Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, and CH-1 mouse B cell lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse Vav-1 Monoclonal Antibody (Catalog # MAB3786) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Vav-1 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 10.

Applications for Human/Mouse Vav-1 Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Raji human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, and CH‑1 mouse B cell lymphoma cell line

Reviewed Applications

Read 1 review rated 2 using MAB3786 in the following applications:

Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Vav-1

The Vav-1 proto-oncogene is a guanine nucleotide exchange factor (GEF) for the low molecular weight GTPases of the Rho/Rac family. Vav-1 promotes intracellular signaling by its multiple protein binding motifs. It is a cytosolic protein primarily expressed in hematopoietic cells and plays an essential role in the proliferation and activation of T and B cells.

Alternate Names

vav-T, Vav1

Entrez Gene IDs

7409 (Human); 22324 (Mouse); 25156 (Rat)

Gene Symbol

VAV1

UniProt

P15498

Additional Vav-1 Products

Product Documents for Human/Mouse Vav-1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse Vav-1 Antibody

For research use only

Citations
Reviews

Human/Mouse Vav-1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3786

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Vav-1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse Vav-1 Antibody

Detection of Human and Mouse Vav‑1 by Western Blot.