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Human/Mouse SSEA‑4 Fluorescein‑conjugated Antibody
Catalog # FAB1435F | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Human, Mouse
Applications
Flow Cytometry
Label
Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)
Antibody Source
Monoclonal Mouse IgG3 Clone # MC-813-70
Product Summary for Human/Mouse SSEA‑4 Fluorescein‑conjugated Antibody
Immunogen
2120Ep human embryonal carcinoma cell line
Specificity
Recognizes a carbohydrate epitope of SSEA-4 (1, 2).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG3
Scientific Data Images for Human/Mouse SSEA‑4 Fluorescein‑conjugated Antibody
Detection of SSEA‑4 in NTera‑2 human testicular embryonic carcinoma cell line by Flow Cytometry.
NTera-2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/Mouse SSEA-4 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1435F, filled histogram) or isotype control antibody (Catalog # IC007F, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications for Human/Mouse SSEA‑4 Fluorescein‑conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: NTera‑2 human testicular embryonic carcinoma cell line
Sample: NTera‑2 human testicular embryonic carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Published Applications
Read 5 publications using FAB1435F in the following applications:
Formulation, Preparation and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: SSEA-4
SSEA-4 is expressed on the surface of human embryonal carcinoma (EC) cells (the pluripotent stem cells of teratocarcinomas), human embryonic germ cells (EG), and human embryonic stem cells (ES). Expression of SSEA-4 is down-regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression (1-4).
References
- Shevinsky, L.H. et al. (1982) Cell 30:697.
- Kannagi, R. et al. (1983) EMBO J. 2:2355.
- Thomson, J.A. and J.S. Odorico (2000) Trends Biotechnol. 18:53.
- Draper, J.S. et al. (2002) J. Anat. 200:249.
Long Name
Stage-specific Embryonic Antigen-4
Alternate Names
SSEA4
Product Specific Notices for Human/Mouse SSEA‑4 Fluorescein‑conjugated Antibody
For research use only
Citations for Human/Mouse SSEA‑4 Fluorescein‑conjugated Antibody (5)
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Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse SSEA‑4 Fluorescein‑conjugated Antibody include:
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Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryN Malerba et al. (2019-08-22), Generation of the induced human pluripotent stem cell lines CSSi009-A from a patient with a GNB5 pathogenic variant, and CSSi010-A from a CRISPR/Cas9 engineered GNB5 knock-out human cell line Stem Cell Res, 2019-040(0):101547.
PMID: 31479876 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryK Matsuura et al. (2019-04-25), Adequate taylor couette flow-mediated shear stress is useful for dissociating human iPS cell-derived cell aggregates Regen Ther, 2019-012(0):6-13.
PMID: 31890761 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryB Ermon et al. (2018-09-06), Derivation of human induced pluripotent stem cell line EURACi004-A from skin fibroblasts of a patient with Arrhythmogenic Cardiomyopathy carrying the heterozygous PKP2 mutation c.2569_3018del50 Stem Cell Res, 2018-032(0):78-82.
PMID: 30219716 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryV Meraviglia et al. (2017-12-18), Generation of human induced pluripotent stem cells (EURACi001-A, EURACi002-A, EURACi003-A) from peripheral blood mononuclear cells of three patients carrying mutations in the CAV3 gene Stem Cell Res, 2017-127(0):25-29.
PMID: 29304398 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryDi Maggio N et al. (2012-07-01), Fibroblast growth factor-2 maintains a niche-dependent population of self-renewing highly potent non-adherent mesenchymal progenitors through FGFR2c. Stem Cells, 2012-030(7):1455-64.
PMID: 22495904
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