Mouse IL-4 MAb (Clone 30340)
Catalog # MAB404 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Summary for Mouse IL-4 MAb (Clone 30340)
Immunogen
His23-Ser140
Accession # P07750
Specificity
Clonality
Host
Isotype
Scientific Data Images for Mouse IL-4 MAb (Clone 30340)
Cell Proliferation Induced by IL‑4 and Neutralization by Mouse IL‑4 Antibody.
Recombinant Mouse IL-4 (Catalog # 404-ML) stimulates proliferation in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat Anti-Mouse IL-4 (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-4 Monoclonal Antibody (Catalog # MAB404). The ND50 is typically 0.1-0.6 µg/mL.Applications for Mouse IL-4 MAb (Clone 30340)
Western Blot
Sample: Recombinant Mouse IL‑4 (Catalog # 404-ML)
under non-reducing conditions only
Neutralization
Mouse IL-4 Sandwich Immunoassay
Reviewed Applications
Read 1 review rated 5 using MAB404 in the following applications:
Published Applications
Read 26 publications using MAB404 in the following applications:
Formulation, Preparation and Storage
Purification
Endotoxin Level
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-4
Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13-18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑4). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha-helix structure (5). Mouse IL-4 is synthesized with a 24 amino acid (aa) signal sequence. Mature mouse IL-4 shares 39%, 39%, and 59% aa sequence identity with bovine, human, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (6-8). IL-4 exerts its effects through two receptor complexes (9, 10). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG1 and IgE in mouse B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (11‑14). IL-4 plays a dominant role in the development of allergic inflammation and asthma (13, 15).
References
- Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.
- Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
- Lee, F. et al. (1986) Proc. Natl. Acad. Sci. USA 83:2061.
- Noma, Y. et al. (1986) Nature 319:640.
- Redfield, C. et al. (1991) Biochemistry 30:11029.
- Ramirez, F. et al. (1988) J. Immunol. Meth. 221:141.
- Leitenberg, D. and T.L. Feldbush (1988) Cell. Immunol. 111:451.
- Mosman, T.R. et al. (1987) J. Immunol. 138:1813.
- Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.
- Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.
- Paludan, S.R. (1998) Scand. J. Immunol. 48:459.
- Corthay, A. (2006) Scand. J. Immunol. 64:93.
- Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.
- Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.
- Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
UniProt
Product Documents for Mouse IL-4 MAb (Clone 30340)
Product Specific Notices for Mouse IL-4 MAb (Clone 30340)
For research use only
Citations for Mouse IL-4 MAb (Clone 30340) (26)
Citations are publications that use Bio-Techne products. Selected citations for Mouse IL-4 MAb (Clone 30340) include:
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Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western BlotD Chen et al. (2022-12-01), Interleukin-4 promotes microglial polarization toward a neuroprotective phenotype after retinal ischemia/reperfusion injury Oncogene, 2022-117(12):2755-2760.
PMID: 35662225 -
Species: Mouse
Sample Types: Whole Cells
Applications: NeutralizationJS Park et al. (2022-11-17), EC-18 prevents autoimmune arthritis by suppressing inflammatory cytokines and osteoclastogenesis Arthritis Research & Therapy, 2022-124(1):254.
PMID: 36397156 -
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA CaptureK Kasahara et al. (2022-07-19), Depletion of Foxp3+ regulatory T cells augments CD4+ T cell immune responses in atherosclerosis-prone hypercholesterolemic mice Heliyon, 2022-08(7):e09981.
PMID: 35898604 -
Species: Mouse
Sample Types: Whole Cells
Applications: NeutralizationJ Zhou et al. (2021-05-12), The YAP/HIF-1&alpha/miR-182/EGR2 axis is implicated in asthma severity through the control of Th17 cell differentiation Cell & bioscience, 2021-011(1):84.
PMID: 33980319 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayJQ Yang et al. (2019-07-01), Ablation of RhoA impairs Th17 cell differentiation and alleviates house dust mite-triggered allergic airway inflammation J. Leukoc. Biol., 2019-00(0).
PMID: 31260596 -
Species: Mouse
Sample Types: Whole Cells
Applications: Functional AssayY Nam et al. (2018-06-07), Intraperitoneal infusion of mesenchymal stem cell attenuates severity of collagen antibody induced arthritis PLoS ONE, 2018-013(6):e0198740.
PMID: 29879214 -
Species: Mouse
Sample Types: Whole Cells
Applications: ELISA CaptureM Ramírez et al. (2018-03-07), Characterization of the immune response elicited by the vaccinia virus L3 protein delivered as naked DNA Vaccine, 2018-036(15):2049-2055.
PMID: 29525282 -
Species: Mouse
Sample Types: Whole Cells
Applications: NeutralizationY Hu et al. (2017-02-07), Armc5 deletion causes developmental defects and compromises T-cell immune responses Nat Commun, 2017-08(0):13834.
PMID: 28169274 -
Species: Mouse
Sample Types: Whole Cells
Applications: NeutralizationNata T et al. (2015-07-16), Targeting the binding interface on a shared receptor subunit of a cytokine family enables the inhibition of multiple member cytokines with selectable target spectrum. J Biol Chem, 2015-0290(37):22338-51.
PMID: 26183780 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayLee H et al. (2015-03-13), Mesenchymal stem/stromal cells protect against autoimmunity via CCL2-dependent recruitment of myeloid-derived suppressor cells. J Immunol, 2015-0194(8):3634-45.
PMID: 25769927 -
Species: Mouse
Sample Types: Whole Cells
Applications: NeutralizationSolt L et al. (2015-01-05), ROR inverse agonist suppresses insulitis and prevents hyperglycemia in a mouse model of type 1 diabetes. Endocrinology, 2015-0156(3):869-81.
PMID: 25560829 -
Species: Mouse
Sample Types: Whole Cells
Applications: NeutralizationReba S et al. (2014-03-14), TLR2 engagement on CD4(+) T cells enhances effector functions and protective responses to Mycobacterium tuberculosis. Eur J Immunol, 2014-044(5):1410-21.
PMID: 24497180 -
Species: Mouse
Sample Types: Whole Cells
Applications: NeutralizationTerra R et al. (2013-12-31), To investigate the necessity of STRA6 upregulation in T cells during T cell immune responses. PLoS ONE, 2013-18(12):e82808.
PMID: 24391722 -
Species: Mouse
Sample Types: Whole Cells
Applications: NeutralizationGomez M et al. (2013-06-12), Basophils control T-cell responses and limit disease activity in experimental murine colitis. Mucosal Immunol, 2013-07(1):188-99.
PMID: 23757302 -
Species: Mouse
Sample Types: Pleural Lavage Fluid
Applications: ELISA DevelopmentCosta MF et al. (2012-05-01), CCL25 induces alpha 4 beta 7 integrin-dependent migration of IL-17+ gamma/delta T lymphocytes during an allergic reaction. Eur. J. Immunol., 2012-042(5):1250-60.
PMID: 22539297 -
Species: Mouse
Sample Types: Plasma
Applications: Luminex DevelopmentZhang M et al. (2012-01-25), Response patterns of cytokines/chemokines in two murine strains after irradiation. Cytokine, 2012-058(2):169-77.
PMID: 22277799 -
Species: Mouse
Sample Types: Whole Cells
Applications: NeutralizationJin W et al. (2011-12-19), The effect of conditional EFNB1 deletion in the T cell compartment on T cell development and function. BMC Immunol., 2011-112(0):68.
PMID: 22182253 -
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA DevelopmentWieland CW et al. (2009-03-13), ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis. Microbes Infect., 2009-011(4):524-30.
PMID: 19286469 -
Species: Mouse
Sample Types: Whole Cells
Applications: NeutralizationMcKinley L et al. (2008-09-15), TH17 cells mediate steroid-resistant airway inflammation and airway hyperresponsiveness in mice. J. Immunol., 2008-0181(6):4089-97.
PMID: 18768865 -
Species: Mouse
Sample Types: In Vivo
Applications: NeutralizationMabalirajan U et al. (2008-09-01), Mitochondrial structural changes and dysfunction are associated with experimental allergic asthma. J. Immunol., 2008-0181(5):3540-8.
PMID: 18714027 -
Species: Mouse
Sample Types: In Vivo
Applications: NeutralizationIto H et al. (2008-05-16), Role of Valpha14+ NKT cells in the development of Hepatitis B virus-specific CTL: activation of Valpha14+ NKT cells promotes the breakage of CTL tolerance. Int. Immunol., 2008-020(7):869-79.
PMID: 18487227 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA DevelopmentWieland CW et al. (2008-04-03), CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis. Immunology, 2008-0125(2):272-9.
PMID: 18393969 -
Species: Mouse
Sample Types: Whole Cells
Applications: NeutralizationChiang YM et al. (2006-10-19), Cytopiloyne, a novel polyacetylenic glucoside from Bidens pilosa, functions as a T helper cell modulator. J Ethnopharmacol, 2006-1110(3):532-8.
PMID: 17101254 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA DevelopmentWen H et al. (2006-06-01), Severe sepsis exacerbates cell-mediated immunity in the lung due to an altered dendritic cell cytokine profile. Am. J. Pathol., 2006-0168(6):1940-50.
PMID: 16723709 -
Species: Mouse
Sample Types: Whole Cells
Applications: ELISpot DevelopmentBednarski JJ et al. (2003-03-01), Attenuation of autoimmune disease in Fas-deficient mice by treatment with a cytotoxic benzodiazepine. Arthritis Rheum., 2003-048(3):757-66.
PMID: 12632430 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA DevelopmentLukacs NW et al. (2002-04-01), AMD3100, a CxCR4 antagonist, attenuates allergic lung inflammation and airway hyperreactivity. Am. J. Pathol., 2002-0160(4):1353-60.
PMID: 11943720
There are no citations that match your criteria.
Customer Reviews for Mouse IL-4 MAb (Clone 30340) (1)
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