Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Ramos human Burkitt's lymphoma cell line, MCF-7 human breast cancer cell line, and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human TAK1 Monoclonal Antibody (Catalog # MAB5307) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TAK1 at approximately 80 to 82 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human TAK1 Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Raji human Burkitt's lymphoma cell line, Ramos human Burkitt's lymphoma cell line, MCF-7 human breast cancer cell line, and U937 human histiocytic lymphoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TAK1

TGF-beta-activated kinase 1 (TAK1; also MAP3K7) is a member of the STE Ser/Thr protein kinase family and the MAP kinase kinase kinase subfamily. Human TAK1 is 606 amino acids (aa) in length and contains a protein kinase domain at aa 36‑291. There are three isoforms for TAK1. Isoform 1B is the largest isoform. Isoform 1A has a 27 aa deletion corresponding to aa 404‑430 in isoform 1B, and isoform 1C has an 88 aa deletion corresponding to aa 519‑606 in isoform 1B. TAK1 activity has been implicated during TGF beta-induced and Ras-dependent tumorigenesis.

Long Name

TGF-beta activated kinase 1

Alternate Names

MAP3K7, TGF1a

Entrez Gene IDs

6885 (Human); 26409 (Mouse); 313121 (Rat)

Gene Symbol

MAP3K7

UniProt

Q5U0D0

Additional TAK1 Products

Product Documents for Human TAK1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human TAK1 Antibody

For research use only

Citations
Reviews

Human TAK1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5307

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all TAK1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human TAK1 Antibody

Detection of Human TAK1 by Western Blot.