Protein O-Glucosyltransferase 1/POGLUT was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Protein O-Glucosyltransferase 1/POGLUT Monoclonal Antibody (Catalog # MAB64371) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Protein O-Glucosyltransferase 1/POGLUT1 Antibody

Application

Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Protein O-Glucosyltransferase 1/POGLUT1

Protein O-Glucosyltransferase1 (POGLUT1) is a homologue of Rumi from Drosophila, an endoplasmic reticulum (ER)-retaining glucosyltransferase, that adds glucose to serine residues within the consensus sequence of C1‑X‑S‑X‑P‑C2 in Notch EGF repeats, thereby regulating cell-fate decisions (1). It is also known as CAP10-like protein (2) and KTELC1 due to the highly conserved CAP10 domain and the presence of an ER-retaining motif, KTEL, at the C‑terminus. The human gene is reported to be involved in the pathogenesis of both acute myelogenous and T‑acute lymphoblastic leukemias (3). POGLUT1 has hydrolase activity on UDP-Glc in a phosphatase-coupled glycosyltransferase assay (4).

References

Acar, M. et al. (2008) Cell 132:247.
Teng, Y. et al. (2006) Gene 371:7
Wang, Y. et al. (2010) Genet Test Mol Biomarkers 14:127.
Wu, Z.L. et al. (2011) Glycobiology 21:727.

Alternate Names

CLP46, KDELCL1, KTELC1, MDS010, MDSRP, POGLUT1, Rumi

Entrez Gene IDs

56983 (Human)

Gene Symbol

POGLUT1

UniProt

Q8NBL1

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Product Documents for Human Protein O-Glucosyltransferase 1/POGLUT1 Antibody

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COA

SDS

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Product Specific Notices for Human Protein O-Glucosyltransferase 1/POGLUT1 Antibody

For research use only

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Human Protein O-Glucosyltransferase 1/POGLUT1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB64371

Key Product Details

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Product Specifications

Immunogen

Specificity

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Scientific Data Images for Human Protein O-Glucosyltransferase 1/POGLUT1 Antibody

Protein O‑Glucosyltransferase 1/POGLUT in Human Liver.