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Mouse IL-4 MAb (Clone 30340)

Catalog # MAB404 | R&D Systems, Inc. a Bio-Techne Brand
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MAB404-100
MAB404-500
MAB404-SP

Key Product Details

Species Reactivity

Mouse

Applications

ELISA Capture (Matched Antibody Pair), Neutralization, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 30340

Product Summary for Mouse IL-4 MAb (Clone 30340)

Immunogen

E. coli-derived recombinant mouse IL-4
His23-Ser140
Accession # P07750

Specificity

Detects mouse IL-4 in ELISAs and Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Mouse IL-4 MAb (Clone 30340)

Cell Proliferation Induced by IL‑4 and Neutralization by Mouse IL‑4 Antibody.

Cell Proliferation Induced by IL‑4 and Neutralization by Mouse IL‑4 Antibody.

Recombinant Mouse IL-4 (Catalog # 404-ML) stimulates proliferation in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat Anti-Mouse IL-4 (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-4 Monoclonal Antibody (Catalog # MAB404). The ND50 is typically 0.1-0.6 µg/mL.

Applications for Mouse IL-4 MAb (Clone 30340)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse IL‑4 (Catalog # 404-ML)
under non-reducing conditions only

Neutralization

Measured by its ability to neutralize IL‑4-induced proliferation in the HT‑2 mouse T cell line. The Neutralization Dose (ND50) is typically 0.1-0.6 µg/mL in the presence of 7.5 ng/mL Recombinant Mouse IL‑4.

Mouse IL-4 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse IL‑4 Biotinylated Antibody (Catalog # BAF404)
  • Standard: Recombinant Mouse IL-4 Protein (Catalog # 404-ML)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB404 in the following applications:

Formulation, Preparation and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-4

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13-18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑4). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha-helix structure (5). Mouse IL-4 is synthesized with a 24 amino acid (aa) signal sequence. Mature mouse IL-4 shares 39%, 39%, and 59% aa sequence identity with bovine, human, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (6-8). IL-4 exerts its effects through two receptor complexes (9, 10). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG1 and IgE in mouse B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (11‑14). IL-4 plays a dominant role in the development of allergic inflammation and asthma (13, 15).

References

  1. Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.
  2. Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
  3. Lee, F. et al. (1986) Proc. Natl. Acad. Sci. USA 83:2061.
  4. Noma, Y. et al. (1986) Nature 319:640.
  5. Redfield, C. et al. (1991) Biochemistry 30:11029.
  6. Ramirez, F. et al. (1988) J. Immunol. Meth. 221:141.
  7. Leitenberg, D. and T.L. Feldbush (1988) Cell. Immunol. 111:451.
  8. Mosman, T.R. et al. (1987) J. Immunol. 138:1813.
  9. Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.
  10. Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.
  11. Paludan, S.R. (1998) Scand. J. Immunol. 48:459.
  12. Corthay, A. (2006) Scand. J. Immunol. 64:93.
  13. Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.
  14. Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.
  15. Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.

Long Name

Interleukin 4

Alternate Names

BCDF, BCGF-1, BCGF1, BSF-1, BSF1, IL4, IL4E12

Entrez Gene IDs

280824 (Bovine); 403785 (Canine); 3565 (Human); 16189 (Mouse); 397225 (Porcine); 574281 (Primate); 100302454 (Rabbit); 287287 (Rat)

Gene Symbol

IL4

UniProt

Product Documents for Mouse IL-4 MAb (Clone 30340)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse IL-4 MAb (Clone 30340)

For research use only

Citations for Mouse IL-4 MAb (Clone 30340) (26)

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Customer Reviews for Mouse IL-4 MAb (Clone 30340) (1)

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  • Mouse IL-4 Antibody
    Name: Michelle Chen
    Application: Functional Assay
    Sample Tested: Peripheral blood neutrophils
    Species: Human
    Verified Customer | Posted 05/10/2017

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