Detects human MMP-1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross-reactivity with recombinant human (rh) MMP‑2, rhMMP-3, rhMMP-7, rhMMP-8, rhMMP-9, rhMMP-10, rhMMP-12, rhMMP-13, and rhMMP-16 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human MMP-1 Antibody

Detection of Human MMP‑1 by Western Blot.

Western blot shows lysates of PC-3 human prostate cancer cell line and HEK001 human epidermal keratinocyte cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MMP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF901) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MMP-1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human MMP-1 by Simple Western^TM.

Left: Simple Western lane view shows lysates of MDA-MB-231 human breast cancer cell line, loaded at 0.1 mg/ml. A specific band was detected for MMP-1 at approximately 55 kDa (as indicated) using both 10 µg/ml and 50 µg/ml of Goat Anti-Human MMP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF901) followed by HRP-conjugated Donkey Anti-Goat Secondary Antibody (Catalog # 043-522-2). This experiment was conducted under reducing conditions and using the 12-230kDa separation system. Right: Simple Western electropherogram showing the same Goat Anti-Human MMP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF901) tested at 10 µg/ml (blue line) and 50 µg/ml (green line) in the MDA-MB-231 human breast cancer cell line.

MMP‑1 in Human Ovarian Cancer Tissue.

MMP-1 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 15 µg/mL Goat Anti-Human MMP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF901) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

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Applications for Human MMP-1 Antibody

Application

Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human ovarian cancer tissue and immersion fixed paraffin-embedded sections of human prostate

Knockout Validated

MMP-1 is specifically detected in PC‑3 human prostate cancer parental cell line but is not detectable in MMP-1 knockout PC‑3 cell line.

Simple Western

10-50 µg/mL
Sample: MDA-MB-231 human breast cancer cell line

Western Blot

1 µg/mL
Sample: PC‑3 human prostate cancer cell line and HEK001 human epidermal keratinocyte cell line

Human MMP-1 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)

Recommended Concentration: 0.2-0.8 µg/mL
Use in combination with these reagents:

Detection Reagent: Human MMP-1 Biotinylated Antibody (Catalog # BAF901)
Standard: Recombinant Human MMP-1 Protein, CF (Catalog # 901-MP)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4 using AF901 in the following applications:

ELISA (1 Review)
Immunohistochemistry (1 Review)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-1

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-1 (interstitial collagenase), can degrade a broad range of substrates including types I, II, III, VII, VIII, and X collagens as well as casein, gelatin,
alpha-1 antitrypsin, myelin basic protein, L-Selectin, pro-TNF, IL-1 beta, IGFBP-3, IGFBP-5, pro-MMP-2, and pro-MMP-9. A significant role of MMP-1 is the degradation of fibrillar collagens in extracellular matrix remodeling, characterized by the cleavage of the interstitial collagen triple helix into ¾, ¼ fragments. However, as the list of substrates above illustrates, the role of MMP-1 is more diverse than originally envisaged, and may involve enzyme cascades, cytokine regulation, and cell surface molecule modulation. MMP-1 is expressed by fibroblasts, keratinocytes, endothelial cells, monocytes, and macrophages. Structurally, MMP-1 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain.

References

Cawston, T.E. (2004) in Interstitial Collagenase. Barrett, A.J. et al. (eds): Handbook of Proteolytic Enzymes, San Diego: Academic Press, p. 472.

Long Name

Matrix Metalloproteinase 1

Alternate Names

MMP1

Entrez Gene IDs

4312 (Human); 83995 (Mouse)

Gene Symbol

MMP1

UniProt

P03956

Additional MMP-1 Products

Product Documents for Human MMP-1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human MMP-1 Antibody

For research use only

Citations
Reviews

Human MMP-1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF901

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications