Species Reactivity:

Human, Mouse, Rat

Immunogen:

E. coli-derived recombinant human ERK2
Met1-Ser360
Accession # P28482

Applications:

Immunohistochemistry, Knockout Validated, Western Blot

Label:

Alexa Fluor 488 (Excitation=488 nm, Emission=515-545 nm)

Specificity:

Detects human, mouse, and rat ERK2. Also recognizes ERK1 in some cell types at much lower affinity.

View Full Technical Details for Human/Mouse/Rat ERK2 Alexa Fluor® 488-conjugated Antibody

Technical Data
Citations / Reviews

Applications

	Recommended Concentration	Sample
Western Blot	Optimal dilution of this antibody should be experimentally determined.
Immunohistochemistry	Optimal dilution of this antibody should be experimentally determined.

Item	Value
Knockout Validated	Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Antigen and protein A Affinity-purified
Formulation	Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

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Background: ERK2

ERK1 and ERK2 (also known as MAPK3 and MAPK1) are 44- and 42-kDa Ser/Thr kinases, respectively. They are part of the Ras-Raf-ERK signal transduction cascade often found downstream of growth factor receptor activation. ERK1 and ERK2 were initially isolated and cloned as kinases activated in response to insulin and NGF. They are expressed in most, if not all, mammalian tissues. Dual threonine and tyrosine phosphorylation activate both ERKs, at Thr202/Tyr204 for human ERK1 and Thr185/Tyr187 for human ERK2. Within the range used as an immunogen, human, mouse, and rat ERK1 share 100% amino acid sequence identity.

Item	Value
Long Name	Extracellular Signal-regulated Kinase 2
Entrez Gene IDs	5594 (Human); 26413 (Mouse); 116590 (Rat)
Alternate Names	EC 2.7.11, EC 2.7.11.24, ERK, ERK-2, ERK2, ERK2MAP kinase isoform p42, ERT1, Extracellular signal-regulated kinase 2, MAP kinase 1, MAP kinase 2, MAPK 1, MAPK1, MAPK2, Mitogen-activated protein kinase 2, PRKM1, PRKM1MAPK 2, PRKM2, mitogen-activated protein kinase 1, p38, p40, p41, p41mapk, p42-MAPK, p42mapk, protein tyrosine kinase ERK2

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Human/Mouse/Rat ERK2 Alexa Fluor® 488-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # AF1230G

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