Mouse BACE-2 Ectodomain Alexa Fluor™ Plus 594-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2977AFP594
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot, Immunoprecipitation
Label
Alexa Fluor Plus 594 (Excitation = 590 nm, Emission = 618 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Specificity
Detects mouse BACE-2 Ectodomain in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 15% cross‑reactivity with recombinant mouse BACE and recombinant human BACE-2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications
Application
Recommended Usage
Immunoprecipitation
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Background: BACE-2
BACE-2 is an aspartic protease that shares sequence similarity with BACE-1, the major beta-secretase for generation of A beta peptide in neurons (1). However, BACE-2 differs from BACE-1 in several aspects including pro-enzyme activation, substrate binding sites, transcriptional regulation, and function in A beta peptide production (2‑4). The domain structure of mouse BACE-2 consists of a signal peptide (aa 1‑19), pro (aa 20‑62) and a mature chain (aa 63‑514) that consists of a transmembrane domain and a cytoplasmic tail. The C-terminal region (aa 463‑514) was replaced by a His tag in the recombinant mouse BACE-2 that also contained a His residue at the position of Tyr357. The purified recombinant mouse BACE-2 started at Ala63, the same residue shown at the N-terminus of the human active BACE-2 (2).
References
- Cai, H. et al. (2001) Nature Neurosci. 4:233.
- Hussain, I. et al. (2001) J. Biol. Chem. 276:23322.
- Ostermann, N. et al. (2006) J. Mol. Biol. 355:249.
- Sun, X. et al. (2005) FASEB J. 19:739.
Long Name
beta-site Ayloid Precursor Protein Cleaving Enzyme 2
Alternate Names
BACE2
Gene Symbol
BACE2
UniProt
Additional BACE-2 Products
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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