Mouse CCL2/JE/MCP-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF-479-NA
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse, Rat, Transgenic Mouse
Applications
Validated:
Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization
Cited:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, Bioassay, Dot Blot, ELISA Development, ELISA Development (Capture), In vivo assay, Luminex Development
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse CCL2/JE/MCP-1
Gln24-Arg96
Accession # Q5SVU3
Gln24-Arg96
Accession # Q5SVU3
Specificity
Detects mouse CCL2/JE/MCP-1 in ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse CCL2/JE/MCP-1 Antibody
Detection of Recombinant Mouse CCL2/JE/MCP‑1 by Western Blot.
Western blot shows 25 ng of Recombinant Mouse CCL2/JE/MCP-1 (479-JE), Recombinant Human CCL2/JE/MCP-1 (Catalog # 279-MC), Recombinant Rat CCL2/JE/MCP 3144-JE), Recombinant Mouse CCL11/Eotaxin (Catalog # 420-ME), Recombinant Mouse CCL12/MCP-5 (Catalog # 428-P5), and Recombinant Mouse CCL7/MCP-3/MARC (456-MC). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse CCL2/JE/MCP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-479-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for CCL2/JE/ MCP-1 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Chemotaxis Induced by CCL2/JE and Neutralization by Mouse CCL2/JE Antibody.
Recombinant Mouse CCL2/JE (Catalog # 479-JE) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL2/JE (0.04 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL2/JE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-479-NA). The ND50 is typically 0.5-2.5 µg/mL.
Mouse CCL2 / JE / MCP-1 ELISA Standard Curve
Recombinant Mouse CCL2/JE/MCP-1 (Catalog # 479-JE) was serially diluted and captured by Goat Anti-Mouse CCL2/JE/MCP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-479-NA) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CCL2/JE/MCP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-479-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Mouse CCL2/JE/MCP-1 Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse CCL2/JE/MCP-1 (Catalog # 479-JE)
Sample: Recombinant Mouse CCL2/JE/MCP-1 (Catalog # 479-JE)
Neutralization
Measured by its ability to neutralize CCL2/JE/MCP-1-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR2A. The Neutralization Dose (ND50) is typically 0.5-2.5 µg/mL in the presence of 0.04 µg/mL Recombinant Mouse CCL2/JE/MCP-1.
Mouse CCL2/JE/MCP-1 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 4 reviews rated 4.3 using AF-479-NA in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL2/JE/MCP-1
Mouse CCL2 is a member of the beta (C-C) subfamily of chemokines. The murine CCL2 gene was initially identified as a platelet-derived growth factor-inducible gene in murine fibroblasts. Mouse CCL2 cDNA encodes a 148 amino acid (aa) residue with a putative 23 aa signal peptide that is cleaved to generate the mature protein. Mouse CCL2 shares 82% amino acid sequence identity with rat CCL2. Mouse CCL2 also shares 55% amino acid sequence identity with human MCP-1. Compared to human MCP-1, mouse CCL2 has a 49 aa residue extension at the carboxy-terminus. Mouse CCL2 has full activity on human cells while human MCP-1 has limited activity on mouse cells.
Alternate Names
GDCF-2, HC11, HSMCR30, MCAF, MCP-1, SMC-CF
Gene Symbol
CCL2
UniProt
Additional CCL2/JE/MCP-1 Products
Product Documents for Mouse CCL2/JE/MCP-1 Antibody
Product Specific Notices for Mouse CCL2/JE/MCP-1 Antibody
For research use only
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