Integrin alpha 5 beta 1 Antibody (SG/19) [Alexa Fluor™ Plus 488]
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43578AFP488
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Functional
Label
Alexa Fluor Plus 488
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # SG/19
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
The original antibody was generated by immunizing BALB/c mice with Nalm-6 cells. (Uniprot# P05556)
Specificity
This antibody binds human integrin alpha 5 beta 1 near the N-terminus. Epitope mapping of SG/19 revealed that Thr82 in the 1 subunit, located at the outer face of the boundary between the I-like and hybrid domains, was the key binding determinant for this antibody. Integrin alpha5 beta 1 is also known as fibronectin receptor and binds matrix macromolecules and proteinases and thereby stimulates angiogenesis. Integrin alpha5beta1, is a member of the integrin family, is a heterodimer composed by two subunits, alpha5 (ITGA5/CD49e) and beta1 (ITGB1/CD29), and both are necessary for complete biological functions. The interaction of VLA-5 with fibronectin plays an important role in regulating inflammatory cytokine production by human articular chondrocytes (From the Cell Migration Gateway ITGA5 ITGB1
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Integrin alpha 5 beta 1 Antibody (SG/19) [Alexa Fluor™ Plus 488]
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Functional
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Integrin alpha 5 beta 1
Integrins are transmembrane proteins that mediate interactions between adhesion molecules on adjacent cells and/or the extracellular matrix (ECM). Integrins have diverse roles in several biological processes including cell migration during development and wound healing, cell differentiation, and apoptosis. Their activities can also regulate the metastatic and invasive potential of tumor cells. They exist as heterodimers consisting of alpha and beta subunits. Some alpha and beta subunits exhibit specificity for one another and may be designated as a VLA (very late antigen) member. Heterodimers often preferentially bind certain cell adhesion molecules, or constituents of the ECM. Although they have no catalytic activity, integrins can be part of multimolecular signaling complexes known as focal adhesions.
Alternate Names
VLA-5
Gene Symbol
ITGA5
Additional Integrin alpha 5 beta 1 Products
Product Specific Notices for Integrin alpha 5 beta 1 Antibody (SG/19) [Alexa Fluor™ Plus 488]
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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