PINK1 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 15 µg/mL Rat Anti-Human PINK1 Monoclonal Antibody (Catalog # MAB4357) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human PINK1 Antibody

Application

Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex) subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PINK1

PTEN-induced putative kinase 1 (PINK1) is a ubiquitous protein with highest levels in heart, skeletal muscle, and testis, and with lower expression in other tissues, including brain. PINK1 contains a mitochondrial targeting motif and a highly conserved protein kinase domain with strong homology to serine/threonine kinases of the calcium/calmodulin family. Mutations in the Pink1 gene have been identified as a cause of autosomal recessive early-onset parkinsonism.

Long Name

PTEN-induced Putative Kinase 1

Alternate Names

BRPK, PARK6

Entrez Gene IDs

65018 (Human); 68943 (Mouse); 298575 (Rat); 3925520 (T. castaneum)

Gene Symbol

PINK1

UniProt

ABM87645

Additional PINK1 Products

Product Documents for Human PINK1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human PINK1 Antibody

For research use only

Citations
Reviews

Human PINK1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4357

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all PINK1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human PINK1 Antibody

PINK1 in Human Brain.