Human Somatostatin R2/SSTR2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4224
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Primate
Applications
Validated:
Immunohistochemistry, Intracellular Staining by Flow Cytometry, Immunocytochemistry, CyTOF-ready
Cited:
Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry, Assay Development
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 402038
Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with Somatostatin R2/SSTR2
Met1-Ile369
Accession # P30874
Met1-Ile369
Accession # P30874
Specificity
Detects human Somatostatin R2/SSTR2. Stains SSTR2 transfectants but not irrelevant transfectants.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Somatostatin R2/SSTR2 Antibody
Somatostatin R2/SSTR2 in MDA-MB-231 Human Cell Line.
Somatostatin R2/SSTR2 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using 10 µg/mL Human Somatostatin R2/SSTR2 Monoclonal Antibody (Catalog # MAB4224) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (brown; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Somatostatin R2/SSTR2 in MDA-MB-231 Human Cell Line by Flow Cytometry.
MDA-MB-231 human breast cancer cell line was stained with Human Somatostatin R2/SSTR2 Monoclonal Antibody (Catalog # MAB4224, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Somatostatin R2/SSTR2 in Human Brain.
Somatostatin R2/ SSTR2 was detected in immersion fixed paraffin-embedded sections of human brain (cingulate cortex) using 3 µg/mL Human Somatostatin R2/SSTR2 Monoclonal Antibody (Catalog # MAB4224) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Somatostatin R2/SSTR2 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed MDA-MB-231 human breast cancer cell line
Sample: Immersion fixed MDA-MB-231 human breast cancer cell line
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cingulate cortex)
Sample: Immersion fixed paraffin-embedded sections of human brain (cingulate cortex)
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Sample: MDA-MB-231 human breast cancer cell line fixed with paraformaldehyde and permeabilized with saponin
Sample: MDA-MB-231 human breast cancer cell line fixed with paraformaldehyde and permeabilized with saponin
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Somatostatin R2/SSTR2
Somatostatin Receptor 2 (SSTR2) is one of five 7-transmembrane G-protein-coupled receptors for somatostatins 14 and 28. Human SSTR2 shares 84% aa identity with mouse SSTR2 within the extracellular domains. Isoform B (357 aa) has an alternate C-terminal cytoplasmic region that is 12 aa shorter than that of isoform A (369 aa, reported as 93 kDa). Both are expressed in brain, stomach, intestinal epithelia, pancreatic islets and kidney tubules. Isoform B is also expressed in parotid, thyroid and bronchial glands.
Long Name
Somatostatin Receptor Type 2
Alternate Names
Smstr2, SomatostatinR2, SST2, SSTR2
Gene Symbol
SSTR2
UniProt
Additional Somatostatin R2/SSTR2 Products
Product Documents for Human Somatostatin R2/SSTR2 Antibody
Product Specific Notices for Human Somatostatin R2/SSTR2 Antibody
For research use only
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