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Rat IgG2B PE-conjugated Antibody
Catalog # IC013P | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Rat
Applications
Control
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Rat IgG2B Clone # 141945
Product Summary for Rat IgG2B PE-conjugated Antibody
Immunogen
KLH
Specificity
Selected for use as a rat IgG2B isotype control for flow cytometry applications.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Rat IgG2B PE-conjugated Antibody
Detection of Rat IgG Control by Flow Cytometry
Human peripheral blood mononuclear cells (PBMCs) were stained with Rat Anti-Human/Mouse CD44 PE-conjugated Monoclonal Antibody (Catalog # FAB6127P, filled histogram) or Rat IgG PE-conjugated Isotype Control Antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications for Rat IgG2B PE-conjugated Antibody
Application
Recommended Usage
Control
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Published Applications
Read 5 publications using IC013P in the following applications:
Formulation, Preparation and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG2B
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Long Name
Immunoglobulin G2B Fc
Alternate Names
IgG2b
Product Documents for Rat IgG2B PE-conjugated Antibody
Product Specific Notices for Rat IgG2B PE-conjugated Antibody
For research use only
Citations for Rat IgG2B PE-conjugated Antibody (5)
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Citations are publications that use Bio-Techne products. Selected citations for Rat IgG2B PE-conjugated Antibody include:
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Species: Mouse
Sample Types: Whole Cells
Applications: Flow CytometryM Felsenstei et al. (2020-07-13), CCR2 of Tumor Microenvironmental Cells Is a Relevant Modulator of Glioma Biology Cancers (Basel), 2020-012(7).
PMID: 32668709 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryS Sriram et al. (2018-12-18), Longitudinal changes in the expression of IL-33 and IL-33 regulated genes in relapsing remitting MS PLoS ONE, 2018-113(12):e0208755.
PMID: 30562364 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryZaidoon Al-Jaderi (2016-10-19), Glatiramer Acetate, Dimethyl Fumarate, and Monomethyl Fumarate Upregulate the Expression of CCR10 on the Surface of Natural Killer Cells and Enhance Their Chemotaxis and Cytotoxicity Front Immunol, 2016-17(0):437.
PMID: 27807435 -
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry ControlKumase F et al. (2016-01-22), AMPK-Activated Protein Kinase Suppresses Ccr2 Expression by Inhibiting the NF-kappaB Pathway in RAW264.7 Macrophages. PLoS ONE, 2016-011(1):e0147279.
PMID: 26799633 -
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry ControlZheng et al. (2013-11-18), Chemokine receptor CX3CR1 contributes to macrophage survival in tumor metastasis. Mol Cancer, 2013-112(1):141.
PMID: 24245985
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