Human p53R2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3788
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human p53R2
Met1-Lys180
Accession # Q7LG56
Met1-Lys180
Accession # Q7LG56
Specificity
Detects human p53R2 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human p53R2 Antibody
Detection of Human p53R2 by Western Blot.
Western blot shows lysates of HEK293 human embryonic kidney cell line and U2OS human osteosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Human p53R2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3788) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for p53R2 at approximately 45 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
Detection of p53R2 by Western Blot
Gemcitabine resistance is driven by RRM1 overexpression. A) Quantification of relative RRM1 mRNA abundance following shRNA-mediated knock-down in BxPC3, BxGR-80C and BxGR-360C cells. Three independent RNA samples analyzed per cell type. b) Relative gemcitabine IC50 values following RRM1 knock-down, normalized to the control for each subclone. c) Correlation between relative RRM1 mRNA abundance and absolute gemcitabine (GEM) IC50 for all RRM1 knock-down cells and controls. d) Western blotting of dose-dependent induction of an RRM1 band with the apparent mass of 110–120 kDa (RRM1*) and RRM2 in gemcitabine treated cells. a-b) Statistical significance tested using one-way ANOVA with Tukey’s multiple comparison test. Error-bars indicate standard error of the mean. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35617275), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of p53R2 by Western Blot
Gemcitabine resistance is driven by RRM1 overexpression. A) Quantification of relative RRM1 mRNA abundance following shRNA-mediated knock-down in BxPC3, BxGR-80C and BxGR-360C cells. Three independent RNA samples analyzed per cell type. b) Relative gemcitabine IC50 values following RRM1 knock-down, normalized to the control for each subclone. c) Correlation between relative RRM1 mRNA abundance and absolute gemcitabine (GEM) IC50 for all RRM1 knock-down cells and controls. d) Western blotting of dose-dependent induction of an RRM1 band with the apparent mass of 110–120 kDa (RRM1*) and RRM2 in gemcitabine treated cells. a-b) Statistical significance tested using one-way ANOVA with Tukey’s multiple comparison test. Error-bars indicate standard error of the mean. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35617275), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human p53R2 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HEK293 human embryonic kidney cell line and U2OS human osteosarcoma cell line
Sample: HEK293 human embryonic kidney cell line and U2OS human osteosarcoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: p53R2
The p53-inducible ribonucleotide reductase (p53R2) shares similarity with the R2 subunit of ribonucleotide reductase. Both function to provide dNTPs for DNA synthesis. p53R2 is induced in response to genotoxic stress, whereas the R2 functions under homeostatic conditions. When the expression of p53R2 is decreased, cells exhibit decreased ribonucleotide reductase activity, DNA repair activity, and cellular survival in response to genotoxins.
Alternate Names
RRM2B
Gene Symbol
RRM2B
UniProt
Additional p53R2 Products
Product Documents for Human p53R2 Antibody
Product Specific Notices for Human p53R2 Antibody
For research use only
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