Integrin alpha 5 beta 1 Antibody (SG/19) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43578
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Functional
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # SG/19
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
The original antibody was generated by immunizing BALB/c mice with Nalm-6 cells. (Uniprot# P05556)
Specificity
This antibody binds human integrin alpha 5 beta 1 near the N-terminus. Epitope mapping of SG/19 revealed that Thr82 in the 1 subunit, located at the outer face of the boundary between the I-like and hybrid domains, was the key binding determinant for this antibody. Integrin alpha5 beta 1 is also known as fibronectin receptor and binds matrix macromolecules and proteinases and thereby stimulates angiogenesis. Integrin alpha5beta1, is a member of the integrin family, is a heterodimer composed by two subunits, alpha5 (ITGA5/CD49e) and beta1 (ITGB1/CD29), and both are necessary for complete biological functions. The interaction of VLA-5 with fibronectin plays an important role in regulating inflammatory cytokine production by human articular chondrocytes (From the Cell Migration Gateway ITGA5 ITGB1
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
Novus Biologicals Mouse Integrin alpha 5 beta 1 Antibody (SG/19) - BSA Free (NBP3-43522) is a recombinant monoclonal antibody validated for use in Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Integrin alpha 5 beta 1 Antibody (SG/19) - BSA Free
Immunocytochemistry /Immunofluorescence: Integrin alpha 5 beta 1 Antibody (SG/19) - BSA Free [NBP3-43578] -
Immunocytochemistry /Immunofluorescence: Integrin alpha 5 beta 1 Antibody (SG/19) - BSA Free [NBP3-43578] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells on Shi-fix coverslips stained with NBP3-43578 (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom: NBP3-43578, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.Applications for Integrin alpha 5 beta 1 Antibody (SG/19) - BSA Free
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Functional
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Integrin alpha 5 beta 1
Integrins are transmembrane proteins that mediate interactions between adhesion molecules on adjacent cells and/or the extracellular matrix (ECM). Integrins have diverse roles in several biological processes including cell migration during development and wound healing, cell differentiation, and apoptosis. Their activities can also regulate the metastatic and invasive potential of tumor cells. They exist as heterodimers consisting of alpha and beta subunits. Some alpha and beta subunits exhibit specificity for one another and may be designated as a VLA (very late antigen) member. Heterodimers often preferentially bind certain cell adhesion molecules, or constituents of the ECM. Although they have no catalytic activity, integrins can be part of multimolecular signaling complexes known as focal adhesions.
Alternate Names
VLA-5
Gene Symbol
ITGA5
Additional Integrin alpha 5 beta 1 Products
Product Documents for Integrin alpha 5 beta 1 Antibody (SG/19) - BSA Free
Product Specific Notices for Integrin alpha 5 beta 1 Antibody (SG/19) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...