Human TRAILR1/TNFRSF10A APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB347A
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry
Cited:
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 69036
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human TRAIL R1/TNFRSF10A
Ala24-Asn239
Accession # AAC51226
Ala24-Asn239
Accession # AAC51226
Specificity
Detects recombinant human TRAIL R1/TNFRSF10A in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) TRAIL R2, rhTRAIL R3, rhTRAIL R4, or rhDcR3 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human TRAILR1/TNFRSF10A APC-conjugated Antibody
Detection of TRAIL R1/TNFRSF10A in HeLa Human Cell Line by Flow Cytometry.
HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human TRAIL R1/TNFRSF10A APC-conjugated Monoclonal Antibody (Catalog # FAB347A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications for Human TRAILR1/TNFRSF10A APC-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: HeLa human cervical epithelial carcinoma cell line
Sample: HeLa human cervical epithelial carcinoma cell line
Reviewed Applications
Read 1 review rated 5 using FAB347A in the following applications:
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
For liquid material, refer to CoA for concentration.
Formulation
Supplied in a saline solution containing BSA and Sodium Azide. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TRAILR1/TNFRSF10A
Human TRAIL R1, also called DR4, is a type 1, TNF R family, membrane protein which is a receptor for TRAIL (APO2 ligand). In the TNF superfamily nomenclature, TRAIL R1 is referred to as TNFRSF10A. TRAIL R1 cDNA encodes a 468 amino acid precursor protein containing extracellular cysteine-rich domains, a transmembrane domain and a cytoplasmic death domain. Among the TNF receptor family proteins, TRAIL R1 is most closely related to TRAIL R2/DR5, sharing 55% amino acid sequence identity. Binding of trimeric TRAIL to TRAIL R1 induces apoptosis. The induction of apoptosis likely requires oligomerization of the receptor. The human TRAIL R1/Fc chimera neutralizes the ability of TRAIL to induce apoptosis. Besides TRAIL R1, an additional TRAIL R2/DR5, which tranduces apoptosis signal, and two TRAIL decoy receptors, which antagonize TRAIL-induced apoptosis, have been reported.
References
- Pan, G. et al. (1997) Science 276:111.
- Golstein, P. (1997) Curr. Biol. 7:R750.
Long Name
TRAIL Receptor 1
Alternate Names
CD261, DR4, TNFRSF10A, TRAIL R1
Entrez Gene IDs
8797 (Human)
Gene Symbol
TNFRSF10A
UniProt
Additional TRAILR1/TNFRSF10A Products
Product Documents for Human TRAILR1/TNFRSF10A APC-conjugated Antibody
Product Specific Notices for Human TRAILR1/TNFRSF10A APC-conjugated Antibody
For research use only
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