Human/Mouse PDX-1/IPF1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2419
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Western Blot, Intracellular Staining by Flow Cytometry, Immunocytochemistry
Cited:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 267712
Product Specifications
Immunogen
E. coli-derived recombinant human PDX-1
Ala91-Arg283
Accession # P52945
Ala91-Arg283
Accession # P52945
Specificity
Detects human and mouse PDX-1 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse PDX-1/IPF1 Antibody
Detection of Mouse PDX‑1/IPF1 by Western Blot.
Western blot shows lysates of betaTC-6 mouse beta cell insulinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse PDX-1/IPF1 Monoclonal Antibody (Catalog # MAB2419) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PDX-1/IPF1 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
PDX‑1/IPF1 in betaTC‑6 Mouse Cell Line.
PDX-1/IPF1 was detected in immersion fixed betaTC-6 mouse beta cell insulinoma cell line using Human/Mouse PDX-1/IPF1 Monoclonal Antibody (Catalog # MAB2419) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
PDX‑1/IPF1 in BG01V Human Embryonic Stem Cells.
PDX-1/IPF1 was detected in immersion fixed BG01V human embryonic stem cells differentiated into pancreatic progenitor cells using Mouse Anti-Human/Mouse PDX-1/IPF1 Monoclonal Antibody (Catalog # MAB2419) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.Applications for Human/Mouse PDX-1/IPF1 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed betaTC-6 mouse beta cell insulinoma cell line and BG01V human embryonic stem cells differentiated into pancreatic progenitor cells
Sample: Immersion fixed betaTC-6 mouse beta cell insulinoma cell line and BG01V human embryonic stem cells differentiated into pancreatic progenitor cells
Immunohistochemistry
3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreas
Sample: Immersion fixed paraffin-embedded sections of human pancreas
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Sample: betaTC-6 mouse beta cell insulinoma cell line fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).
Sample: betaTC-6 mouse beta cell insulinoma cell line fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).
Western Blot
1 µg/mL
Sample: betaTC-6 mouse beta cell insulinoma cell line
Sample: betaTC-6 mouse beta cell insulinoma cell line
Reviewed Applications
Read 3 reviews rated 4.3 using MAB2419 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PDX-1/IPF1
Long Name
Pancreas/Duodenum Homeobox-1
Alternate Names
IDX1, IPF1
Gene Symbol
PDX1
UniProt
Additional PDX-1/IPF1 Products
Product Documents for Human/Mouse PDX-1/IPF1 Antibody
Product Specific Notices for Human/Mouse PDX-1/IPF1 Antibody
For research use only
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