Western blot shows lysates of human brain tissue, Jurkat human acute T cell leukemia cell line, HepG2 human hepatocellular carcinoma cell line, and BaF3 mouse pro-B cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse COX4-I1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5814) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for COX4-I1 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

COX4-I1 in Human Kidney.

COX4-I1 was detected in immersion fixed paraffin-embedded sections of normal human kidney using Goat Anti-Human/Mouse COX4-I1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5814) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

COX4‑I1 in HeLa Human Cell Line.

COX4-I1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse COX4-I1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5814) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to mitochondria. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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Applications for Human/Mouse COX4-I1 Antibody

Application

Recommended Usage

Immunocytochemistry

This antibody has been used at a concentration of 5-15 μg/mL to detect COX4-I1 in immersion fixed HeLa human cervical epithelial carcinoma cell line. Internal testing was not able to validate staining in a mouse cell line.

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of normal human kidney

Simple Western

10 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and HepG2 human hepatocellular carcinoma cell line

Western Blot

1 µg/mL
Sample: Human brain tissue, Jurkat human acute T cell leukemia cell line, HepG2 human hepatocellular carcinoma cell line, and BaF3 mouse pro-B cell line

Reviewed Applications

Read 2 reviews rated 4 using AF5814 in the following applications:

Immunofluorescence (2 Reviews)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: COX4-I1

Cytochrome c oxidase subunit 4 isoform 1 (COX-4I1) is a 21‑22 kDa member of the cytochrome c oxidase IV family of proteins. It is a component of COX, an inner mitochondrial membrane multimeric dimer that catalyzes the transfer of electrons from cytochrome c to dioxygen. COX-4I1 is the largest of 10 distinct nuclear DNA‑encoded subunits that form each COX monomer. Human COX4-I1 is 169 amino acids (aa) in length and possesses a mitochondrial transit peptide between aa 1‑22, an ATP binding site (aa 42; 95‑100), and multiple subunit interface sequences. The ATP binding site is suggested to make COX-4I1 a regulatory subunit within the COX complex. There are two potential splice variants for COX-4I1 that show a three aa substitution for aa 81‑169, and a single Gly substitution for aa 125‑169. COX‑4I2, the product of a different gene, shares only 50% aa identity with COX-4I1. Over amino acid 23‑169, human COX4‑1 shares 78% aa identity with mouse COX‑4I1.

Long Name

Cytochrome c Oxidase Subunit IV Isoform 1

Alternate Names

COX IV-1, COX4I1

Entrez Gene IDs

1327 (Human); 12857 (Mouse); 29445 (Rat)

Gene Symbol

COX4I1

UniProt

P13073 (Human)

Additional COX4-I1 Products

Product Documents for Human/Mouse COX4-I1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse COX4-I1 Antibody

For research use only

Citations
Reviews

Human/Mouse COX4-I1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5814

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all COX4-I1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse COX4-I1 Antibody

Detection of Human/Mouse COX4-I1 by Western Blot.