Human/Mouse CD44 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB6127P

Recombinant Monoclonal Antibody.
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FAB6127P-025
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Conjugate
Catalog #
Unconjugated
MAB6127
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Alexa Fluor 350
FAB6127U
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Alexa Fluor 405
FAB6127V
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Alexa Fluor 488
FAB6127G
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Alexa Fluor 532
FAB6127X
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Alexa Fluor 594
FAB6127T
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Alexa Fluor 647
FAB6127R
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Alexa Fluor 700
FAB6127N
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Alexa Fluor 750
FAB6127S
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Alexa Fluor Plus 405
FAB6127AFP405
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Alexa Fluor Plus 488
FAB6127AFP488
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Alexa Fluor Plus 555
FAB6127AFP555
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Alexa Fluor Plus 594
FAB6127AFP594
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Alexa Fluor Plus 647
FAB6127AFP647
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Alexa Fluor Plus 680
FAB6127AFP680
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Allophycocyanin
FAB6127A
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Allophycocyanin/Cy7
FAB6127APCCY7
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Biotin
FAB6127B
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CoraFluor 1
FAB6127CL1
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DyLight 350
FAB6127D
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DyLight 405
FAB6127E
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DyLight 488
FAB6127K
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DyLight 550
FAB6127L
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DyLight 594
FAB6127M
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DyLight 650
FAB6127W
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DyLight 680
FAB6127Y
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DyLight 755
FAB6127Z
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FITC
FAB6127F
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HRP
FAB6127H
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Janelia Fluor 525
FAB6127JF525
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Janelia Fluor 549
FAB6127I
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Janelia Fluor 646
FAB6127J
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Janelia Fluor 669
FAB6127JF669
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mFluor Violet 450 SE
FAB6127MFV450
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mFluor Violet 500 SE
FAB6127MFV500
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mFluor Violet 610 SE
FAB6127MFV610
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PE/Atto594
FAB6127PEATT594
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PE/Cy5.5
FAB6127PECY55
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PE/Cy7
FAB6127PECY7
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PerCP
FAB6127C
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Recombinant Monoclonal Rat IgG2B Clone # IM7.8.1R

View all CD44 Antibodies »

Product Specifications

Immunogen

Myeloid leukemia M1 cells induced with Dexamethasone

Specificity

Detects human and mouse CD44 in flow cytometry.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Human/Mouse CD44 PE-conjugated Antibody

Detection of CD44 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of CD44 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cell (PBMCs) were stained with Rat Anti-Human/Mouse CD44 PE-conjugated Monoclonal Antibody (Catalog # FAB6127P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of CD44 antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of CD44 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Human/Mouse CD44 PE-conjugated Monoclonal Antibody (Catalog # FAB6127P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human/Mouse CD44 PE-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood mononuclear cell (PBMCs) and mouse splenocytes

Reviewed Applications

Read 1 review rated 5 using FAB6127P in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD44

CD44, also known as ECMR-III, Hermes Antigen, Pgp1, Ly-24, GP90, and HUTCH-I, is a ubiquitously expressed protein that is the major receptor for hyaluronic acid (HA). CD44 mediates cell-cell and cell-matrix interaction through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagen, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Mouse CD44 has a 22 amino acid (aa) signal sequence, an extracellular domain (ECD) with a 100 aa hyaluronan-binding disulfide-stabilized link region and a 48-463 aa stem region, a 21 aa transmembrane domain, and a 72 aa cytoplasmic domain. CD44 exists as a large number of different isoforms due to alternative RNA splicing. Clone IM7.8.1 has been reported to recognize all isoforms of CD44. In the ECD, human and mouse CD44 share about 65% aa sequence identity.

Alternate Names

CD44, ECMR-III, HCAM, HCELL, LHR, MDU2, MDU3, MIC4, MUTCH-I, Pgp1

Entrez Gene IDs

960 (Human); 12505 (Mouse); 25406 (Rat); 100126860 (Porcine)

Gene Symbol

CD44

Additional CD44 Products

Product Documents for Human/Mouse CD44 PE-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse CD44 PE-conjugated Antibody

For research use only

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