Human ST2/IL-33R Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB523

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MAB523-100
MAB523-500
MAB523-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry, AlphaLISA, Mass Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 97203

View all ST2/IL-33R Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human ST2/IL-33 R
Lys19-Phe328
Accession # BAA02233

Specificity

Detects human ST2/IL-33 R in ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse ST2L, recombinant human (rh) IL-1 RI, rhIL-1 RII, rhIL-1 RA, rhIL-1 alpha, or rhIL-1 beta is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human ST2/IL-33R Antibody

IFN-gamma Secretion Induced by IL‑33 and Neutralization by Human ST2/IL-33 R Antibody.

IFN-gamma Secretion Induced by IL‑33 and Neutralization by Human ST2/IL-33 R Antibody.

Recombinant Human IL-33 induces IFN-gamma secretion in Human Peripheral Blood Mononuclear cells (PBMC) in the presence of Recombinant Human IL-12 (Catalog # 219-IL) in a dose-dependent manner (orange line), as measured by the Human IFN-gamma Quantikine ELISA Kit (Catalog # DIF50C). Under these conditions, IFN-gamma secretion elicited by IL-33 is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Primate ST2/IL-33 R Monoclonal Antibody (Catalog # MAB523). The ND50 is typically 0.3-1.5 µg/mL.
Detection of Human ST2/IL-33R by Immunocytochemistry/Immunofluorescence

Detection of Human ST2/IL-33R by Immunocytochemistry/Immunofluorescence

Immunolocalization of ST2 in colonic tissue from responder and non-responder patients during baseline and 6-month follow-up. a Total ST2 immunoreactivity was restricted to the cellular infiltrate in the lamina propria at 6 months in responder patients (right), while in patients showing reactivation, total ST2 was increased in inflamed mucosa, also confined to the cellular infiltrate (left); baseline examination revealed extensive immune cell infiltration of the intestinal mucosa and damaged tissue with loss of architecture. b Total ST2 immunoreactivity at 6 months expressed as arbitrary units (A.U.) and normalized to baseline levels in responders and non-responders (n = 4 in each group) was higher in the non-responders. Hoechst 33342/ ST2 (blue/green) (60X) Image collected and cropped by CiteAb from the following publication (https://bmcgastroenterol.biomedcentral.com/articles/10.1186/s12876-016-…), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human ST2/IL-33R Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human ST2/IL-1 R4 Fc Chimera (Catalog # 523-ST)

Neutralization

Measured by its ability to neutralize IL‑33-induced IFN-gamma secretion in Human Peripheral Blood Mononuclear cells (PBMC).The Neutralization Dose (ND50) is typically 0.3-1.5 μg/mL in the presence of 1 ng/mL Recombinant Human IL‑33.

Human/Primate ST2/IL-33 R Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human/Primate ST2/IL-33R Biotinylated Antibody (Catalog # BAF523)
  • Standard: Recombinant Human ST2/IL-33R Fc Chimera Protein, CF (Catalog # 523-ST)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ST2/IL-33R

ST2, also known as IL-33R, IL-1 R4 and T1, is an Interleukin-1 receptor family glycoprotein that contributes to Th2 immune responses (1, 2). Human ST2 consists of a 310 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 21 aa transmembrane segment, and a 207 aa cytoplasmic domain with an intracellular TIR domain (3, 4). Alternate splicing of the 120 kDa human ST2 generates a soluble 60 kDa isoform that lacks the transmembrane and cytoplasmic regions as well as an isoform that additionally lacks the third Ig‑like domain (4). Within the ECD, human ST2 shares 68% and 64% aa sequence identity with mouse and rat ST2, respectively. ST2 is expressed on the surface of mast cells, activated Th2 cells, macrophages, and cardiac myocytes (5‑8). It binds IL-33, a cytokine that is upregulated by inflammation or mechanical strain in smooth muscle cells, airway epithelia, keratinocytes, and cardiac fibroblasts (5, 9). IL-33 binding induces the association of ST2 with IL-1R AcP, a shared signaling subunit that also associates with IL-1 RI and IL-1 R rp2 (1, 10, 11). In macrophages, ST2 interferes with signaling from IL-1 RI and TLR4 by sequestering the adaptor proteins MyD88 and Mal (7). In addition to its role in promoting mast cell and Th2 dependent inflammation, ST2 activation enhances antigen induced hypernociception and protects from atherosclerosis and cardiac hypertrophy (5, 12‑14). The soluble ST2 isoform is released by activated Th2 cells and strained cardiac myocytes and is elevated in the serum in allergic asthma (6, 8, 15). Soluble ST2 functions as a decoy receptor that blocks IL‑33's ability to signal through transmembrane ST2 (10, 13‑15).

References

  1. Barksby, H.E. et al. (2007) Clin. Exp. Immunol. 149:217.
  2. Gadina, M. and C.A. Jefferies (2007) Science STKE 2007:pe31.
  3. Tominaga, S. et al. (1992) Biochim. Biophys. Acta 1171:215.
  4. Li, H. et al. (2000) Genomics 67:284.
  5. Schmitz, J. et al. (2005) Immunity 23:479.

  6. Lecart, S. et al. (2002) Eur. J. Immunol. 32:2979.
  7. Brint, E.K. et al. (2004) Nat. Immunol. 5:373.
  8. Weinberg, E.O. et al. (2002) Circulation 106:2961.
  9. Sanada S. et al. (2007) J. Clin. Invest. 117:1538.

  10. Palmer, G. et al. (2008) Cytokine 42:358.
  11. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
  12. Allakhverdi, Z. et al. (2007) J. Immunol. 179:2051.
  13. Verri Jr., W.A. et al. (2008) Proc. Natl. Acad. Sci. 105:2723.
  14. Miller, A.M. et al. (2008) J. Exp. Med. 205:339.
  15. Hayakawa, H. et al. (2007) J. Biol. Chem. 282:26369.

Long Name

Interleukin 33 Receptor

Alternate Names

DER4, Fit-1, IL-1 R4, IL-1R4, IL-1RL1, IL-33 R, IL-33R, IL1R4, IL33R, Ly84, ST2L, ST2V, T1

Entrez Gene IDs

9173 (Human); 17082 (Mouse)

Gene Symbol

IL1RL1

UniProt

BAA02233

Additional ST2/IL-33R Products

Product Documents for Human ST2/IL-33R Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human ST2/IL-33R Antibody

For research use only

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