Mouse Fc gamma RII/RIII (CD32/CD16) Antibody

Detection of Fc gamma RII/RIII (CD32/CD16) by Western Blot

Inhibition of SENP6 promoted microglial polarization towards an anti-inflammatory phenotype after OGD/R treatment. A, B Primary cultured microglia were infected with adenovirus expressing wild-type SENP6 or shRNA against SENP6 following OGD/R treatment. The mRNA levels of OGD/R-induced proinflammatory (A) and anti-inflammatory (B) phenotype marker genes were detected by RT–qPCR. C Immunoblots showing the protein expression of iNOS, CD16/32, Arginase-1 and CD206 in primary microglia infected with Ad-SENP6 or Ad-sh. SENP6. D–G Quantification analysis of the protein levels in C. H–M The supernatant of primary microglia was collected, and cytokine levels were detected by ELISA. Data are presented as the mean ± S.E.M. from at least three dependent experiments and analysed by one-way ANOVA followed by Tukey’s post-hoc test. ns for P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35869493), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse Fc gamma RII/RIII (CD32/CD16) by Western Blot

Fc gammaRIIb expression in liver from Glmpgt/gt and WT mice. A-B) Fc gammaRII expression in liver sinusoids of A) 4 months old, and B) 9–10 months old Glmpgt/gt and WT male mice. In WT livers, positive immunostaining (red fluorescence) was seen along the sinusoids in the same cells that had taken up FITC-FSA (green fluorescence), while the expression was low or absent in Glmpgt/gt livers, including cells that had taken up FITC-FSA (arrows). Nuclei in A) are stained with DAPI (blue). Scale bars: 20 μm. C) Quantitative image analysis of Fc gammaRII staining (% positive area) in liver sections from WT and Glmpgt/gt mice. Groups: Young WT, 3–6 months (n = 5); young Glmpgt/gt, 4 months (n = 5); old WT, 9–10 months (n = 4), and old Glmpgt/gt, 9–10 months (n = 6). Each dot represents one animal. Medians are presented as horizontal lines, and upper and lower lines represent interquartile range. *p-value < 0.05, **p-value < 0.01, One-way non-parametric ANOVA on ranks (Kruskal-Wallis test). D) Fcgr2b expression (qPCR) in liver tissue from 4 months (“young”) and 9 months (“old”) WT and Glmpgt/gt male mice (young: n = 4 per group; old: n = 3 per group). *p-value < 0.05 (Mann Whitney U test). Error bars represent standard deviation. E, F) Western blots showing Fc gammaRII expression in whole liver lysates from E) 4 WT mice and 3 Glmpgt/gt mice, aged 4 months, and F) 4 WT mice and 3 Glmpgt/gt mice, aged 9 months, all male. LSEC: Mouse liver sinusoidal endothelial cell lysates (C57Bl/6JRj, WT). Protein loaded per lane: Liver lysates, 25 μg; LSEC, 5 μg. Beta-actin loading control was performed on the stripped blots. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37910485), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse Fc gamma RII/RIII (CD32/CD16) by Western Blot

Fc gammaRIIb expression in liver from Glmpgt/gt and WT mice. A-B) Fc gammaRII expression in liver sinusoids of A) 4 months old, and B) 9–10 months old Glmpgt/gt and WT male mice. In WT livers, positive immunostaining (red fluorescence) was seen along the sinusoids in the same cells that had taken up FITC-FSA (green fluorescence), while the expression was low or absent in Glmpgt/gt livers, including cells that had taken up FITC-FSA (arrows). Nuclei in A) are stained with DAPI (blue). Scale bars: 20 μm. C) Quantitative image analysis of Fc gammaRII staining (% positive area) in liver sections from WT and Glmpgt/gt mice. Groups: Young WT, 3–6 months (n = 5); young Glmpgt/gt, 4 months (n = 5); old WT, 9–10 months (n = 4), and old Glmpgt/gt, 9–10 months (n = 6). Each dot represents one animal. Medians are presented as horizontal lines, and upper and lower lines represent interquartile range. *p-value < 0.05, **p-value < 0.01, One-way non-parametric ANOVA on ranks (Kruskal-Wallis test). D) Fcgr2b expression (qPCR) in liver tissue from 4 months (“young”) and 9 months (“old”) WT and Glmpgt/gt male mice (young: n = 4 per group; old: n = 3 per group). *p-value < 0.05 (Mann Whitney U test). Error bars represent standard deviation. E, F) Western blots showing Fc gammaRII expression in whole liver lysates from E) 4 WT mice and 3 Glmpgt/gt mice, aged 4 months, and F) 4 WT mice and 3 Glmpgt/gt mice, aged 9 months, all male. LSEC: Mouse liver sinusoidal endothelial cell lysates (C57Bl/6JRj, WT). Protein loaded per lane: Liver lysates, 25 μg; LSEC, 5 μg. Beta-actin loading control was performed on the stripped blots. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37910485), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse Fc gamma RII/RIII (CD32/CD16) by Immunohistochemistry

Fc gammaRIIb expression in liver from Glmpgt/gt and WT mice. A-B) Fc gammaRII expression in liver sinusoids of A) 4 months old, and B) 9–10 months old Glmpgt/gt and WT male mice. In WT livers, positive immunostaining (red fluorescence) was seen along the sinusoids in the same cells that had taken up FITC-FSA (green fluorescence), while the expression was low or absent in Glmpgt/gt livers, including cells that had taken up FITC-FSA (arrows). Nuclei in A) are stained with DAPI (blue). Scale bars: 20 μm. C) Quantitative image analysis of Fc gammaRII staining (% positive area) in liver sections from WT and Glmpgt/gt mice. Groups: Young WT, 3–6 months (n = 5); young Glmpgt/gt, 4 months (n = 5); old WT, 9–10 months (n = 4), and old Glmpgt/gt, 9–10 months (n = 6). Each dot represents one animal. Medians are presented as horizontal lines, and upper and lower lines represent interquartile range. *p-value < 0.05, **p-value < 0.01, One-way non-parametric ANOVA on ranks (Kruskal-Wallis test). D) Fcgr2b expression (qPCR) in liver tissue from 4 months (“young”) and 9 months (“old”) WT and Glmpgt/gt male mice (young: n = 4 per group; old: n = 3 per group). *p-value < 0.05 (Mann Whitney U test). Error bars represent standard deviation. E, F) Western blots showing Fc gammaRII expression in whole liver lysates from E) 4 WT mice and 3 Glmpgt/gt mice, aged 4 months, and F) 4 WT mice and 3 Glmpgt/gt mice, aged 9 months, all male. LSEC: Mouse liver sinusoidal endothelial cell lysates (C57Bl/6JRj, WT). Protein loaded per lane: Liver lysates, 25 μg; LSEC, 5 μg. Beta-actin loading control was performed on the stripped blots. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37910485), licensed under a CC-BY license. Not internally tested by R&D Systems.