Mouse CD3⁺splenocytes were stained with Rat Anti-Mouse IL-1 RI PE-conjugated Monoclonal Antibody (Catalog # FAB7712P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of IL-1 RI in RAW264 cells by Flow Cytometry.

RAW264 cells were stained with Rat Anti-Mouse IL-1 RI PE-conjugated Monoclonal Antibody (Catalog # FAB7712P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse IL-1 RI PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Mouse CD3⁺ splenocytes and RAW264 cells

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-1 RI

The type I IL-1 receptor (IL-1 RI, designated IL-1 R1 and CD121a) is one of at least nine members of the IL-1 R family within the Toll/IL-1 R (TIR) superfamily. IL‑1 RI is an ~80 kDa type I transmembrane (TM) protein that binds the pleiotropic cytokines IL-1 alpha and IL-1 beta, plus the IL-1 receptor antagonist (IL-1 Ra). Signal transduction requires complex formation with the IL-1 R accessory protein (IL-1 R AcP/IL-1 R3), another type I TM protein. This complex recruits the adaptor protein MyD88 to initiate signaling in the NF kappaB pathway. Mouse IL-1 RI cDNA encodes a 576 amino acid (aa) protein that contains a 19 aa signal sequence, a 319 aa extracellular domain (ECD) with three C2-type Ig-like domains, a 21 aa TM domain and a 217 aa cytoplasmic region with a TIR domain. Mouse IL-1 RI shares 64%, 83%, 60%, 61% and 55% aa identity with human, rat, canine, equine and bovine IL-1 RI, respectively. The role of IL-1 in inflammation is under several levels of control, including expression and activation of IL-1 alpha and IL-1 beta, expression of IL-1 RI and its accessory and adaptor proteins, and negative regulators such as the IL‑receptor family member, IL-1 RII/IL-1 R2. IL-1 RI is expressed predominantly by T cells, fibroblasts, and endothelial cells and mediates acute phase inflammatory responses including fever.

Long Name

Interleukin 1 Receptor I

Alternate Names

CD121a, IL-1RI, IL1R1, IL1RI

Entrez Gene IDs

3554 (Human); 16177 (Mouse)

Gene Symbol

IL1R1

UniProt

P13504

Additional IL-1 RI Products

Product Documents for Mouse IL-1 RI PE-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse IL-1 RI PE-conjugated Antibody

For research use only

Citations
Reviews

Mouse IL-1 RI PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB7712P

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all IL-1 RI Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse IL-1 RI PE-conjugated Antibody

Detection of IL‑1 RI in Mouse CD3+Splenocytes by Flow Cytometry.

Detection of IL-1 RI in RAW264 cells by Flow Cytometry.

Applications for Mouse IL-1 RI PE-conjugated Antibody

Flow Cytometry

Spectra Viewer

Plan Your Experiments

Formulation, Preparation, and Storage

Purification

Formulation

Shipping

Stability & Storage

Background: IL-1 RI

Long Name

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional IL-1 RI Products

Product Documents for Mouse IL-1 RI PE-conjugated Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for Mouse IL-1 RI PE-conjugated Antibody

Detection of IL‑1 RI in Mouse CD3⁺Splenocytes by Flow Cytometry.