Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and NRK rat normal kidney cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Rat Tollip Monoclonal Antibody (Catalog # MAB4678) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Tollip at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Human/Rat Tollip Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and NRK rat normal kidney cells

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tollip

Tollip (Toll-interacting protein) is a 28 kDa, cytoplasmic protein that is involved in IL-1 and Toll receptor signaling. It is classified as an adaptor protein that performs multiple functions. It binds both IRAK and Toll receptors, inhibiting constitutive IL-1 and Toll receptor signaling. It also binds to ubiquinated IL-1 RI, directing it to the endosomal degradation compartment. Human Tollip is 274 amino acids(aa) in length. It contains a C2 Ca⁺⁺-binding region (aa 54‑154) and a CUE domain (aa 229‑270) that binds ubiquitin. One potential splice form exists that shows a deletion of aa 6‑33. Human Tollip is 92% identical to mouse Tollip.

Long Name

Toll Interacting Protein

Alternate Names

IL-1RAcPIP

Entrez Gene IDs

54472 (Human); 54473 (Mouse); 361677 (Rat)

Gene Symbol

TOLLIP

UniProt

Q9H0E2 (Human)

Additional Tollip Products

Product Documents for Human/Rat Tollip Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Rat Tollip Antibody

For research use only

Citations
Reviews

Human/Rat Tollip Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4678

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Tollip Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Rat Tollip Antibody

Detection of Human/Rat Tollip by Western Blot.