Mouse IL-18/IL-1F4 Biotinylated Antibody

Name: Mouse IL-18/IL-1F4 Biotinylated Antibody
Brand: R&D Systems
SKU: D048-6

R&D Systems, Inc. a Bio-Techne Brand

Catalog # D048-6

Citations (4)

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Species Reactivity:	Mouse
Immunogen:	Mouse IL-18/IL-1F4
Applications:	ELISA Detection
Label:	Biotin
Source:	Monoclonal Rat IgG₁ Clone # 93-10C
Specificity:	This antibody reacts with mouse IL-18 on ELISA.

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Applications for Mouse IL-18/IL-1F4 Biotinylated Antibody

Mouse IL-18/IL-1F4 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.5-2.0 µg/mL

Use in combination with:

Capture Reagent: Mouse IL‑18/IL‑1F4 Antibody (Catalog # D047-3)

Standard: Recombinant Mouse IL-18/IL-1F4 Protein (Catalog # B002-5)

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Formulation	In 50 μL volume of PBS containing 1% BSA, 10% glycerol and 0.09% sodium azide, pH 7.2.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	This antibody solution is stable for one year from the date of purchase when stored at 4°C.

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Background: IL-18/IL-1F4

Interleukin 18 (IL-18) is a 18 kDa cytokine which identified as a costimulatory factor for production of interferon-gamma (IFN-gamma ) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24 kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN-gamma is produced by activated T or NK cells and plays critical roles in the defense against microbiral pathogens. IFN-gamma activates macrophages, enhances NK activity and B cell maturation, proliferation and Ig secretion, induces MHC class I and II antigens, and inhibits osteoclast activation. IL-18 acts on T helper type-1 (Th1) T cells and in combination with IL-12 strongly induces them to produce IFN-gamma. Pleiotropic effects of IL-18 has also been reported, such as, enhancement production of IFN-gamma and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF and IFN-gamma in T cells, enhancement of Fas ligand expression by Th1 cells.

Item	Value
References	McCurnin, D., et al., Pediatrics 121, 945-956 (2008) Haveman, L. M., et al., Int. Immunol. 18, 1521-1529 (2006) Shigehara, K., et al., J. Immunol. 166, 642-649 (2001) Dao, T., et al. Cell Immunol. 173, 230-235 (1996) Micallef, M., et al. Eur. J. Immunol. 26, 1647-1651 (1996) Ushio, S., et al. J. Immunol. 156, 4274-4279 (1996) Okamura, H., et al. Nature 378, 88-91 (1995)
Long Name	Interleukin 18
Entrez Gene IDs	3606 (Human); 16173 (Mouse); 29197 (Rat); 397057 (Porcine); 574151 (Primate)
Alternate Names	IFN-gamma-inducing factor, IGIF, IGIFIL-1 gamma, IL-18, IL-18MGC12320, IL-1F4, IL-1g, IL18, IL1F4iboctadekin, Iboctadekin, Interferon gamma-inducing factor, Interleukin-1 gamma, interleukin 18 (interferon-gamma-inducing factor), interleukin-18

Citations for Mouse IL-18/IL-1F4 Biotinylated Antibody (4)

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Citations are publications that use Bio-Techne products. Selected citations for Mouse IL-18/IL-1F4 Biotinylated Antibody include:

Species: Mouse
Sample Types: Peritoneal Lavage Fluid

H Ahn et al. (2020), Riboflavin, vitamin B2, attenuates NLRP3, NLRC4, AIM2, and non-canonical inflammasomes by the inhibition of caspase-1 activity Sci Rep, 10(1):19091.
PMID: 33154451
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA Development (Detection)

GM Castillo et al. (2017), Protected graft copolymer-formulated fibroblast growth factors mitigate the lethality of partial body irradiation injury PLoS ONE, 12(2):e0171703.
PMID: 28207794
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development

Hudson CA et al. (2008), Induction of IL-33 expression and activity in central nervous system glia. J. Leukoc. Biol., 84(3):631-43.
PMID: 18552204
Species: Mouse
Sample Types: Plasma
Applications: ELISA Development

Osuchowski MF et al. (2005), Sequential ELISA to profile multiple cytokines from small volumes. J. Immunol. Methods, 302(1):172-81.
PMID: 16023134

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Mouse IL-18/IL-1F4 Biotinylated Antibody

R&D Systems, Inc. a Bio-Techne Brand

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