Western blot shows lysates of SK-Mel-28 human malignant melanoma cell line and T47D human breast cancer cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human TMEM87A Monoclonal Antibody (Catalog # MAB7966) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for TMEM87A at approximately 90-95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of TMEM-87A in PC‑3 Human Cell Line by Flow Cytometry.

PC-3 human prostate cancer cell line was stained with Mouse Anti-Human TMEM87A Monoclonal Antibody (Catalog # MAB7966, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Detection of Human TMEM87A by Simple Western^TM.

Simple Western lane view shows lysate of T47D human breast cancer cell line, loaded at 0.5 mg/mL. Specific bands were detected for TMEM87A at approximately 98 kDa (as indicated) using 2 µg/mL of Mouse Anti-Human TMEM87A Monoclonal Antibody (Catalog # MAB7966). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human TMEM87A Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: PC-3 human prostate cancer cell line

Simple Western

2 µg/mL
Sample: T47D human breast cancer cell line

Western Blot

0.2 µg/mL
Sample: SK‑Mel‑28 human malignant melanoma cell line and T47D human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TMEM87A

TMEM87A (Transmembrane protein 87A) is predicted to be a 6-transmembrane, 555 amino acid (aa) glyco- and phospho-protein that is expressed on the cell surface. Its expression in the mammary gland is upregulated when there is a loss of caveolin-1 expression, which may confer susceptibility to breast cancer. Within the region used as an immunogen, human TMEM87A shares 83% aa sequence identity with mouse and rat TMEM87A. A potential isoform of 181 aa diverges C-terminal to aa 169, while another of 494 aa is divergent N-terminal to aa 70.

Long Name

Transmembrane Protein 87A

Alternate Names

FLJ11273, MGC33727

Entrez Gene IDs

25963 (Human); 211499 (Mouse); 366170 (Rat)

Gene Symbol

TMEM87A

UniProt

Q8NBN3

Additional TMEM87A Products

Product Documents for Human TMEM87A Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human TMEM87A Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human TMEM87A Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB7966

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all TMEM87A Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human TMEM87A Antibody

Detection of Human TMEM87A by Western Blot.