Recombinant Mouse IL-4 Protein

Catalog # 404-ML | R&D Systems, Inc. a Bio-Techne Brand
Recombinant Mouse IL-4 Protein Bioactivity
(2)
Recombinant Mouse IL-4 Protein SDS-PAGE
Catalog #
Availability
Size / Price
Qty
With Carrier
404-ML-100
404-ML-050
404-ML-025
404-ML-010
Catalog #
Availability
Size / Price
Qty
Carrier Free
404-ML-025/CF
404-ML-100/CF
404-ML-01M/CF
404-ML-050/CF
404-ML-010/CF
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Setting the standard in quality research reagents

Key Product Details

Source: E. coli
Accession #: P07750
Applications: Bioactivity
  • Formulation, Preparation & Storage

    • Formulation, Preparation and Storage

    Carrier Free
    What does CF mean?

    CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

    404-ML
    Carrier
    Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
    Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
    Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
    Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
    404-ML/CF
    Carrier Free
    Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
    Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
    Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
    Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
  • Citations
  • Reviews
  • FAQs

    • Citations for Recombinant Mouse IL-4 Protein (421)

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    Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse IL-4 Protein include:

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    Customer Reviews for Recombinant Mouse IL-4 Protein (17)

    4.9 out of 5
    17 customer ratings
    5 stars
    88%
    4 stars
    12%
    3 stars
    0%
    2 stars
    0%
    1 star
    0%

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    Showing  1 - 5 of 17 reviews Showing All
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    • Recombinant Mouse IL-4 Protein
      Name: Anonymous
      Application: Cell migration/motility
      Verified Customer | Posted 07/25/2023
      Recombinant Mouse IL-4 Protein Cell migration/motility 404-ML
    • Recombinant Mouse IL-4 Protein
      Name: Katie Myers
      Application: Immunoassay Standard
      Verified Customer | Posted 11/14/2022
      Used as a standard in our mouse ELISA.
      Recombinant Mouse IL-4 Protein Immunoassay Standard 404-ML
    • Recombinant Mouse IL-4 Protein
      Name: Anonymous
      Application: Enzymatic activity in vitro
      Verified Customer | Posted 09/29/2021
    • Recombinant Mouse IL-4 Protein
      Name: Marina Theodorou
      Application: CellProlif
      Verified Customer | Posted 05/20/2021
    • Recombinant Mouse IL-4 Protein
      Name: Anonymous
      Application: In vitro bioactivity in cell culture
      Verified Customer | Posted 04/13/2021
    • Recombinant Mouse IL-4 Protein
      Name: Anonymous
      Application: In vitro bioactivity in cell culture
      Verified Customer | Posted 01/27/2021
    • Recombinant Mouse IL-4 Protein
      Name: Anonymous
      Application: CellProlif
      Verified Customer | Posted 01/05/2021
    • Recombinant Mouse IL-4 Protein
      Name: Anonymous
      Application: CellProlif
      Verified Customer | Posted 01/05/2021
    • Recombinant Mouse IL-4 Protein
      Name: Gagandeep Kaur
      Application: CellProlif
      Verified Customer | Posted 11/09/2020
    • Recombinant Mouse IL-4 Protein
      Name: Yu Dang
      Application: In vitro bioactivity in cell culture
      Reason for Rating: IL-4 was used to differentiate THP-1 into M2 phenotype macrophages. We used immunofluorescence staining to show that after treatment the cells are CD206 positive, which means the treatment is successful in the phenotype change of THP-1 into M2 macrophage.
      Verified Customer | Posted 10/23/2020
      concentration :5ng/mlTime: 24 h
      Recombinant Mouse IL-4 Protein In vitro bioactivity in cell culture 404-ML
    • Recombinant Mouse IL-4 Protein
      Name: Supriya Sen
      Application: Immune-cell polarization.
      Verified Customer | Posted 10/06/2020
      Recombinant Mouse IL-4 Protein Other 404-ML
    • Recombinant Mouse IL-4 Protein
      Name: Yuqing Feng
      Application: In vitro bioactivity in cell culture
      Verified Customer | Posted 10/16/2019
    • Recombinant Mouse IL-4 Protein
      Name: Anonymous
      Application: CellProlif
      Verified Customer | Posted 07/13/2018
    • Recombinant Mouse IL-4 Protein
      Name: Danlee Enzler
      Application: In vitro bioactivity in cell culture
      Verified Customer | Posted 04/02/2018
    • Recombinant Mouse IL-4 Protein
      Name: Anonymous
      Application: Binding assay/Protein-protein interaction
      Reason for Rating: MAB404 binds rhuIL-13Ra2
      Verified Customer | Posted 02/07/2018
      Recombinant Mouse IL-4 Protein Binding assay/Protein-protein interaction 404-ML
    • Recombinant Mouse IL-4 Protein
      Name: Andrew Lechner
      Application: In vitro bioactivity in cell culture
      Verified Customer | Posted 07/21/2016
      We used recombinant mouse IL-4 to polarize bonemarrow-derived macrophages to an "M2" phenotype in vitro. We first generated macrophages from whole bone marrow using MCSF-rich media for 7 days. Then we added IL-4 at 20 ng/ml for 48 hours. Using IL-4R wild-type or knock-out bone marrow, we demonstrated using flow cytometry for an M2 reporter (Arginase1-YFP) that the IL-4 acts through the IL-4 receptor to polarize bone marrow derived macrophages towards the M2 phenotype.
      Recombinant Mouse IL-4 Protein In vitro bioactivity in cell culture 404-ML
    • Recombinant Mouse IL-4 Protein
      Name: Tima Thomas
      Application: In vitro bioactivity in cell culture
      Verified Customer | Posted 01/21/2016
      Recombinant Mouse IL-4 Protein In vitro bioactivity in cell culture 404-ML

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    Showing  1 - 5 of 17 reviews Showing All

    FAQs for Recombinant Mouse IL-4 Protein

    • Has recombinant mouse IL-4, Catalog # 404-ML, been tested for activity on human cells?

      We have not evaluated the bioactivity of Recombinant Mouse IL-4 Protein (Catalog # 404-ML) with human cells. However, we recommend reviewing our list of publications under the Citations tab on the product web page to find reported use of this product with human cells. We do offer a Recombinant Human IL-4 Protein (Catalog # 204-IL), which is suitable for use with human cells.

    • What concentration of recombinant mouse IL-4, Catalog # 404-ML, should I use for my cell culture experiment?

      We evaluate the bioactivity of Recombinant Mouse IL-4 Protein (Catalog # 404-ML) in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 0.5-1.5 ng/mL.  This ED50 range can provide a starting point for your experiment, but we would recommend end-users perform a dose-response to determine the best concentration for their assay system.  We also recommend reviewing our list of publications under the Citations tab on the product-specific web page to find reported use of our products in similar experimental layouts.  

    Product Documents for Recombinant Mouse IL-4 Protein

    Certificate of Analysis

    To download a Certificate of Analysis, please enter a lot number in the search box below.

    Note: Certificate of Analysis not available for kit components.

    Download SDS 404-ML (with carrier)
    Download SDS 404-ML/CF (carrier free)