

LTBR

UniProt

P50284

Additional Lymphotoxin beta R/TNFRSF3 Products

Product Documents for Mouse Lymphotoxin  betaR/TNFRSF3 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Mouse Lymphotoxin  betaR/TNFRSF3 Antibody

For research use only

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