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Human/Mouse/Rat GAPDH Antibody
Catalog # AF5718 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunohistochemistry, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Summary for Human/Mouse/Rat GAPDH Antibody
Immunogen
E. coli-derived recombinant human GAPDH/G3PDH
Met1-Ala150
Accession # P04406
Met1-Ala150
Accession # P04406
Specificity
Detects human, mouse, and rat GAPDH/G3PDH in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human GAPDH-2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat GAPDH Antibody
Detection of Human/Mouse/Rat GAPDH/G3PDH by Western Blot.
Western blot shows lysates of mouse, human, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat GAPDH/G3PDH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5718) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GAPDH/G3PDH at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
GAPDH in HeLa Human Cell Line.
GAPDH was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse/Rat GAPDH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5718) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
GAPDH/G3PDH in Human Kidney.
GAPDH/G3PDH was detected in immersion fixed paraffin-embedded sections of normal human kidney using Goat Anti-Human/Mouse/Rat GAPDH/G3PDH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5718) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei of convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.This application has not been tested in mouse or rat samples.Applications for Human/Mouse/Rat GAPDH Antibody
Application
Recommended Usage
Immunocytochemistry
5-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of normal human kidney and human prostate cancer tissue
Sample: Immersion fixed paraffin-embedded sections of normal human kidney and human prostate cancer tissue
Simple Western
10 µg/mL
Sample: Human brain (motor cortex) tissue and HepG2 human hepatocellular carcinoma cell line
Sample: Human brain (motor cortex) tissue and HepG2 human hepatocellular carcinoma cell line
Western Blot
1 µg/mL
Sample: Mouse, human, and rat brain tissue
Sample: Mouse, human, and rat brain tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 9 reviews rated 4.9 using AF5718 in the following applications:
Published Applications
Read 24 publications using AF5718 in the following applications:
Formulation, Preparation and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GAPDH
Product Documents for Human/Mouse/Rat GAPDH Antibody
Product Specific Notices for Human/Mouse/Rat GAPDH Antibody
For research use only
Citations for Human/Mouse/Rat GAPDH Antibody (24)
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Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse/Rat GAPDH Antibody include:
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Species: Mouse
Sample Types: Whole Tissue
Applications: Western BlotCJ Chen et al. (2022-08-15), Metformin Mitigated Obesity-Driven Cancer Aggressiveness in Tumor-Bearing Mice International Journal of Molecular Sciences, 2022-023(16).
PMID: 36012397 -
Species: Mouse,Human
Sample Types: Cell Lysates
Applications: Western BlotW Xiao et al. (2022-03-14), Robo4 is constitutively shed by ADAMs from endothelial cells and the shed Robo4 functions to inhibit Slit3-induced angiogenesis Scientific Reports, 2022-012(1):4352.
PMID: 35288626 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryY Basmaeil et al. (2021-12-10), Decidua Parietalis Mesenchymal Stem/Stromal Cells and Their Secretome Diminish the Oncogenic Properties of MDA231 Cells In Vitro Cells, 2021-110(12).
PMID: 34944000 -
Species: Rat
Sample Types: Cell Lysates
Applications: Western BlotTA Shah et al. (2021-02-12), Therapeutic Hypothermia Inhibits the Classical Complement Pathway in a Rat Model of Neonatal Hypoxic-Ischemic Encephalopathy Frontiers in Neuroscience, 2021-015(0):616734.
PMID: 33642979 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotT Guerenne-D et al. (2020-10-07), Multiplex coherent anti-Stokes Raman scattering microspectroscopy detection of lipid droplets in cancer cells expressing TrkB Sci Rep, 2020-110(1):16749.
PMID: 33028922 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotQ Zheng et al. (2020-10-02), Overexpression of microRNA-367 inhibits angiogenesis in ovarian cancer by downregulating the expression of LPA1 Cancer Cell Int, 2020-120(0):476.
PMID: 33024414 -
Species: Hamster
Sample Types: Cell Culture Supernates
Applications: Western BlotF Pennarubia et al. (2020-08-28), Mouse WIF1 Is Only Modified with O-Fucose in Its EGF-like Domain III Despite Two Evolutionarily Conserved Consensus Sites Biomolecules, 2020-010(9).
PMID: 32872229 -
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western BlotJ Tan et al. (2020-04-25), scAAV2-Mediated C3 Transferase Gene Therapy in a Rat Model with Retinal Ischemia/Reperfusion Injuries Mol Ther Methods Clin Dev, 2020-017(0):894-903.
PMID: 32382585 -
Species: Human
Sample Types: Cell
Applications: Western BlotJ Tan et al. (2019-11-29), C3 Transferase-Expressing scAAV2 Transduces Ocular Anterior Segment Tissues and Lowers Intraocular Pressure in Mouse and Monkey Mol Ther Methods Clin Dev, 2019-117(0):143-155.
PMID: 31909087 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotL Liu et al. (2019-11-18), PDHA1 Gene Knockout In Human Esophageal Squamous Cancer Cells Resulted In Greater Warburg Effect And Aggressive Features In Vitro And In Vivo Onco Targets Ther, 2019-112(0):9899-9913.
PMID: 31819487 -
Species: Mouse
Sample Types: Cell Lysates
Applications: Western BlotA Der Vartan et al. (2019-09-06), Downregulation of POFUT1 Impairs Secondary Myogenic Fusion Through a Reduced NFATc2/IL-4 Signaling Pathway Int J Mol Sci, 2019-020(18).
PMID: 31500188 -
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western BlotMS Abdelfatta et al. (2019-06-13), Prodigiosins from a marine sponge-associated actinomycete attenuate HCl/ethanol-induced gastric lesion via antioxidant and anti-inflammatory mechanisms PLoS ONE, 2019-014(6):e0216737.
PMID: 31194753 -
Species: Mouse
Sample Types: Tissue Lysates
Applications: Western BlotT Liu et al. (2019-01-21), Cysteinyl leukotriene receptor 2 drives lung immunopathology through a platelet and high mobility box 1-dependent mechanism Mucosal Immunol, 2019-00(0).
PMID: 30664709 -
Species: Mouse
Sample Types: Cell Lysates
Applications: Western BlotB Ilyas et al. (2018-10-23), Regulatory Evolution Drives Evasion of Host Inflammasomes by Salmonella Typhimurium Cell Rep, 2018-125(4):825-832.e5.
PMID: 30355489 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western BlotL Périè et al. (2017-12-08), Alterations in Adiposity and Glucose Homeostasis in Adult Gasp-1 Overexpressing Mice Cell. Physiol. Biochem., 2017-144(5):1896-1911.
PMID: 29224025 -
Species: Human
Sample Types: Whole Cells
Applications: Western BlotMK Shim et al. (2017-11-30), Caspase-3/-7-Specific Metabolic Precursor for Bioorthogonal Tracking of Tumor Apoptosis Sci Rep, 2017-17(1):16635.
PMID: 29192289 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotX Li et al. (2017-07-11), Metabolic reprogramming is associated with flavopiridol resistance in prostate cancer DU145 cells Sci Rep, 2017-07(1):5081.
PMID: 28698547 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotR Ganesan et al. (2017-02-13), Salmonella Typhimurium disrupts Sirt1/AMPK checkpoint control of mTOR to impair autophagy PLoS Pathog, 2017-013(2):e1006227.
PMID: 28192515 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotCT Yang et al. (2017-01-19), Activation of KLF1 Enhances the Differentiation and Maturation of Red Blood Cells from Human Pluripotent Stem Cells Stem Cells, 2017-035(4):886-897.
PMID: 28026072 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western BlotWang L et al. (2014-05-27), Up-regulation of VEGF by retinoic acid during hyperoxia prevents retinal neovascularization and retinopathy. Invest Ophthalmol Vis Sci, 2014-055(7):4276-87.
PMID: 24867581 -
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Western BlotChen et al. (2014-01-09), Prenatal retinoid deficiency leads to airway hyperresponsiveness in adult mice. J Clin Invest, 2014-0124(2):801-11.
PMID: 24401276 -
Species: Rat
Sample Types: Cell Lysates
Applications: Western BlotZhi et al. (2013-12-11), Effects of direct Renin inhibition on myocardial fibrosis and cardiac fibroblast function. PLoS ONE, 2013-18(12):e81612.
PMID: 24349097 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotLiang D et al. (2012-05-29), The hypoxic microenvironment upgrades stem-like properties of ovarian cancer cells. BMC Cancer, 2012-012(0):201.
PMID: 22642602 -
Species: Human
Sample Types: Tissue Homogenates
Applications: Western BlotDungwa JV et al. (2011-09-12), Overexpression of carbonic anhydrase and HIF-1alpha in Wilms tumours. BMC Cancer, 2011-011(0):390.
PMID: 21910893
There are no citations that match your criteria.
Customer Reviews for Human/Mouse/Rat GAPDH Antibody (9)
4.9 out of 5
9 customer
ratings
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Application: Western BlotSample Tested: Colon tissueSpecies: MouseVerified Customer | Posted 02/23/2022
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Application: Simple WesternSample Tested: HT-29 human colon adenocarcinoma cell lineSpecies: HumanVerified Customer | Posted 09/28/2020
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Application: Simple WesternSample Tested: human liver cell, Liver tissue, Skin tissue and Muscle tissueSpecies: Human and PigVerified Customer | Posted 08/05/2019
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Application: Western BlotSample Tested: 4T1 mouse breast cancer cell line and EMT6 cellsSpecies: MouseVerified Customer | Posted 10/17/2018
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Application: Western BlotSample Tested: HEK293 human embryonic kidney cell lineSpecies: HumanVerified Customer | Posted 07/21/2018
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Application: Western BlotSample Tested: IPS2 induced pluripotent stem cellsSpecies: MouseVerified Customer | Posted 01/25/2018
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Application: Western BlotSample Tested: Lysate and purified proteinSpecies: HumanVerified Customer | Posted 05/16/2017The blot contains HeLa cell lysate (1, 2, 3 and 5 µg) spiked with 200 ng of human transferrin per lane. Transferrin is detected in the red channel, tubulin is detected in the green channel and GAPDH is detected in the blue channel.
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Application: Western BlotSample Tested: See PMID 22868230Species: MouseVerified Customer | Posted 01/08/2015
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Application: Western BlotSample Tested: See PMID 23841780Species: MouseVerified Customer | Posted 01/08/2015
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