Mouse ACE/CD143 Antibody

Catalog # AF1513 | R&D Systems, Inc. a Bio-Techne Brand
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AF1513
AF1513-SP
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Key Product Details

Species Reactivity

Mouse

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Mouse ACE/CD143 Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse ACE/CD143
Leu35-Gln1264
Accession # P09470

Specificity

Detects mouse ACE in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human ACE is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse ACE/CD143 Antibody

Detection of Mouse ACE/CD143 antibody by Western Blot.

Detection of Mouse ACE/CD143 by Western Blot.

Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.05 µg/mL of Goat Anti-Mouse ACE/CD143 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1513) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for ACE/CD143 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
ACE/CD143 antibody in Mouse Kidney by Immunohistochemistry (IHC-Fr).

ACE/CD143 in Mouse Kidney.

ACE/CD143 was detected in perfusion fixed frozen sections of mouse kidney using 15 µg/mL Goat Anti-Mouse ACE/CD143 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1513) overnight at 4 °C. Tissue was stained (red). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Detection of Mouse ACE/CD143 antibody by Simple WesternTM.

Detection of Mouse ACE/CD143 by Simple WesternTM.

Simple Western lane view shows lysates of mouse lung tissue, loaded at 0.2 mg/mL. A specific band was detected for ACE/CD143 at approximately 218 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse ACE/CD143 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1513) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.

Applications for Mouse ACE/CD143 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Mouse lung single‑cell suspension

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse kidney

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse ACE/CD143 Somatic Form (Catalog # 1513-ZN), see our available Western blot detection antibodies

Simple Western

10 µg/mL
Sample:   Mouse lung tissue

Western Blot

0.05 µg/mL
Sample:   Mouse lung tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using AF1513 in the following applications:

Published Applications

Read 4 publications using AF1513 in the following applications:

Formulation, Preparation and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ACE/CD143

ACE (also known as peptidyl-dipetidase A) is a zinc metallopeptidase important for blood pressure control and water and salt metabolism (1). It cleaves the C-terminal dipeptide from angiotensin I to produce the potent vasopressor octapeptide angiotensin II and inactivates bradykinin by the sequential removal of two C-terminal dipeptides. In addition to the two physiological substrates, ACE cleaves C-terminal dipeptides from various oligopeptides with a free C-terminus. Because of its location and specificity, ACE plays additional roles in immunity, reproduction and neuropeptide regulation. For example, ACE degrades Alzheimer amyloid beta-peptide (A beta), retards A beta aggregation, deposition, fibril formation, and inhibits cytotoxicity (2).

ACE is a type I membrane protein and exists in two isoforms (1). Somatic ACE, found in endothelial, epithelial and neuronal cells, comprises two highly similar catalytic domains called N- and C-domains. Germinal ACE, found exclusively in the testes, comprises a single catalytic domain identical to the C-domain of somatic ACE except for an N-terminal 67 residue germinal ACE-specific sequence. Physiological functions of the two tissue-specific isozymes are not interchangeable (3). For example, sperm-specific expression of the germinal ACE, not the somatic ACE, in ACE knockout male mice restored fertility.

Soluble ACE is present in many biological fluids, such as serum, seminal fluid, amniotic fluid and cerebrospinal fluid (1). The soluble ACE is derived from the membrane forms by actions of secretases or sheddases. The identities of the secretases have not been revealed, although they belong to the family of zinc metallopeptidases (4, 5).

References

  1. Corvol, P. et al. (2004) in Handbook of Proteolytic Enzymes (Barrett, A.J. et al., eds.) p. 332, Academic Press, San Diego.
  2. Hu, J. et al. (2001) J. Biol. Chem. 276:47863.
  3. Kessler, S.P. et al. (2000) J. Biol. Chem. 275:26259.
  4. Eyries, M. et al. (2001) J. Biol. Chem. 276:5525.
  5. Alfalah, M. et al. (2001) J. Biol. Chem. 276:21105.

Long Name

Angiotensin I Converting Enzyme

Alternate Names

CD143, DCP1, Kininase II

Entrez Gene IDs

1636 (Human); 11421 (Mouse)

Gene Symbol

ACE

UniProt

P09470

Product Documents for Mouse ACE/CD143 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse ACE/CD143 Antibody

For research use only

Citations for Mouse ACE/CD143 Antibody (4)

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Customer Reviews for Mouse ACE/CD143 Antibody (1)

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  • Mouse ACE/CD143 Antibody
    Name: Kevin Schauer
    Application: Western Blot
    Sample Tested: Adult small intestine
    Species: Opsanus beta
    Verified Customer | Posted 02/15/2017
    Mouse ACE/CD143 Antibody AF1513

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FAQs for Mouse ACE/CD143 Antibody

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  • Q: Is ACE-1 the same as ACE?

    A: Yes. ACE-1 is used as an alternative name, but not as commonly as ACE and CD143.

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