Detects human Podocalyxin in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse Podocalyxin or recombinant human Endoglycan is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Scientific Data Images for Human Podocalyxin PE-conjugated Antibody

Detection of Podocalyxin in MDA-MB-231 human cell line by Flow Cytometry.

MDA-MB-231 (orange) or MG-63 (blue) cell lines were stained with Mouse Anti-Human Podocalyxin PE-conjugated Monoclonal Antibody (Catalog # FAB1658P, filled histograms) or isotype control antibody (Catalog # IC003P, open histograms). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Podocalyxin PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

2.5 µg/mL
Sample: MDA-MB-231 Human Cell Line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Podocalyxin

Podocalyxin, also known as Podocalyxin-like protein-1 (PCLP1 or PODXL), is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology (1). Podocalyxin is a major sialoprotein in the podocytes of the kidney glomerulus and is also expressed by both endothelium and multipotent hematopoietic progenitors (2). It has been identified as a novel cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells.

References

Li, J. et al. (2001) DNA Seq. 12:407.
Hara, T. et al. (1999) Immunity 11:567.

Alternate Names

GCTM, PCLP, POD XL, PODXL

Entrez Gene IDs

5420 (Human); 27205 (Mouse)

Gene Symbol

PODXL

UniProt

AAB61574

Additional Podocalyxin Products

Product Documents for Human Podocalyxin PE-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Podocalyxin PE-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human Podocalyxin PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB1658P

Key Product Details

Species Reactivity

Validated:

Cited:

Applications