Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Canine, Equine, Feline, Primate - Macaca mulatta (Rhesus Macaque)

Applications

Validated:

Flow Cytometry, Immunocytochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, Immunocytochemistry, Differentiation, IF/ICC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Clone # MC-813-70

View all SSEA-4 Antibodies »

Product Specifications

Immunogen

2120Ep human embryonal carcinoma cell line

Specificity

Recognizes a carbohydrate epitope of SSEA-4 (1, 2).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Scientific Data Images for Human/Mouse SSEA-4 Antibody

Detection of SSEA-4 antibody in NTera-2 Human Cell Line antibody by Flow Cytometry.

Detection of SSEA‑4 in NTera‑2 Human Cell Line by Flow Cytometry.

NTera-2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/Mouse SSEA-4 Monoclonal Antibody (Catalog # MAB1435, filled histogram) or isotype control antibody (Catalog # MAB007, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
SSEA-4 antibody in BG01V Human Stem Cells by Immunocytochemistry (ICC).

SSEA-4 in BG01V Human Stem Cells.

SSEA-4 was detected in immersion fixed BG01V human embryonic stem cells cultured on irradiated mouse embryonic fibroblasts (Catalog # PSC001) using 10 µg/mL Mouse Anti-Human/Mouse SSEA-4 Monoclonal Antibody (Catalog # MAB1435) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Canine SSEA-4 by Flow Cytometry

Detection of Canine SSEA-4 by Flow Cytometry

Flow cytometry. Comparison of cell surface proteins CD29, CD44, CD90, CD34, CD45, SSEA-1, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 on primary cultures of BM-MSCs (A, C) and AT-MSCs (B, D). Solid histograms show nonspecific staining and open histograms show specific staining for the indicated marker. Three different donor MSC populations from each tissue type were analyzed and representative samples are shown. Image collected and cropped by CiteAb from the following publication (https://bmcvetres.biomedcentral.com/articles/10.1186/1746-6148-8-150), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Applications for Human/Mouse SSEA-4 Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: NTera-2 human testicular embryonic carcinoma cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells cultured on irradiated mouse embryonic fibroblasts (Catalog # PSC001)

Reviewed Applications

Read 9 reviews rated 4.2 using MAB1435 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in MES with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SSEA-4

SSEA-4 is expressed on the surface of human embryonal carcinoma (EC) cells (the pluripotent stem cells of teratocarcinomas), human embryonic germ cells (EG), and human embryonic stem cells (ES). Expression of SSEA-4 is down-regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression (1-4).

References

  1. Shevinsky, L.H. et al. (1982) Cell 30:697.
  2. Kannagi, R. et al. (1983) EMBO J. 2:2355.
  3. Thomson, J.A. and J.S. Odorico (2000) Trends Biotechnol. 18:53.
  4. Draper, J.S. et al. (2002) J. Anat. 200:249.

Long Name

Stage-specific Embryonic Antigen-4

Alternate Names

SSEA4

Additional SSEA-4 Products

Product Documents for Human/Mouse SSEA-4 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse SSEA-4 Antibody

For research use only

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